{"id":"hum0257","secondaryIds":[],"version":3,"url":"https://humandbs.dbcls.jp/research/hum0257/v3","datePublished":"2026-11-04","versions":[{"version":1,"datePublished":"2021-12-24"},{"version":2,"datePublished":"2023-01-10"},{"version":3,"datePublished":"2026-11-04"}],"title":{"ja":"ヒト膵・胆道由来微量検体を用いた培養増幅法の研究開発","en":"Development of culturing methods amplifying micro-specimens derived from human pancreas and biliary tract tissues"},"summary":{"aims":{"ja":"癌の培養細胞株は、その再現性・簡便さから癌研究の中心を担ってきた。しかしながら、同じ癌種であっても腫瘍を構成する癌細胞は個人ごとに異なり、かつgeneticにもepigeneticにも不均一な細胞集団から構成されることが明らかとなってきた。培養細胞株は樹立の過程で培養条件に適した細胞が選択されるため、原発巣の腫瘍細胞の不均一性を正確に反映しない可能性が指摘されている。\n本研究では、生検・細胞診にて採取された微量腫瘍組織を用いてオルガノイドを樹立し、個々の患者ごとの腫瘍組織を用いた様々な解析が可能となるよう増幅・培養を行う。これを用いた解析結果と、臨床病理学的因子と合わせて比較検討することにより、個々の腫瘍に対する有効な治療法・新規の薬剤同定に結びつく知見を得ることを目的とする。","en":"In this study, we establish organoids using minimal tumor tissue obtained via biopsy or cytology. These organoids are then amplified and cultured to enable various analyses using tumor tissue from individual patients. By comparing the results of these analyses with clinical and pathological factors, we aim to gain insights that will lead to the identification of effective treatments and novel drugs for individual tumors."},"methods":{"ja":"・膵管がん17症例、膵管内乳頭粘液性腫瘍（IPMN）6症例の膵腫瘍組織から樹立したオルガノイドのWhole Exome sequencing、RNA sequencing、ATAC sequencing、ChIP sequencing、およびHi-C解析\n・膵がん11症例、IPMN由来の膵がん2症例、IPMN3症例、ならびに非腫瘍性疾患1症例それぞれから樹立したオルガノイドを対象としたLong read RNA-seq解析データ","en":"Whole Exome sequencing, RNA sequencing, ATAC sequencing, ChIP sequencing, and Hi-C analysis using organoids established from pancreatic cancer and Intraductal Papillary Mucinous Neoplasm (IPMN).\nLong read RNA-seq analysis using organoids established from pancreatic cancer, IPMN, IPMN-derived pancreatic cancers and Non-tumor pancreatic tissue."},"targets":{"ja":"膵管がん、IPMN、膵がん、IPMN由来の膵がん、非腫瘍性疾患","en":"Pancreatic ductal adenocarcinoma, IPMN, Pancreatic cancer, IPMN-derived pancreatic cancers , Non-tumor pancreatic tissue"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定\n発現\nクロマチン構造\nヒストン修飾","en":"Sequencing, Expression profiling, Chromatin structure profiling, Histone modification profiling"},"targets":{"ja":"膵管癌：17症例\n膵管内乳頭粘液性腫瘍：6＋3症例\n膵癌：11症例\n膵管内乳頭粘液性腫瘍（IPMN）由来の膵がん：2症例\n非腫瘍性疾患：1症例\n（日本人）","en":"Pancreatic ductal adenocarcinoma: 17 cases\nIntraductal papillary mucinous neoplasm (IPMN): 6+3 cases\nPancreatic cancer: 11 cases\nIPMN-derived pancreatic cancers: 2 cases\nNon-tumor pancreatic tissue: 1 case\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome、RNA-seq、ATAC-seq、ChIP-seq、Hi-C）","en":"NGS\n(Exome, RNA-seq, ATAC-seq, ChIP-seq, Hi-C)"}},"releaseNote":{"ja":"膵がん11症例、膵管内乳頭粘液性腫瘍（IPMN）由来の膵がん2症例、IPMN3症例、ならびに非腫瘍性疾患1症例それぞれから樹立したオルガノイド検体から抽出したRNAを用いたLong read RNA-seq解析データをfastqファイルにて提供する。","en":"RNAs extracted from organoids established from pancreatic cancer, intraductal papillary mucinous neoplasm (IPMN), IPMN-derived pancreatic cancers and Non-tumor pancreatic tissue were used for the Long read RNA sequencing analysis. Fastq files are provided."},"dataProviders":[{"name":{"ja":"立石 敬介","en":"Keisuke Tateishi"},"organization":{"name":{"ja":"東京大学医学部 消化器内科","en":"Department of Gastroenterology, The University of Tokyo"}}}],"researchProjects":[],"grants":[{"title":{"ja":"膵癌の包括的理解を目指した細胞間ネットワーク解析","en":"Comprehensive biological network analysis of pancreatic cancer"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["19K08435"]},{"title":{"ja":"クロマチン動態に基づいた膵癌の治療層別化・最適化","en":"Stratification and optimization of pancreatic cancer treatment based on chromatin dynamics"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["21K15966"]}],"relatedPublications":[{"title":"MNX1-HNF1B axis is indispensable for intraductal papillary mucinous neoplasm lineages","doi":"https://doi.org/10.1053/j.gastro.2021.12.254","datasets":["JGAD000365","JGAD000366","JGAD000367","E-GEAD-414","E-GEAD-415","E-GEAD-416","E-GEAD-417"]},{"title":"HNF1B-driven three-dimensional chromatin structure for molecular classification in pancreatic cancers","doi":"https://doi.org/10.1111/cas.15690","datasets":["JGAD000365","JGAD000366","JGAD000367","JGAD000633"]}],"datasets":["JGAD000366","E-GEAD-414","JGAD000367","E-GEAD-416","E-GEAD-417","JGAD000365","E-GEAD-415","JGAD000633","JGAD001103"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000366"]},{"principalInvestigator":{"ja":"山本 拓也","en":"Takuya Yamamoto"},"affiliation":{"ja":"プレシジョン免疫プロジェクト, 国立研究開発法人 医薬基盤・健康・栄養研究所","en":"Center for Intractable Diseases and ImmunoGenomics, National Institutes of Biomedical Innovation, Health and Nutrition"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"新規がん抗原の同定に基づく消化器がんおよび悪性黒色腫（メラノーマ）に対するワクチン開発基盤研究\r\n","en":"Basic research for vaccine development against gastrointestinal cancer and malignant melanoma based on the identification of novel cancer antigens\r\n"},"periodStart":"2024-11-12","periodEnd":"2027-03-31","datasets":["JGAD000366"]}]}