{"id":"hum0243","version":1,"url":"https://humandbs.dbcls.jp/research/hum0243/v1","datePublished":"2020-11-20","versions":[{"version":1,"datePublished":"2020-11-20"}],"title":{"ja":"ヒト化マウス（humanized mouse）の作製と免疫学研究への応用/ヒト・造血免疫系由来iPS細胞を用いた疾患と治療モデルの構築","en":"Development of humanized mice for human immunity research/Construction of disease and treatment model using iPS cells derived from human hematopoitec cells."},"summary":{"aims":{"ja":"ヒト免疫系を構築したヒト化マウスを用いた免疫細胞の機能の検証","en":"Evaluation of function of immue cells using humanized mice"},"methods":{"ja":"RNA sequencingやCap Analysis Gene Expression（CAGE）sequencingによる遺伝子発現解析","en":"RNA-seq or CAGE-seq"},"targets":{"ja":"ヒト急性骨髄性白血病116症例の患者末梢血もしくは骨髄液、患者検体を移植し白血病を発症したマウスの骨髄液もしくは脾臓細胞から得られたヒト白血病細胞（193サンプル）（RNA-seqは114症例166サンプル、CAGE-seqは27症例27サンプル、25症例が重複）、ならびに、臍帯血バンクに登録されている24検体から得られた細胞（44サンプル）","en":"RNAs extracted from cells derived from cord blood, and leukemia cells harvested from bone marrow aspirates or spleen cells of humanized mice generated from peripheral blood, bone marrow aspirates or samples from patients with Acute Myeloid Leukemia"},"url":{"ja":[{"url":"https://www.riken.jp/research/labs/ims/hum_dis_model/","text":"https://www.riken.jp/research/labs/ims/hum_dis_model/"}],"en":[{"url":"https://www.riken.jp/en/research/labs/ims/hum_dis_model/","text":"https://www.riken.jp/en/research/labs/ims/hum_dis_model/"}]}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"臍帯血：24名\n急性骨髄性白血病：116症例\n（日本人）","en":"Cord blood: 24 individuals\nAcute Myeloid Leukemia: 116 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq、CAGE-seq）","en":"NGS\n(RNA-seq, CAGE-seq)"}},"releaseNote":{"ja":"ヒト急性骨髄性白血病の患者末梢血もしくは骨髄液、患者検体を移植し白血病を発症したマウスの骨髄液もしくは脾臓細胞から得られたヒト白血病細胞、および臍帯血から得られた細胞から抽出したRNAを用いたRNA-seqまたはCAGE-seqデータをfastqおよびbedファイルにて提供する。","en":"RNAs extracted from cells derived from cord blood, and leukemia cells harvested from bone marrow aspirates or spleem cells of humanized mice generated from peripheral blood, bone marrow aspirates or samples from patients with Acute Myeloid Leukemia were used for RNA-seq or CAGE-seq analyses (fastq and bed files)."},"dataProviders":[{"name":{"ja":"石川 文彦","en":"Fumihiko Ishikawa"},"organization":{"name":{"ja":"理化学研究所統合生命医科学センター・ヒト疾患モデル研究チーム","en":"Laboratory for Human Disease Models, Riken Center for Integrative Medical Sciences"}}}],"researchProjects":[{"name":{"ja":"白血病再発克服プロジェクト","en":"Target AML project"},"url":{"ja":null,"en":null}}],"grants":[],"relatedPublications":[{"title":"Combined inhibition of XIAP and BCL2 drives maximal therapeutic efficacy in genetically diverse aggressive Acute Myeloid Leukemia","doi":"https://doi.org/10.1038/s43018-021-00177-w","datasets":["JGAD000356"]}],"datasets":["JGAD000356"],"controlledAccessUsers":[{"principalInvestigator":{"en":"Jinyan Huang"},"affiliation":{"en":"Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. Biomedical big data center, the First Affiliated Hospital, Zhejiang University"},"country":{"ja":"中国","en":"China"},"researchTitle":{"en":"Comprehensive analysis of alternative splicing in malignant tumors"},"periodStart":"2022-03-15","periodEnd":"2024-01-01","datasets":["JGAD000356"]},{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000356"]}]}