{"id":"hum0304","version":1,"url":"https://humandbs.dbcls.jp/research/hum0304/v1","datePublished":"2022-04-08","versions":[{"version":1,"datePublished":"2022-04-08"}],"title":{"ja":"乳がんにおけるタキサン作用機序の解明に関する研究","en":"Exome sequencing of human breast cancer tissues resistant to taxanes."},"summary":{"aims":{"ja":"タキサン系抗がん剤は乳がん治療におけるキードラッグの一つであり、乳がん治療における初期および再発治療を含むすべての段階で重要な役割を担う薬剤である。しかし、タキサン系抗がん剤の投与により腫瘍が増大傾向を示す乳がんがまれながら経験され、ひとつの臨床的課題となっている。本研究は、タキサン系抗がん剤投与により腫瘍が増大傾向を示した症例を選別し、網羅的にwhole exome sequencing解析を実施することで共通する体細胞変異を見出し、その機能解析を通してタキサン作用メカニズムの解明と薬剤不応の克服を目指す。","en":"Taxanes are key drugs in the treatment of breast cancer, but some breast cancers are resistant to taxanes. We used whole exome-sequencing (WES) technology to investigate mechanisms of taxane resistance in human breast cancer."},"methods":{"ja":"タキサン系抗がん剤投与により腫瘍が増大傾向を示した症例を選別し、乳がん組織ならびに血液組織から抽出したDNAを対象に網羅的にwhole exome sequencing解析を行い、腫瘍組織に特異的な変異を検出する。","en":"DNAs extracted from tumor tissues and peripheral blood cells of patients with breast cancer whose tumors responded well to anthracycline treatment but grew rapidly during neoadjuvant taxane-based chemotherapy were used for WES analysis and somatic mutations in the candidate genes were identified."},"targets":{"ja":"タキサン系抗がん剤投与により腫瘍が増大傾向を示した乳がん症例","en":"Six patients with breast cancer whose tumors grew rapidly during neoadjuvant taxane-based chemotherapy"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"乳癌：6症例\n（日本人）","en":"6 breast cancers\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome）","en":"NGS\n(Exome)"}},"releaseNote":{"ja":"タキサン系抗がん剤投与により腫瘍が増大傾向を示した乳がん症例の腫瘍組織および末梢血から抽出したDNAを用いたwhole exome sequencing解析データの結果をfastqファイルにて提供する。SureSelect Human ALL Exon V5 KitによりDNAライブラリを作成し、Illumina HiSeq 2000/2500にて平均長200塩基を解読した（paired-end）。","en":"DNAs extracted from tumor tissue and peripheral blood cells of 6 patients with breast cancer whose tumors grew rapidly during neoadjuvant taxane-based chemotherapy were used for the whole exome sequencing analysis. DNA libraries were established with SureSelect Human ALL Exon V5 Kit and sequenced by Illumina HiSeq 2000/2500 (paired-end; 200 bp). Fastq files are provided."},"dataProviders":[{"name":{"ja":"鰐渕 友美","en":"Yumi Wanifuchi-Endo"},"organization":{"name":{"ja":"名古屋市立大学大学院 医学研究科 乳腺外科学分野","en":"Department of Breast Surgery, Graduate School of Medical Sciences, Nagoya City University"}}}],"researchProjects":[],"grants":[{"title":{"ja":"乳癌におけるタキサン耐性機序の解明と新規分子標的治療薬の開発","en":"Exome sequencing analysis in human breast cancer tissues showing resistance for taxanes."},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["16K10469"]}],"relatedPublications":[{"title":"Discovering novel mechanisms of taxane resistance in human breast cancer by whole-exome sequencing","doi":"https://doi.org/10.3892/ol.2021.13178","datasets":["JGAD000484"]}],"datasets":["JGAD000484"],"controlledAccessUsers":[]}