{"id":"hum0236","version":1,"url":"https://humandbs.dbcls.jp/research/hum0236/v1","datePublished":"2020-12-14","versions":[{"version":1,"datePublished":"2020-12-14"}],"title":{"ja":"肺がんの複雑な遺伝子変異を利用した腫瘍浸潤リンパ球療法の開発を目指した基礎的検討","en":"Development of tumor infiltrating lymphocytes therapy targeting gene mutation of lung cancer"},"summary":{"aims":{"ja":"非小細胞肺がんにおける異常スプライシングアイソフォームとネオ抗原候補の同定","en":"Identifying aberrant splicing isoforms and potential neoantigens in non-small cell lung cancer"},"methods":{"ja":"ロングリードシークエンサー（MinION）により、がんに発現する転写産物の全長を同定した。また、ショートリードシークエンサーによるRNA sequence、whole exome sequenceデータと組み合わせ、異常転写産物から生じるネオ抗原候補を同定した。","en":"Full-length cDNA sequencing using a long-read sequencer MinION, short-read RNA sequencing, and whole-exome sequencing were performed to detect aberrant splicing isoforms and neoantigens expressed in non-small cell lung cancer specimens."},"targets":{"ja":"非小細胞肺癌臨床検体（7例、非腫瘍組織と腫瘍組織のペア）","en":"Seven non-small cell lung cancer specimens (tumor and matched-non tumor pair)"},"url":{"ja":[{"url":"https://kero.hgc.jp/","text":"https://kero.hgc.jp/"}],"en":[{"url":"https://kero.hgc.jp/","text":"https://kero.hgc.jp/"}]}},"listingSummary":{"methods":{"ja":"配列決定\n発現","en":"Sequencing, Expression profiling"},"targets":{"ja":"非小細胞肺癌：7症例\n（日本人）","en":"7 non-small cell lung cancer patients\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome、RNA-seq）","en":"NGS\n(Exome, RNA-seq)"}},"releaseNote":{"ja":"非小細胞肺がん7症例の腫瘍組織とペア非腫瘍組織から抽出したDNA/RNAを用いたWhole Exome Sequencing解析並びにロングリード（MinION）とショートリード（HiSeq 2500）によるRNA-seq解析の結果をfastqファイルにて提供する。","en":"DNAs and RNAs extracted from clinical specimens (tumor and non-tumor tissues) of seven non-small cell lung cancer patients were used for the whole exome sequencing and RNA-seq (MinION/HiSeq 2500) analyses (fastq files)."},"dataProviders":[{"name":{"ja":"中面 哲也","en":"Tetsuya Nakatsura"},"organization":{"name":{"ja":"国立がん研究センター 先端医療開発センター 免疫療法開発分野","en":"Division of Cancer Immunotherapy, Exploratory Oncology Research and Clinical Trial Center, National Cancer Center"}}}],"researchProjects":[],"grants":[{"title":{"ja":"先進ゲノム解析研究推進プラットフォーム","en":"Platform for Advanced Genome Science"},"agency":{"ja":"科学研究費助成事業 新学術領域研究","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas"},"grantIds":["16H06279"]},{"title":{"ja":"ナノポアシークエンサーを用いたRNA合成及び分解の新規解析手法の開発","en":"Development of novel measurement methods for RNA synthesis and degradation using nanopore sequencing"},"agency":{"ja":"科学研究費助成事業 若手研究","en":"Grant-in-Aid for Early-Career Scientists"},"grantIds":["19K16108"]},{"title":{"ja":"長鎖シークエンサーによる全長RNA解読が可能にするがんの新規ネオ抗原評価法の開発","en":"Development of novel methods for detection of neoantigens in cancers by full-length transcriptome sequencing using nanopore sequencers"},"agency":{"ja":"科学研究費助成事業 若手研究","en":"Grant-in-Aid for Early-Career Scientists"},"grantIds":["19K16792"]}],"relatedPublications":[],"datasets":["JGAD000345"],"controlledAccessUsers":[{"principalInvestigator":{"en":"Youping Deng"},"affiliation":{"en":"Department of Complementary & Integrative Medicine, University of Hawaii Manoa"},"country":{"ja":"アメリカ合衆国 (ハワイ州)","en":"Hawaii, United States"},"researchTitle":{"ja":"Identification of driver genes and somatic mutations for lung cancer diagnosis and prognosis","en":"Identification of driver genes and somatic mutations for lung cancer diagnosis and prognosis"},"periodStart":"2018-10-04","periodEnd":"2029-07-10","datasets":["JGAD000345"]},{"principalInvestigator":{"ja":"河野 隆志","en":"Takashi Kohno"},"affiliation":{"ja":"ゲノム生物学研究分野, 国立研究開発法人国立がん研究センター研究所","en":"Division of Genome Biology, National Cancer Center Research Institute"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"AYA(Adolescence and Young Adult)世代がんの個別化予防に資する遺伝要因の同定を目指す研究","en":"Identification of genetic risk factors in AYA(Adolescence and Young Adult) cancer"},"periodStart":"2021-12-02","periodEnd":"2028-12-31","datasets":["JGAD000345"]},{"principalInvestigator":{"ja":"中岡 博史","en":"Hirofumi Nakaoka"},"affiliation":{"ja":"腫瘍ゲノム研究部, 公益財団法人佐々木研究所","en":"Department of Cancer Genome Research, Sasaki Institute"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"肺がん遺伝子解析研究","en":"Genetic analysis of lung cancer"},"periodStart":"2022-08-17","periodEnd":"2026-07-23","datasets":["JGAD000345"]},{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000345"]}]}