{"id":"hum0278","version":1,"url":"https://humandbs.dbcls.jp/research/hum0278/v1","datePublished":"2022-07-07","versions":[{"version":1,"datePublished":"2022-07-07"}],"title":{"ja":"乳癌幹細胞のゲノム解析","en":"Analysis of genome of breast cancer stem cells"},"summary":{"aims":{"ja":"乳がん幹細胞のゲノム解析およびシングルセル解析を用いた乳がんにおける不均一変異の解明","en":"Identification of heterogeneity of breast cancer stem cells using genomic and single cell analyses."},"methods":{"ja":"乳がん症例の腫瘍組織や、当該腫瘍組織を免疫不全マウスに移植したPatient-derived xenograft（PDX）モデルにおいて増殖した腫瘍組織からDNA/RNAを抽出し、whole exome sequencing（Exome）/ RNA-seq解析を実施","en":"Whole exome sequence analysis by using breast cancer cells sorted into cancer stem cells and non-cancer stem cells. Single cell RNA sequence of sorted breast cancer stem cells exploiting C1 system."},"targets":{"ja":"乳がん 5症例（scRNA-seq：4症例11検体、Exome：5症例12検体）","en":"Breast cancer: 5 cases (scRNA: 4 cases, 11 samples; exome: 5 cases, 12 samples)"},"url":{"ja":[{"url":"https://bunshibyotai.w3.kanazawa-u.ac.jp","text":"https://bunshibyotai.w3.kanazawa-u.ac.jp"}],"en":null}},"listingSummary":{"methods":{"ja":"配列決定\n発現","en":"Sequencing, Expression profiling"},"targets":{"ja":"乳癌：5症例\n（日本人）","en":"Breast cancer: 5 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome、scRNA-seq）","en":"NGS\n(Exome, scRNA-seq)"}},"releaseNote":{"ja":"乳がん臨床検体から得られたがん細胞をがん幹細胞と非がん幹細胞に分画し、各分画細胞から抽出したDNAを用いたWES解析データ、および、単一がん幹細胞から抽出したRNAを用いたsingle cell RNA-seq解析データをfastqファイル形式で提供する。","en":"- DNAs extracted from breast cancer stem cells and those extracted from breast non-cancer stem cells were used for whole exome sequencing analysis. Fastq files are provided.\n- RNAs extracted from each of breast cancer stem cells were used for single cell RNA sequencing analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"後藤 典子","en":"Noriko Gotoh"},"organization":{"name":{"ja":"金沢大学がん進展制御研究所 分子病態研究分野","en":"Division of Cancer Cell Biology, Cancer Research Institute, Kanazawa University"}}}],"researchProjects":[],"grants":[{"title":{"ja":"先進ゲノム解析研究推進プラットフォーム","en":"Platform for Advaced Genomic Science"},"agency":{"ja":"科学研究費助成事業 新学術領域研究","en":"Grant-in-Aid for Scientific Research on Innovative Areas - Platforms for Advanced Technologies and Research Resources"},"grantIds":["16H06279"]}],"relatedPublications":[{"title":"Addiction to the IGF2-ID1-IGF2 circuit for maintenance of the breast cancer stem-like cells.","doi":"https://doi.org/10.1038/onc.2016.293","datasets":null},{"title":"Semaphorin signaling via MICAL3 induces symmetric cell division to expand breast cancer stem-like cells.","doi":"https://doi.org/10.1073/pnas.1806851116","datasets":null}],"datasets":["JGAD000415","JGAD000416"],"controlledAccessUsers":[]}