{"id":"hum0273","version":1,"url":"https://humandbs.dbcls.jp/research/hum0273/v1","datePublished":"2021-08-27","versions":[{"version":1,"datePublished":"2021-08-27"}],"title":{"ja":"乳癌の発生、進展に関する分子病理学的研究","en":"A study of molecular pathology for carcinogenesis and progression of breast cancer"},"summary":{"aims":{"ja":"乳がんは多様な形態を示し、特に変異や遺伝子発現プロファイルが異なる様々な細胞型を持つことを特徴としている。管腔がんと小葉がんの両方の表現型からなる腫瘍におけるがん細胞のクローン関係を明らかにする。","en":"Breast cancer manifests in diverse forms, with particular reference to various cell types harboring different mutations and gene expression profiles. To elucidate the clonal relationship between cancer cells in tumors composed of both ductal and lobular phenotypes, we analyzed two combined lobular and ductal carcinoma cases by next generation sequencing."},"methods":{"ja":"サンプルDNAを製造業者のプロトコルに従って、Ion Torrentプラットフォーム（Thermo Fisher SCIENTIFIC）上のIon AmpliSeq Comprehensive Cancer Panelを用いて、アンプリコン配列決定に使用した。このパネルは、409個のがん関連遺伝子のすべてのエクソンをカバーする約16,000個のプライマー対から構成されている。DNA（120 ng）を使用して、IonXpressバーコードされたアダプターを用いてライブラリーを調製し、イオンチップを用いたシーケンシングには、イオンプロトンシーケンサー（Thermo Fisher SCIENTIFIC）を用いた。","en":"DNA from samples were used for amplicon sequencing by an Ion AmpliSeq Comprehensive Cancer Panel on an Ion Torrent platform (Thermo Fisher SCIENTIFIC). The panel consists of approximately 16,000 primer pairs covering all exons of 409 cancer-associated genes (1.6 megabases of target sequences). DNA (120 ng) was used to prepare the libraries with IonXpress barcoded adapters. An Ion Proton Sequencer (Thermo Fisher SCIENTIFIC) was used for sequencing with the ion chip."},"targets":{"ja":"小葉がんおよび乳管がん併存2症例の腫瘍組織ならびに周辺の非腫瘍組織からレーザーマイクロダイセクションにより採取した合計9検体","en":"A total of 9 DNAs collected by laser microdissection from 2 cases of lobular carcinoma in situ with ductal carcinoma in situ."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"小葉癌乳管癌併存症例：2症例\n（日本人）","en":"2 patients of lobular carcinoma in situ with ductal carcinoma in situ\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Amplicon-seq）","en":"NGS\n(Amplicon-seq)"}},"releaseNote":{"ja":"小葉がんおよび乳管がん併存2症例の腫瘍組織ならびに非腫瘍組織からDNAを抽出し、Ion AmpliSeq Comprehensive Cancer Panel（がん関連409遺伝子）を用いてAmplicon Sequencingを行った結果をbamファイルにて提供する。","en":"DNAs extracted from tumor tissues and and non-tumor tissues of 2 patients of lobular carcinoma in situ with ductal carcinoma in situ were used for an amplicon sequencing analysis of 409 cancer-related genes. Bam files are provided."},"dataProviders":[{"name":{"ja":"増田 しのぶ","en":"Shinobu Masuda"},"organization":{"name":{"ja":"日本大学医学部 病態病理学系 腫瘍病理学分野","en":"Division of Oncologic Pathology, Department of Pathology and Microbiology, Nihon University School of Medicine"}}}],"researchProjects":[],"grants":[{"title":{"ja":"組織型が混在する乳癌のミトコンドリアDNA変異解析による癌細胞系譜の解明","en":"A cell lineage of breast cancer consisting of different histologic types by mutation analysis of mtDNA D-loop region"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["15K08357"]}],"relatedPublications":[{"title":"Phylogenetic analysis of combined lobular and ductal carcinoma of the breast","doi":"https://doi.org/10.3892/mmr.2021.12357","datasets":["JGAD000411"]}],"datasets":["JGAD000411"],"controlledAccessUsers":[]}