{"id":"hum0254","version":1,"url":"https://humandbs.dbcls.jp/research/hum0254/v1","datePublished":"2021-09-24","versions":[{"version":1,"datePublished":"2021-09-24"}],"title":{"ja":"血中セルフリーDNAからの配列決定ライブラリー調製法の開発","en":"Development of sequencing library preparation method from cell-free DNA in blood"},"summary":{"aims":{"ja":"新たに開発したアダプター連結反応を用いてセルフリーDNA（cell-free DNA：cfDNA）からライブラリー調製を実施するための条件検討","en":"Investigation of the conditions for library preparation from cell-free DNAs (cfDNAs) using the newly developed adapter linkage reaction."},"methods":{"ja":"新規技術で調製したcfDNAライブラリを用いて配列決定し、cfDNAの特性について解析を行う","en":"The cfDNA library prepared by the new technology was sequenced and analyzed for the properties of cfDNA."},"targets":{"ja":"健常人のヒト血漿由来cfDNA","en":"cfDNA from healthy human plasma"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"健常人：5名\n（日本人）","en":"5 healthy individuals\n(Japanese)"},"typeOfData":{"ja":"NGS\n（WGS）","en":"NGS\n(WGS)"}},"releaseNote":{"ja":"新たに開発した方法により、血漿中セルフリーDNAから効率よくライブラリを調製し、実施したwhole genome sequencing解析データをfastqファイルにて提供する。","en":"The cfDNA library prepared by the new technology is sequenced. Fastq files are provided."},"dataProviders":[{"name":{"ja":"三浦 史仁","en":"Fumihito Miura"},"organization":{"name":{"ja":"九州大学 大学院医学研究院 医化学分野","en":"Department of Biochemistry, Kyushu University Graduate School of Medical Sciences"}}}],"researchProjects":[],"grants":[{"title":{"ja":"先進メチローム解析の支援と高度化","en":"Advanced Methylomics: Support and Development"},"agency":{"ja":"日本医療研究開発機構（AMED） 創薬等ライフサイエンス研究支援基盤事業 創薬等先端技術支援基盤プラットフォーム（BINDS）","en":"Basis for Supporting Innovative Drug Discovery and Life Science Research (BINDS), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP20am0101103"]},{"title":{"ja":"次世代エピゲノム解析技術の開発とその応用","en":"Development and application of next-generation techniques for epigenome analysis"},"agency":{"ja":"科学研究費助成事業 新学術領域研究（研究領域提案型）","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)"},"grantIds":["17H06305"]},{"title":{"ja":"血漿セルフリーDNAの構造と修飾の網羅的解析","en":"Comprehensive analysis of structure and modifications of plasma cell-free DNA"},"agency":{"ja":"公益財団法人臨床研究奨励基金（2020年） 研究助成金","en":"Grant of The Clinical Research Promotion Foundation (2020)"},"grantIds":null}],"relatedPublications":[{"title":"Short single-stranded DNAs with putative non-canonical structures comprise a new class of plasma cell-free DNA.","doi":"https://doi.org/10.1186/s12915-021-01160-8","datasets":["JGAD000360"]}],"datasets":["JGAD000360"],"controlledAccessUsers":[]}