{"id":"hum0302","version":2,"url":"https://humandbs.dbcls.jp/research/hum0302/v2","datePublished":"2024-03-18","versions":[{"version":1,"datePublished":"2022-09-05"},{"version":2,"datePublished":"2024-03-18"}],"title":{"ja":"iPS細胞の高次特性解析と加工iPS細胞の作製","en":"iPS Cell Advanced Characterization and Development of modified iPS cells"},"summary":{"aims":{"ja":"iPS細胞株（健常人及び疾患特異的iPS細胞株）を使用し、分化能解析（疾患原因細胞や関与細胞への分化能の評価）、疾患原因遺伝子の解析、全ゲノム解析等の高次特性解析を実施する。また、ゲノム編集技術等を利用して（1）疾患原因遺伝子を正常遺伝子に置換した疾患特異的iPS細胞株（isogenic control cell）、疾患特異的iPS細胞における疾患原因遺伝子を正常遺伝子に置換した細胞（isogenic control cell）、（2）正常遺伝子を疾患原因遺伝子に置換した細胞株（人工作製疾患特異的iPS細胞株）、（3）組織特異的及び/又は分化段階特異的にマーカー（蛍光マーカー等）を発現する加工iPS細胞株を作製する。","en":"To characterize iPS cell lines (healthy-donor iPS cell lines and disease-specific iPS cell lines), differentiation potential analysis (evaluation of differentiation potential into disease-causing cells and involved cells), disease-causing gene analysis, and whole genome analysis were performed."},"methods":{"ja":"【JGAS000382】ヒトiPS細胞から肝細胞を分化誘導し、19日目に回収。RNAを抽出後PolyA選択法により作製したストランド特異的ライブラリーのシークエンシング解析をNovaseq6000にて行った（サンプルあたり約6Gb [約2000万ペアリード相当]のデータ出力）。\n【JGAS000683】ヒトiPS細胞から腎臓オルガノイドを分化誘導し、18日目に回収。RNAを抽出後PolyA選択法により作製したストランド特異的ライブラリーのシークエンシング解析をNovaseq6000にて行った（サンプルあたり約6Gb [約2000万ペアリード相当]のデータ出力）。","en":"[JGAS000382] Hepatocytes were induced to differentiate from human iPS cells and collected on day 19. RNA was collected and strand-specific library preparation was performed by a PolyA selection method. The prepared library was sequenced by Novaseq6000. Sequencing was performed in a 2x150 bp PE configuration with a data output of about 6 Gb per sample (equivalent to about 20 million paired reads).\n[JGAS000683] Kidney organoids were induced to differentiate from human iPS cells and collected on day 18. RNA was collected and strand-specific library preparation was performed by a PolyA selection method. The prepared library was sequenced by Novaseq6000. Sequencing was performed in a 2x150 bp PE configuration with a data output of about 6 Gb per sample (equivalent to about 20 million paired reads)."},"targets":{"ja":"ウイルソン病（指定難病171）患者由来iPS細胞株、若年性ネフロン癆（NPH1）患者由来iPS細胞株ならびに、健常人由来iPS細胞株","en":"iPS cells derived from patients with Wilson's disease, juvenile nephron tabes (NPH1) patients and iPS cells derived from healthy donors"},"url":{"ja":[{"url":"https://acd.brc.riken.jp/ja/","text":"https://acd.brc.riken.jp/ja/"}],"en":[{"url":"https://acd.brc.riken.jp/en/","text":"https://acd.brc.riken.jp/en/"}]}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"ウイルソン病患者由来iPS細胞株：6検体\n若年性ネフロン癆（NPH1）患者由来iPS細胞株：12検体\n健常人由来iPS細胞株：6＋6検体\n（細胞株）","en":"iPS cells derived from patients with Wilson's disease: 6 samples\niPS cells derived from patients with juvenile nephron tabes (NPH1): 12 samples\niPS cells derived from healthy donors: 6+6 samples\n(Cell-line)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"若年性ネフロン癆（NPH1）由来iPS細胞および健常人由来iPS細胞から分化誘導させた腎臓オルガノイドサンプルから抽出したRNAを用いたRNA-seq解析の結果をfastqファイルにて提供する。","en":"RNAs extracted from kidney organoids differentiated from iPS cells derived from patients with juvenile nephron tabes (NPH1) and iPS cells derived from healthy donors were used for RNA-sequencing analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"林 洋平","en":"Yohei Hayashi"},"organization":{"name":{"ja":"理化学研究所 バイオリソース研究センター","en":"RIKEN BioResource Research Center"}}}],"researchProjects":[{"name":{"ja":"iPS細胞高次特性解析開発チーム","en":"iPS Cell Advanced Characterization and Development Team"},"url":{"ja":[{"url":"https://acd.brc.riken.jp/ja/","text":"https://acd.brc.riken.jp/ja/"}],"en":[{"url":"https://acd.brc.riken.jp/en/","text":"https://acd.brc.riken.jp/en/"}]}}],"grants":[],"relatedPublications":[{"title":"Retinoids rescue ceruloplasmin secretion and alleviate oxidative stress in Wilson's disease-specific hepatocytes.","doi":"https://doi.org/10.1093/hmg/ddac080","datasets":["JGAD000497"]}],"datasets":["JGAD000497","JGAD000816"],"controlledAccessUsers":[]}