{"id":"hum0232","version":1,"url":"https://humandbs.dbcls.jp/research/hum0232/v1","datePublished":"2020-06-01","versions":[{"version":1,"datePublished":"2020-06-01"}],"title":{"ja":"胃癌由来循環腫瘍DNAモニタリングによる転移・再発の検証","en":"Evaluation of metastasis and recurrence by monitoring circulating tumor DNA of gastric cancer"},"summary":{"aims":{"ja":"胃がん組織から同定された遺伝子変異を腫瘍由来血漿中循環DNA（ctDNA）としてモニタリングし、個別化腫瘍マーカーとしてのctDNAの臨床的妥当性を検証する","en":"To evaluate a clinical validity of circulating tumor DNA (ctDNA) of tumor-unique mutations identified in gastric cancer tissues, as a personalized tumor marker."},"methods":{"ja":"登録された10症例から、術前および術後の血漿、原発腫瘍組織、末梢血単核細胞（PBMC）を採取した。 原発腫瘍標本は手術直後に3箇所から採取し、すべての標本の細胞密度が顕微鏡下で30％以上であることを確認した。 30の原発腫瘍組織および10のPBMCから抽出したDNAを用いた、がん関連151遺伝子のtarget capture sequencingを実施した。","en":"Pre- and post-operational plasma, primary tumor tissues, and peripheral blood mononuclear cells (PBMCs) were collected from 10 enrolled gastric cancer patients. The primary tumor specimens were taken from three regions immediately after the operation, and the cellularity of all specimens was microscopically confirmed to be >30%. DNAs are extracted from 30 primary tumor specimens and 10 PBMCs, and target capture sequencing for 151 genes that have been implicated in a wide range of cancers was performed."},"targets":{"ja":"胃がんの術前診断においてStage IB以上と診断された症例のうち、書面での同意が得られた10症例","en":"Gastric cancer patients with preoperative diagnosis of Stage IB or higher. An individual written consent was obtained from each participant."},"url":{"ja":[{"url":"https://nishizukalab.org/","text":"https://nishizukalab.org/"}],"en":[{"url":"https://nishizukalab.org/en/index.html","text":"https://nishizukalab.org/en/index.html"}]}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"胃癌：10症例\n（日本人）","en":"10 gastric cancer patients\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Target Capture）","en":"NGS\n(Target Capture)"}},"releaseNote":{"ja":"胃がんStage IB以上と診断された10症例の原発腫瘍組織およびPBMCから抽出したDNAを用いたTarget Capture解析結果をfastqおよびbamファイルにて提供する。","en":"DNAs extracted from primary tumor tissues and PBMC of 10 patients with gastric cancer (more than Stage IB) were used for the target capture sequencing analysis (fastq and bam files)."},"dataProviders":[{"name":{"ja":"西塚 哲","en":"Satsoshi Nishizuka"},"organization":{"name":{"ja":"岩手医科大学 医歯薬総合研究所 医療開発研究部門","en":"Division of Biomedical Research and Development, Iwate Medical University Institute for Biomedical Sciences"}}}],"researchProjects":[],"grants":[{"title":{"ja":"薬剤耐性癌細胞の多様性に対応する至適分子標的薬選定プロセスの体系化","en":"Establishment of selection process of molecular targeting drugs in response to biological heterogeneity in drug-resistant cancer cells"},"agency":{"ja":"科学研究費助成事業 新学術領域研究（研究領域提案型）","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)"},"grantIds":["16H01578"]},{"title":{"ja":"薬剤耐性コロニーをモデルとした癌再発抑制へ繋がる化合物同定に関する研究（国際共同研究強化）","en":"Identification of compounds that potentially lead to the cancer relapse suppression on the basis of drug-resistant cancer subpopulation model (original)"},"agency":{"ja":"科学研究費助成事業 国際共同研究加速基金（国際共同研究強化）","en":"KAKENHI Fund for the Promotion of Joint International Research (Fostering Joint International Research)"},"grantIds":["15KK0317"]},{"title":{"ja":"Circulating tumor DNA検査の臨床導入における課題点の克服","en":"Circumvent practical issues for introducing ctDNA into daily practice"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["19K09224"]},{"title":{"ja":"Helicobacter pylori免疫応答が胃癌術後補助化学療法に及ぼす影響","en":"Effect of immune response to Helicobacter pylori on adjuvant chemotherapy for gastric cancer"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["19K09130"]},{"title":{"ja":"血漿中遊離変異DNA定量による食道癌モニタリングシステムの開発","en":"Development of monitoring system for esophageal cancer by quantifying circulating tumor DNA"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["17K10605"]}],"relatedPublications":[{"title":"Analysis of mutational and proteomic heterogeneity of gastric cancer suggests an effective pipeline to monitor post-treatment tumor burden using circulating tumor DNA","doi":"https://doi.org/10.1371/journal.pone.0239966","datasets":["JGAD000325"]}],"datasets":["JGAD000325"],"controlledAccessUsers":[]}