{"id":"hum0207","version":1,"url":"https://humandbs.dbcls.jp/research/hum0207/v1","datePublished":"2020-01-06","versions":[{"version":1,"datePublished":"2020-01-06"}],"title":{"ja":"ヒト免疫細胞における遺伝子多型と遺伝子発現の関連解析","en":"Analysis of gene polymorphism and gene expression in human immune cells"},"summary":{"aims":{"ja":"滑膜線維芽細胞の炎症環境応答と関節リウマチの病態形成に対する寄与を解明する","en":"To elucidate the dynamics of synovial fibroblasts and its association with rheumatoid arthritis pathogenesis."},"methods":{"ja":"関節リウマチおよび変形性関節症（コントロール）患者由来の滑膜線維芽細胞を、関節内のサイトカイン（TNF-α, IL-1β, IL-6/sIL-6R, IL-17A, IL-18, IFN-γ, IFN-α, TGF-β1, 複合炎症をシミュレートした全8種のmixture [8-mix]）で24時間刺激した。また、同一患者より末梢血リンパ球5分画（CD4陽性T細胞、CD8陽性T細胞、B細胞、NK細胞、単球）を分取した。各サンプルを用いて、SNPアレイ・ChIP-seq（H3K27ac・H3K4me1・H3K4me3）・Hi-C・RNA-seq解析を実施した。","en":"Synovial fibroblasts from rheumatoid arthritis and osteoarthritis patients (as a control) were stimulated with 9 cytokine conditions (IFN-α, IFN-γ, TNF-α, IL-1β, IL-6/sIL-6R, IL-17, TGF-β1, IL-18 and the mixture of these 8 cytokines [8-mix] which simulate synergistic inflammatory environment in arthritic joints) for 24 hours. Five subsets of peripheral blood mononuclear cells (CD4+ T, CD8+ T, B, NK and monocytes) were collected from the same patients. The integrative analysis including expression quantitative trait loci (eQTL) was performed with genome, transcriptome and epigenome data."},"targets":{"ja":"関節リウマチ30症例、変形性関節症30症例","en":"Patients with rheumatoid arthritis or osteoarthritis"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現\nヒストン修飾\nクロマチン構造\neQTL","en":"Expression profiling, Histone modification profiling, Chromatin structure analysis, eQTL"},"targets":{"ja":"関節リウマチ：30症例\n変形性関節症：30症例\n（日本人）","en":"rheumatoid arthritis: 30 cases\nosteoarthritis: 30 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq、ChIP-seq、Hi-C）\neQTL","en":"NGS\n(RNA-seq, ChIP-seq, Hi-C)\neQTL"}},"releaseNote":{"ja":"関節リウマチ30症例および変形性膝関節症30症例（コントロール）由来の滑膜線維芽細胞を、関節内サイトカイン（TNF-α, IL-1β, IL-6/sIL-6R, IL-17A, IL-18, IFN-γ, IFN-α, TGF-β1, 複合炎症をシミュレートした全8種のmixture [8-mix]）により24時間刺激した。また、同一患者より末梢血リンパ球5分画（CD4陽性T細胞、CD8陽性T細胞、B細胞、NK細胞、単球）を分取した。各サンプルより抽出したDNA/RNAを用いたSNPアレイ・ChIP-seq（H3K27ac・H3K4me1・H3K4me3）・Hi-C・RNA-seq解析データのProcesssedデータおよび集計情報を提供する（txtファイル）。","en":"Synovial fibroblasts from 30 rheumatoid arthritis and 30 osteoarthritis patients were stimulated with 9 cytokine conditions (IFN-α, IFN-γ, TNF-α, IL-1β, IL-6/sIL-6R, IL-17, TGF-β1, IL-18 and the mixture of these 8 cytokines [8-mix] which simulate synergistic inflammatory environment in arthritic joints) for 24 hours. Five subsets of peripheral blood mononuclear cells (CD4+ T, CD8+ T, B, NK and monocytes) were collected from the patients. The integrative analysis including expression quantitative trait loci (eQTL) was performed with genome, transcriptome and epigenome data. Processed data and aggregated data are provided as text files."},"dataProviders":[{"name":{"ja":"藤尾 圭志","en":"Keishi Fujio"},"organization":{"name":{"ja":"東京大学大学院 医学系研究科 アレルギー・リウマチ学","en":"Department of Allergy and Rheumatology, Graduate School of Medicine, The University of Tokyo"}}}],"researchProjects":[{"name":{"ja":"ヒト免疫細胞における遺伝子多型と遺伝子発現の関連解析","en":"Association study between gene polymorphism and gene expression in human immune cells"},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"関節リウマチ滑膜線維芽細胞における炎症メディエーター産生エピゲノム機構の解明","en":"Analysis of epigenetic structure responsible for inflammatory mediator production from rheumatoid arthritis synovial fibroblast"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["18H02846"]},{"title":{"ja":"関節リウマチ検体のトランスクリプトーム解析で同定された新規シグナルの検討","en":"Analysis of signaling pathways identified by transcriptome analysis of rheumatoid arthritis samples"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["17K09972"]},{"title":{"ja":"統合疾患データベースの構築とその臨床応用","en":"Construction of integrated disease database and its clinical application"},"agency":{"ja":"共同研究費（武田薬品工業）","en":"Joint research fund (Takeda Pharmaceutical Company Limited)"},"grantIds":null},{"title":{"ja":"eQTLを基礎とした創薬標的の探索と治療法の開","en":"Search for drug targets and development of therapeutic methods based on eQTL"},"agency":{"ja":"共同研究費（武田薬品工業）","en":"Joint research fund (Takeda Pharmaceutical Company Limited)"},"grantIds":null}],"relatedPublications":[{"title":"Parsing multiomics landscape of activated synovial fibroblasts highlights drug targets linked to genetic risk of rheumatoid arthritis","doi":"https://doi.org/10.1136/annrheumdis-2020-218189","datasets":["NHA000134","NHA000132","NHA000133","NHA000135"]}],"datasets":["NHA000134","NHA000132","NHA000133","NHA000135"],"controlledAccessUsers":[]}