{"id":"hum0220","version":1,"url":"https://humandbs.dbcls.jp/research/hum0220/v1","datePublished":"2020-06-02","versions":[{"version":1,"datePublished":"2020-06-02"}],"title":{"ja":"採血後の試験管保存状態が試験管内の化合物濃度に与える影響の研究","en":"A study of influence of storage condition after blood collection on the plasma/serum comcentrations of compounds"},"summary":{"aims":{"ja":"静脈採血から血漿凍結までの放置時間が、採血管内の血中タンパク質濃度に与える影響について探索する","en":"To clarify the stability of blood proteins during pre-analytical blood processing and storage, to compare the proteomes of similarly treated serum and plasma samples."},"methods":{"ja":"血漿・血清検体を用いて、プロテオーム解析を行う。採血してから遠心により血漿分離するまでの放置条件を振り、血漿と血清中のタンパク質濃度を比較する。得られた結果からタンパク質の濃度と放置時間の関係を検討し、経時的な濃度変化の目立つタンパク質を探索する。","en":"We compared plasma samples prepared by centrifugation after storage of blood at room temperature for 0, 15 or 30 minutes, or under refrigeration at 0-5°C for 1, 4 or 8 hours. We also compared protein levels between plasma and serum. The plasma and serum samples were digested by trypsin and analyzed by mass spectrometer."},"targets":{"ja":"健常者3名の血液（血漿、血清）","en":"Plasma and serum samples from heathy individuals"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"質量分析","en":"Mass spectrometry"},"targets":{"ja":"健常人：3名\n（日本人）","en":"3 heathy individuals\n(Japanese)"},"typeOfData":{"ja":"LC-MS/MS","en":"LC-MS/MS"}},"releaseNote":{"ja":"静脈血採血から血漿凍結までの放置条件が与える血漿・血清中タンパク質への影響を確認するため、質量分析計による測定を実施した結果を提供する（.wiff, .wiff.scan, .mgf, .txt）。","en":"The plasma samples were prepared by centrifugation after storage of blood at room temperature for 0, 15 or 30 minutes, or under refrigeration at 0-5°C for 1, 4 or 8 hours. The plasma and serum samples were analyzed by mass spectrometer (.wiff, .wiff.scan, .mgf and .txt files)."},"dataProviders":[{"name":{"ja":"大槻 純男","en":"Sumio Ohtsuki"},"organization":{"name":{"ja":"熊本大学大学院 生命科学研究部","en":"Faculty of Life Sciences, Kumamoto University"}}}],"researchProjects":[{"name":{"ja":"微生物薬学分野","en":"Laboratory of Pharmaceutical Microbiology"},"url":{"ja":[{"url":"https://ohtsuki-lab.jp/ja/","text":"https://ohtsuki-lab.jp/ja/"}],"en":[{"url":"https://ohtsuki-lab.jp/en/","text":"https://ohtsuki-lab.jp/en/"}]}}],"grants":[{"title":{"ja":"包括的メタボロミクス・ターゲットプロテオミクスによるがん診断・薬効診断マーカー探索と革新的統合臨床診断ネットワーク構築","en":"Cancer diagnosis/drug efficiency evaluation biomarker research by comprehensive metabolomics/targeted proteomics and establishment of innovative integrated clinical diagnosis network"},"agency":{"ja":"日本医療研究開発機構 革新的先端研究開発支援事業（AMED-CREST）","en":"Core Research and Evolutional Science and Technology, Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED-CREST)"},"grantIds":["JP19gm0710013"]}],"relatedPublications":[{"title":"Effects of Differences in Pre-Analytical Processing on Blood Protein Profiles Determined With SWATH-MS","doi":"https://doi.org/10.1016/j.jprot.2020.103824","datasets":["JGAD000314"]}],"datasets":["JGAD000314"],"controlledAccessUsers":[]}