{"id":"hum0206","version":1,"url":"https://humandbs.dbcls.jp/research/hum0206/v1","datePublished":"2019-11-26","versions":[{"version":1,"datePublished":"2019-11-26"}],"title":{"ja":"妊娠関連疾患の胎盤栄養膜細胞株の樹立と細胞特性に関する研究","en":"Establishment and characterization of cell lines derived from pregnancy related diseases"},"summary":{"aims":{"ja":"胎盤異常を呈する全胞状奇胎（complete hydatidiform mole: CHM）の病態を解明するため、CHMより樹立したヒト胎盤栄養膜幹細胞（Trophoblastic stem cell：TS）の細胞特性を明らかにする","en":"A complete hydatidiform mole (CHM) is an abnormal pregnancy caused by genetic fertilization disorders. The aim of this study is to establish human trophoblast stem (TS) cell lines from CHMs and elucidate their pathogenesis."},"methods":{"ja":"遺伝子発現解析（RNA-seq）、メチル化解析（PBAT-seq）","en":"Transcriptome analysis (RNA-seq) , Methyloation analysis (PBAT-seq)"},"targets":{"ja":"CHM3症例の胎盤から単離した細胞性栄養膜細胞（Cytotrophoblast cell：CT）2検体、および、CT細胞から樹立したTS細胞株3検体","en":"Cytotrophoblast (CT) cells isolated from placenta tissues of CHMs and CHM-derived TS cells"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現\nメチル化","en":"Expression profiling, Methylation profiling"},"targets":{"ja":"全胞状奇胎：3症例\n（日本人）","en":"complete hydatidiform mole: 3 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（PBAT-seq、RNA-seq）","en":"NGS\n(PBAT-seq, RNA-seq)"}},"releaseNote":{"ja":"・WGBS\n全胞状奇胎（CHM）3症例の胎盤から単離した細胞性栄養膜細胞（Cytotrophoblast cell：CT）から樹立した胎盤栄養膜幹細胞（Trophoblastic stem cell：TS）から抽出したDNAを用いたPBAT-seq解析の結果をfastqファイルにて提供する。Post-Bisulfite Adaptor-Tagging（PBAT）法によるライブラリ作製後、HiSeq 1500/2500（Illumina）によりシークエンシングを行っている。\n・RNA-seq\nCHM3症例の胎盤から単離したCT細胞2検体、および、CTから樹立したTS細胞株3検体から抽出したRNAを用いたRNA-seq解析の結果をfastqファイルにて提供する。TruSeq Stranded mRNA LT Sample Prep Kitによるライブラリ作製後、HiSeq 2500（Illumina）によりシークエンシングを行っている。","en":"- PBAT-seq\nDNAs extracted from complete hydatidiform mole (CHM)-derived trophoblastic stem (TS) cells were used for PBAT-sequencing analysis (fastq format files). Libraries were prepared with Post-Bisulfite Adaptor-Tagging (PBAT) and sequenced using HiSeq 1500/2500 (Illumina).\n- RNA-seq\nRNAs extracted from cytotrophoblast (CT) cells isolated from placenta tissues of CHMs and CHM-derived TS cells were used for RNA-sequencing analysis (fastq format files). Libraries were prepared with TruSeq Stranded mRNA LT Sample Prep Kit and sequenced using HiSeq 2500 (Illumina)."},"dataProviders":[{"name":{"ja":"有馬 隆博","en":"Takahiro Arima"},"organization":{"name":{"ja":"東北大学大学院 医学系研究科 環境遺伝医学総合研究センター 情報遺伝学分野","en":"Department of Informative Genetics, Tohoku University Graduate School of Medicine"}}}],"researchProjects":[],"grants":[{"title":{"ja":"ヒト胎盤の発生・分化に関する理解と臓器チップモデルの作製","en":"Elucidation of the mechanisms underlying human placental development and design of a placenta-on-a-chip platform"},"agency":{"ja":"日本医療研究開発機構 革新的先端研究開発支援事業 ユニットタイプ（AMED-CREST）","en":"Core Research and Evolutional Science and Technology, Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED-CREST)"},"grantIds":["JP19gm1310001"]},{"title":{"ja":"生殖発生にかかわる細胞のエピゲノム解析基盤研究","en":"Epigenome analysis of cells in the placenta and endometrium forming the fetal-maternal interface"},"agency":{"ja":"日本医療研究開発機構 革新的先端研究開発支援事業 ユニットタイプ（AMED-CREST）","en":"Core Research and Evolutional Science and Technology, Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED-CREST)"},"grantIds":["JP17gm0510011"]}],"relatedPublications":[{"title":"Loss of p57KIP2 expression confers resistance to contact inhibition in human androgenetic trophoblast stem cells","doi":"https://doi.org/10.1073/pnas.1916019116","datasets":["JGAD000292"]}],"datasets":["JGAD000292"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000292"]}]}