{"id":"hum0298","version":1,"url":"https://humandbs.dbcls.jp/research/hum0298/v1","datePublished":"2021-10-18","versions":[{"version":1,"datePublished":"2021-10-18"}],"title":{"ja":"慢性胃炎から胃の発がんや進展に深く関与しうる遺伝子並びに非コードRNAの量的・質的異常の探索","en":"Screening for quantitative and qualitative abnormalities of long non-coding RNA associated with gastritis and gastric carcinogenesis"},"summary":{"aims":{"ja":"胃がんの発生・発育進展過程における腫瘍細胞の分子異常を網羅的に探索することで発がん・がんの進展に深く関与しうるlong non-coding RNA（lncRNA）の分子異常の同定と機能解明を目的とする。さらにlncRNAと臨床病理学的因子との関連を明らかにすることで、がん予防・早期発見を目指した発がんリスク予測マーカーの開発や、治療方法の選択マーカー開発などの臨床応用を目指す。","en":"This study aimed to identify dysregulations of long non-coding RNA (lncRNA) in gastric cancer, and to reveal molecular functions of lncRNAs associated with gastritis and gastric carcinogenesis. We also aimed to reveal the correlation between clinicopathological characteristics and lncRNA alterations. To this end, we will try to develop predictive markers of gastric cancer risk, which enables prevention and early detection of gastric cancer. We will also try to develop molecular markers which enable us to select treatment strategies for gastric cancer."},"methods":{"ja":"胃がん患者から摘出した正常胃粘膜とがん部の17個の凍結組織からDNA抽出した。胃がんで発現異常を示した17個のlncRNAと11個の胃がんがん遺伝子を対象にしたカスタムパネルを作成した。抽出したDNAからアンプリコンシークエンス用のライブラリーを作成し、カスタムパネルを用いてIon Proton sequencerによるシークエンス解析を行った。","en":"Genomic DNA was extracted from 17 fresh frozen tissues, containing pairs of non-cancerous gastric mucosa and tumor tissues obtained from 8 patients. We designed a custom AmpliSeq panel that targeted 28 genes. The genes were selected 17 lncRNAs which were aberrantly expressed in gastric cancer and 11 oncogenes. Libraries for amplicon sequencing were prepared using the extracted DNAs according to the manufacturer's instructions. Amplicon sequencing was performed using an Ion Proton sequencer (Thermo Fisher Scientific)."},"targets":{"ja":"内視鏡検査を行い、胃がん患者から非がん部背景粘膜と腫瘍組織の検体を収集した。生検検体は8名の胃がん患者から採取した胃がん組織9個と正常胃粘膜8個であった。","en":"Non-cancerous gastric mucosa and tumor tissues specimens were obtained from Japanese patients by performing an endoscopic biopsy. Biopsy specimens were nine tumor tissues and eight gastric mucosae obtained from eight patients with gastric cancer."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"胃がん患者：8名 （日本人）","en":"8 patients with gastric cancer (Japanese)"},"typeOfData":{"ja":"NGS\n（Amplicon-seq）","en":"NGS\n(Amplicon-seq)"}},"releaseNote":{"ja":"胃がん8症例の腫瘍組織および非腫瘍組織から抽出したDNAを用いた、17個のlncRNAと11個の胃がん遺伝子を対象にしたカスタムパネルシークエンス解析の結果をfastqファイルにて提供する。","en":"DNAs extracted from tumor and non-tumor tissues collected from 8 patiets with gastric cancer were used for Amplicon sequencing with a custom panel for 17 lncRNAs and 11 oncogenes. Fastq files are provided."},"dataProviders":[{"name":{"ja":"北嶋 洋志","en":"Hiroshi Kitajima"},"organization":{"name":{"ja":"札幌医科大学医学部 分子生物学講座","en":"Department of Molecular Biology, Sapporo Medical University School of Medicine"}}}],"researchProjects":[],"grants":[{"title":{"ja":"先進ゲノム解析研究推進プラットフォーム","en":"Platform for Advanced Genome Science"},"agency":{"ja":"科学研究費助成事業 新学術領域研究","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas"},"grantIds":["16H06279"]}],"relatedPublications":[],"datasets":["JGAD000467"],"controlledAccessUsers":[]}