{"id":"hum0208","version":1,"url":"https://humandbs.dbcls.jp/research/hum0208/v1","datePublished":"2020-01-20","versions":[{"version":1,"datePublished":"2020-01-20"}],"title":{"ja":"頭頚部悪性腫瘍に対する網羅的遺伝子解析およびデータベースの構築","en":"Comprehensive genetic analysis and database construction for head and neck cancer"},"summary":{"aims":{"ja":"頭頸部悪性腫瘍症例の腫瘍組織および末梢血由来単核球を対象とした網羅的な遺伝子解析により、再発・転移予測マーカーの同定、新規分子標的治療法の確立を目指す。","en":"To clinically identify recurrence and metastasis prediction markers and establish new molecular target therapies, we conducted a conprehensive genetic analysis of head and neck malignant tumor tissues and patient-derived blood."},"methods":{"ja":"切除標本から診断に影響のない範囲で腫瘍組織を採取し、抽出したDNAを用いた全エクソームシーケンスを行った。正常組織として、末梢血単核球をコントロールとしている。","en":"DNA were extracted from tumor tissues and matched normal samples (peripheral blood mononuclear cells: PBMC), and subjected to whole exome sequencing analysis."},"targets":{"ja":"両側外耳道がんおよび片側外耳道がん症例","en":"Bilateral / unilateral cancer of the external auditory canal"},"url":{"ja":[{"url":"https://www.qent.med.kyushu-u.ac.jp/group/research.html","text":"https://www.qent.med.kyushu-u.ac.jp/group/research.html"}],"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"外耳道癌：10症例\n（日本人）","en":"10 patients with cancer of the external auditory canal\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome）","en":"NGS\n(Exome)"}},"releaseNote":{"ja":"両側外耳道がん1症例および片側外耳道がん9症例の腫瘍組織およびペア末梢血由来単核球（PBMC）から抽出したDNAを用いたExome解析の結果をbamファイルにて提供する。SureSelect Human All Exon V6 KitによりExon領域にしぼり、Illumina HiSeq X Tenにて平均長100塩基を解読した（Single-end）。","en":"DNAs extracted from tumor tissues and matched normal tissues (peripheral blood mononuclear cells) derived from 10 patients with cancer of the external auditory canal were used for the whole exome sequencing (bam files). Exons were narrowed down by using of SureSelect Human All Exon V6 Kit and read by Illumina HiSeq X Ten (Single-end: 100 bp)."},"dataProviders":[{"name":{"ja":"中川 尚志","en":"Takashi Nakagawa"},"organization":{"name":{"ja":"九州大学病院 耳鼻咽喉科・頭頸部外科","en":"Department of Otorhinolaryngology, Kyushu University"}}}],"researchProjects":[{"name":{"ja":"頭頸部腫瘍に対する網羅的ゲノム解析およびそのデータベースの構築","en":"Comprehensive genetic analysis and database construction for head and neck cancer"},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"側頭骨扁平上皮癌の網羅的組織解析に基づく新規統合的治療戦略の開発","en":"Development of novel synthesizing treatment strategy by exhaustive research on temporal bone squamous cell carcinoma"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["18H02951"]},{"title":{"ja":"側頭骨癌におけるインフラマゾーム関連分子の網羅的解析による炎症性発癌機構の解明","en":"Elucidation of inflammatory carcinogenesis by comprehensive analysis of inflammasome-related molecules of temporal bone cancer"},"agency":{"ja":"科学研究費助成事業 若手研究","en":"KAKENHI Grant-in-Aid for Young Scientists"},"grantIds":["18K16895"]}],"relatedPublications":[{"title":"Squamous cell carcinoma of the external auditory canal and middle ear: an operation combined with preoperative chemoradiotherapy and a free surgical margin.","doi":"https://doi.org/10.1097/01.mao.0000190463.88873.3d","datasets":null}],"datasets":["JGAD000300"],"controlledAccessUsers":[]}