{"id":"hum0274","version":1,"url":"https://humandbs.dbcls.jp/research/hum0274/v1","datePublished":"2022-04-22","versions":[{"version":1,"datePublished":"2022-04-22"}],"title":{"ja":"mRNA輸送因子UAP56とURH49による選択的mRNA輸送基盤の統合的解析","en":"Integrated analysis of selective mRNA export by mRNA export factors, UAP56 and URH49"},"summary":{"aims":{"ja":"mRNAの核外輸送はmRNA輸送体によって担われており、ヒトにおいてはNXF1とCRM1がmRNAの輸送に機能する。しかし両者が異なるmRNA群を輸送するか明らかではない。本研究は、NXF1とCRM1が輸送するmRNA群をゲノムワイドに同定し、NXF1とCRM1によるmRNA輸送の分子機構を明らかにする。","en":"mRNA exporters, NXF1 and CRM1, are known to function in mRNA export. However, since each target mRNA has not been identified genome-widely, it is not clear whether they export exclusive mRNA subsets. To reveal this point, we prepared the nuclear and the cytoplasmic RNAs under either exporter inhibited condition and carried out RNA-seq."},"methods":{"ja":"目的遺伝子をノックダウン（knockdown：KD）したHeLa細胞を核と細胞質に分画した後RNAを抽出し、RNA-seq解析を実施した。","en":"Knockdown of a target gene was performed in cultured human cells (HeLa), and RNA was extracted after fractionation into the nucleus and cytoplasm."},"targets":{"ja":"目的遺伝子KD後のHeLa細胞","en":"HeLa cells with knockdown of the target gene"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"目的遺伝子をノックダウンしたHeLa細胞：20検体\n（細胞株）","en":"HeLa cells with knockdown of the target genes: 20 samples\n(Cell-line)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"control KD、NXF1 KD、MTR4 KDそれぞれを実施したHeLa細胞に対し、LMB（CRM1阻害剤）処理の有無により培養し、核と細胞質へ分画後に抽出したRNAを用いたRNA-seq解析データ（fastqファイル形式）を提供する。","en":"RNAs extracted from nucleus or cytoplasm fractions obtained from HeLa cells which were knockdown of each target gene and cultured with/without LMB were used for RNA-sequencing analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"藤田 賢一","en":"Kenichi Fujita"},"organization":{"name":{"ja":"藤田医科大学 総合医科学研究所","en":"Division of Gene Expression Mechanism, Fujita Healthy University"}}}],"researchProjects":[],"grants":[{"title":{"ja":"mRNA輸送因子UAP56とURH49による選択的mRNA輸送基盤の統合的解析","en":"Integrated analysis of selective mRNA export by mRNA export factors, UAP56 and URH49"},"agency":{"ja":"科学研究費助成事業 若手研究","en":"KAKENHI Grant-in-Aid for Early-Career Scientists"},"grantIds":["19K15807"]}],"relatedPublications":[{"title":"MPP6 stimulates both RRP6 and DIS3 to degrade a specified subset of MTR4-sensitive substrates in the human nucleus","doi":"https://doi.org/10.1093/nar/gkac559","datasets":["JGAD000401"]}],"datasets":["JGAD000401"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000401"]}]}