{"id":"hum0218","version":1,"url":"https://humandbs.dbcls.jp/research/hum0218/v1","datePublished":"2020-10-09","versions":[{"version":1,"datePublished":"2020-10-09"}],"title":{"ja":"全身性強皮症患者の遺伝子発現解析","en":"Gene expression analysis in systemic sclerosis"},"summary":{"aims":{"ja":"全身性強皮症の病態に寄与する単球集団の解明","en":"To elucidate the subpopulation of monocytes that are related to the pathophysiology of systemic sclerosis"},"methods":{"ja":"末梢血から分取した単球のシングルセルRNA-seq","en":"Peripheral blood monocytes from 3 systemic sclerosis patients were collected and a single cell RNA-seq was performed for 984 cells."},"targets":{"ja":"全身性強皮症","en":"Systemic sclerosis"},"url":{"ja":[{"url":"https://ryumachi.umin.jp/","text":"https://ryumachi.umin.jp/"}],"en":[{"url":"https://www.h.u-tokyo.ac.jp/english/centers-services/clinical-divisions/allergy-and-rheumatology/index.html","text":"https://www.h.u-tokyo.ac.jp/english/centers-services/clinical-divisions/allergy-and-rheumatology/index.html"}]}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"全身性強皮症：3症例\n（日本人）","en":"Systemic sclerosis: 3 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq）","en":"NGS\n(scRNA-seq)"}},"releaseNote":{"ja":"全身性強皮症3症例の末梢血から分取した単球を単細胞へ分離した上でmRNAを抽出し、scRNA-seq解析（HiSeq）を実施した。解析した際の各遺伝子にマップされたリード数を提供する（txt）。","en":"Peripheral blood monocytes from 3 systemic sclerosis patients were collected and a single cell RNA-seq was performed for 984 cells (HiSeq). Read count data mapped to each gene are provided as txt files."},"dataProviders":[{"name":{"ja":"藤尾 圭志","en":"Keishi Fujio"},"organization":{"name":{"ja":"東京大学大学院 医学系研究科 アレルギー・リウマチ学","en":"Department of Allergy and Rheumatology, Graduate School of Medicine, The University of Tokyo"}}}],"researchProjects":[{"name":{"ja":"全身性強皮症患者の遺伝子発現解析","en":"Gene expression analysis of systemic sclerosis patients."},"url":{"ja":[{"url":"https://ryumachi.umin.jp/","text":"https://ryumachi.umin.jp/"}],"en":[{"url":"https://www.h.u-tokyo.ac.jp/english/centers-services/clinical-divisions/allergy-and-rheumatology/index.html","text":"https://www.h.u-tokyo.ac.jp/english/centers-services/clinical-divisions/allergy-and-rheumatology/index.html"}]}}],"grants":[{"title":{"ja":"Single-cell RNA-seqによる全身性強皮症の病原的免疫細胞の探索","en":"Single-cell RNA-seq inquiry into pathogenic immune cells in systemic sclerosis"},"agency":{"ja":"科学研究費助成事業 若手研究（B）","en":"KAKENHI Grant-in-Aid for Young Scientists (B)"},"grantIds":["17K16204"]}],"relatedPublications":[{"title":"Integrated bulk and single-cell RNA-sequencing identified disease-relevant monocytes and a gene network module underlying systemic sclerosis","doi":"https://doi.org/10.1016/j.jaut.2020.102547","datasets":["E-GEAD-344","JGAD000309"]}],"datasets":["E-GEAD-344"],"controlledAccessUsers":[]}