{"id":"hum0289","version":1,"url":"https://humandbs.dbcls.jp/research/hum0289/v1","datePublished":"2022-09-09","versions":[{"version":1,"datePublished":"2022-09-09"}],"title":{"ja":"慢性骨髄単球性白血病（CMML）における同種移植後再発メカニズムの探索","en":"Mechanisms on relapse after allogeneic hematopoietic cell transplantation in CMML"},"summary":{"aims":{"ja":"次世代シーケンサーなどを使用した網羅的な解析を行うことで、慢性骨髄単球性白血病（CMML）症例における同種造血幹細胞移植後再発メカニズムの同定を行う。","en":"Disease relapse is a major cause of treatment failure after allogeneic hematopoietic cell transplantation (HCT), and mechanisms on relapse still remain to be elucidated. We experienced a patient with chronic myelomonocytic leukemia (CMML) who underwent haploidentical HCT from his daughter and developed relapse. To understand the mechanisms on relapse after HCT, we analyze blood samples at HCT and at relapse."},"methods":{"ja":"移植時および再発時の末梢血サンプルより分離した腫瘍由来のCD14+/CD16-単球から抽出したDNA/RNAを用いたRNAシークエンス、エクソンシークエンス、メチル化アレイ解析","en":"We analyzed peripheral blood samples that had been collected at HCT and relapse. CD14+/CD16- monocytes that accumulate typically in CMML were isolated by flow cytometry. Whole-exome sequencing, RNA sequencing, and methylation array were performed to determine the mechanism of relapse."},"targets":{"ja":"慢性骨髄単球性白血病（CMML）1症例、同種造血幹細胞移植前と移植後再発時の末梢血サンプル（1症例2サンプル）","en":"chronic myelomonocytic leukemia (CMML): 1 case; peripheral blood samples that had been collected at allogeneic hematopoietic cell transplantation and relapse (2 samples)"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定\n発現\nメチル化","en":"Sequencing, Expression profiling, Methylation profiling"},"targets":{"ja":"慢性骨髄単球性白血病（CMML）：1症例\n（日本人）","en":"chronic myelomonocytic leukemia (CMML): 1 case\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq、Exome）\nメチル化アレイ","en":"NGS\n(RNA-seq, Exome)\nMethylation array"}},"releaseNote":{"ja":"慢性骨髄単球性白血病（CMML）1症例の 同種造血幹細胞移植前と移植後再発時の末梢血より単離したCD14+･CD16-単球から抽出したDNA/RNAを用いたRNA-seq、Exome、メチル化アレイ解析の結果をbam、bpmファイル形式で提供する。","en":"RNA-seq, Exome and DNA methylation array in a patient with chronic myelomonocytic leukemia (CMML) were performed using DNA/RNAs extracted from CD14+/CD16- monocytes isolated from peripheral blood samples collected at HCT and relapse. The data are provided as bam and bpm format files."},"dataProviders":[{"name":{"ja":"赤星 佑","en":"Yu Akahoshi"},"organization":{"name":{"ja":"自治医科大学附属さいたま医療センター 血液科","en":"Saitama Medical Center, Jichi Medical University"}}}],"researchProjects":[],"grants":[],"relatedPublications":[{"title":"Emergence of clone with PHF6 nonsense mutation in chronic myelomonocytic leukemia at relapse after allogeneic HCT","doi":"https://doi.org/10.1007/s12185-021-03284-7","datasets":["JGAD000428"]}],"datasets":["JGAD000428"],"controlledAccessUsers":[]}