{"id":"hum0242","version":1,"url":"https://humandbs.dbcls.jp/research/hum0242/v1","datePublished":"2021-01-05","versions":[{"version":1,"datePublished":"2021-01-05"}],"title":{"ja":"変異遺伝子のモニタリングによる大腸癌術後の微少転移及び再発の新たな評価法の検証","en":"Frequent post-treatment monitoring of colorectal cancer using individualized ctDNA validated by multi-regional molecular profiling"},"summary":{"aims":{"ja":"大腸がん組織から同定された遺伝子変異を腫瘍由来血漿中循環DNA（ctDNA）としてモニタリングし、個別化腫瘍マーカーとしてのctDNAの臨床的妥当性を検証する","en":"To evaluate clinical validity of circulating tumor DNA (ctDNA) of tumor-unique mutations identified from colorectal cancer tissues, as a personalized tumor marker."},"methods":{"ja":"12症例（内、2症例には2つの大腸がんが観察された）から術前および術後の血漿、原発腫瘍、末梢血単核細胞（PBMC）を採取した。 原発腫瘍標本は手術直後に3箇所から採取し、すべての標本の細胞密度が顕微鏡で30％以上であることを確認した。42の原発腫瘍組織および12のPBMCからDNAを抽出し、HiSeq 2000 System（Illumina）を使用して、がん関連の151遺伝子についてtarget sequenceを実施した。","en":"Pre- and post-operational plasma, primary tumor, and peripheral blood mononuclear cells (PBMCs) were collected from 12 enrolled patients. Among these patients, two patients had two separate colorectal tumors. The primary tumor specimens were taken from three regions immediately after the operation, and the cellularity of all specimens was microscopically confirmed to be >30%. DNA was extracted from 42 primary tumor specimens and 12 PBMCs, and target sequencing using a HiSeq 2000 System (Illumina) conducted for 151 genes that have been implicated in a wide range of cancers was performed."},"targets":{"ja":"大腸がんで術前診断がStage III以上と診断された症例のうち書面での同意が得られた症例","en":"Colorectal cancer patients with preoperative diagnosis of Stage III or higher. Individual written consent was obtained from each participant."},"url":{"ja":[{"url":"https://nishizukalab.org/","text":"https://nishizukalab.org/"}],"en":[{"url":"https://nishizukalab.org/en/index.html","text":"https://nishizukalab.org/en/index.html"}]}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"大腸癌：12症例\n（日本人）","en":"12 colorectal cancer patients\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Target Capture）","en":"NGS\n(Target Capture)"}},"releaseNote":{"ja":"大腸がんStage III以上と診断された12症例の原発腫瘍組織およびPBMCから抽出したDNAを用いたTarget Capture解析結果をfastqおよびbamファイルにて提供する。","en":"DNAs extracted from primary tumor tissues and PBMC of 12 patients with colorectal cancer (Stage III or higher) were used for the target capture sequencing analysis (fastq and bam files)."},"dataProviders":[{"name":{"ja":"西塚 哲","en":"Satsoshi Nishizuka"},"organization":{"name":{"ja":"岩手医科大学 医歯薬総合研究所 医療開発研究部門","en":"Division of Biomedical Research and Development, Iwate Medical University Institute for Biomedical Sciences"}}}],"researchProjects":[],"grants":[{"title":{"ja":"薬剤耐性癌細胞の多様性に対応する至適分子標的薬選定プロセスの体系化","en":"Establishment of selection process of molecular targeting drugs in response to biological heterogeneity in drug-resistant cancer cells"},"agency":{"ja":"科学研究費助成事業 新学術領域研究（研究領域提案型）","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)"},"grantIds":["16H01578"]},{"title":{"ja":"薬剤耐性コロニーをモデルとした癌再発抑制へ繋がる化合物同定に関する研究（国際共同研究強化）","en":"Identification of compounds that potentially lead to the cancer relapse suppression on the basis of drug-resistant cancer subpopulation model (original)"},"agency":{"ja":"科学研究費助成事業 国際共同研究加速基金（国際共同研究強化）","en":"KAKENHI Fund for the Promotion of Joint International Research (Fostering Joint International Research)"},"grantIds":["15KK0317"]},{"title":{"ja":"Circulating tumor DNA検査の臨床導入における課題点の克服","en":"Circumvent practical issues for introducing ctDNA into daily practice"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["19K09224"]},{"title":{"ja":"Helicobacter pylori免疫応答が胃癌術後補助化学療法に及ぼす影響","en":"Effect of immune response to Helicobacter pylori on adjuvant chemotherapy for gastric cancer"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["19K09130"]},{"title":{"ja":"血漿中遊離変異DNA定量による食道癌モニタリングシステムの開発","en":"Development of monitoring system for esophageal cancer by quantifying circulating tumor DNA"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["17K10605"]},{"title":{"ja":"消化管癌のctDNAによる長期間モニタリングシステムの構築","en":"Development of longitudinal monitoring system for gastrointestinal cancer using circulating tumor DNA"},"agency":{"ja":"科学研究費助成事業 若手研究（B）","en":"KAKENHI Grant-in-Aid for Young Scientists (B)"},"grantIds":["18K15326"]},{"title":{"ja":"変異遺伝子のモニタリングによる大腸癌術後の微少転移及び再発の新たな評価法の検証","en":"The new estimation method of recurrence and metastasis on circulating tumor DNA detection in colorectal cancer patients"},"agency":{"ja":"科学研究費助成事業 若手研究（B）","en":"KAKENHI Grant-in-Aid for Young Scientists (B)"},"grantIds":["16K19951"]},{"title":{"ja":"血中遊離変異DNA解析を用いた食道がん患者におけるPrecision Medicineの開発","en":"Development of precision medicine for esophageal cancer patients using circulating tumor mutational DNA"},"agency":{"ja":"岩手医科大学圭陵会研究助成","en":"Keiryoukai Research Grant"},"grantIds":["136"]},{"title":{"ja":"大腸癌ctDNAに原発巣heterogeneityが及ぼす影響","en":"Impact of intratumor heterogeneity for ctDNA of colon cancer"},"agency":{"ja":"日本学術振興会藤田記念医学研究振興基金","en":"JSPS Fujita Memorial Fund for Medical Research"},"grantIds":null},{"title":{"ja":"進行がん治療の定量評価へ向けた高感度個別化腫瘍マーカーの開発","en":"Development of high-sensitive individualized tumor markers for quantitative evaluation of advanced cancer"},"agency":{"ja":"岩手県地域新成長産業創出促進事業費補助金","en":"Iwate Prefectural Regional Innovation Grant"},"grantIds":null}],"relatedPublications":[],"datasets":["JGAD000343"],"controlledAccessUsers":[]}