{"id":"hum0284","version":1,"url":"https://humandbs.dbcls.jp/research/hum0284/v1","datePublished":"2023-01-19","versions":[{"version":1,"datePublished":"2023-01-19"}],"title":{"ja":"生体維持機能の破綻による前がん病変から浸潤がんへの移行を制御する分子機序の解明","en":"Elucidation of the molecular mechanism that controls the transition from precancerous lesions to invasive cancer due to disruption of biological maintenance function"},"summary":{"aims":{"ja":"乳がん臨床検体の1細胞解析を通して、前がん病変から発がん悪性化に至る遺伝子発現領域の変化を明らかにする","en":"To clarify expressional changes from precancerous to malignancy by single-cell RNA-seq analysis of breast cancer clinical specimens"},"methods":{"ja":"外科的切除された腫瘍組織から細胞を単離し、10xChromiumを用いてシングルセルライブラリを構築する。作製したライブラリに対して次世代シーケンサーを用いた1細胞遺伝子発現解析を行う。","en":"Cells were isolated from the surgically resected tumor tissue. Single-cell sequence library was constructed using 10xChromium kit. Single-cell gene expression analysis was performed by a next-generation sequencer."},"targets":{"ja":"乳がん症例より採取した原発腫瘍組織","en":"Primary tumor samples collected from breast cancer patients"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"乳癌：4症例","en":"Breast cancer: 4 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq）","en":"NGS\n(scRNA-seq)"}},"releaseNote":{"ja":"乳がん症例より採取した原発腫瘍組織から単離した細胞より抽出したRNAを用いてsingle cell RNA-seq解析したデータをfastqファイルにて提供する。","en":"RNAs extracted from surgically resected tumor tissues of breast invasive carcinoma patients were used for single-cell RNA-sequencing analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"中山 淳","en":"Jun Nakayama"},"organization":{"name":{"ja":"国立がん研究センター 研究所細胞情報学分野","en":"Division of Cellular Signaling, National Cancer Center Research Institute"}}}],"researchProjects":[{"name":{"ja":"病態情報学ユニット","en":"Laboratory of Integrative Oncology"},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"シングルセルアンプリコミクスによる癌進化系譜の解明","en":"Analysis of Cancer Evolution by Single Cell Amplicomics"},"agency":{"ja":"科学研究費助成事業 特別研究員奨励費","en":"KAKENHI Grant-in-Aid for JSPS Research Fellows"},"grantIds":["20J01794"]}],"relatedPublications":[{"title":"Single-Cell Transcriptome Profiling Reveals Intratumoral Heterogeneity and Molecular Features of Ductal Carcinoma In Situ","doi":"https://doi.org/10.1158/0008-5472.can-22-0090","datasets":["JGAD000420"]}],"datasets":["JGAD000420"],"controlledAccessUsers":[]}