{"id":"hum0199","version":1,"url":"https://humandbs.dbcls.jp/research/hum0199/v1","datePublished":"2020-06-29","versions":[{"version":1,"datePublished":"2020-06-29"}],"title":{"ja":"次世代シーケンサーを用いた泌尿器悪性腫瘍の治療標的分子の同定","en":"Identification of therapeutic target molecules in urological malignancies by using next generation sequencer"},"summary":{"aims":{"ja":"前立腺がんの発生、進行、治療耐性化における腫瘍組織内の遺伝子発現変化を明らかにする","en":"We analyzed gene expression changes in the development, progression and aquirement of therapeutic resistance of prostate cancer."},"methods":{"ja":"前立腺がんの腫瘍組織もしくは非腫瘍組織より抽出したtotal RNAを用いた次世代シークエンサーによるRNA-sequencing解析","en":"We extract total RNAs and performed RNA-sequence analysis."},"targets":{"ja":"手術の際に摘出した前立腺組織（腫瘍組織および非腫瘍組織）、治療耐性を獲得した症例の再発組織、転移組織","en":"Prostate tissues including benign and tumor tissues obtained by surgery and tumor tissues of metastasis or relapsed"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"前立腺癌：25症例\n（日本人）","en":"Prostate cancer: 25 cases\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"前立腺がん25症例の腫瘍組織もしくは非腫瘍組織から抽出したRNAを用いたRNA-Seq解析データをbamファイルにて提供する。TrueSeq RNA library preparation KitによるRNAライブラリ作製後、HiSeq 2500（Illumina）にて平均長50 bpを読んだ（Single-end）。","en":"RNAs were extracted from surgicallly resected tumor and non-tumor tissues of 25 prostate cancer patients. Libraries for RNA-seq were prepared with TrueSeq RNA library preparation Kit and RNA-seq was performed by using of HiSeq 2500 (Illumina) (50 bp, Single-end). RNA-seq data are provided as bam files."},"dataProviders":[{"name":{"ja":"藤村 哲也","en":"Tetsuya Fujimura"},"organization":{"name":{"ja":"東京大学医学部附属病院 泌尿器科","en":"Department of Urology, Graduate Scool of Medicine, University of Tokyo"}}}],"researchProjects":[{"name":{"ja":"次世代シーケンサーを用いた泌尿器悪性腫瘍における治療標的分子の同定","en":"Identification of therapeutic target molecules for urologic cancers by using next generation sequencer"},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"前立腺癌のホルモン療法抵抗性獲得に至るエピゲノム調節機構の統合的解明と臨床応用","en":"Investigation of epigenetic mechanisms driving resistance to hormone-therapy and their clinical implications in prostate cancer"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["17H04334"]},{"title":{"ja":"エストロゲン・アンドロゲンシグナルによる転移性前立腺癌のプレシジョンメディシン","en":"Precision medicine for metastatic prostate cancer via estrogen and androgen blockade"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["18K09128"]},{"title":{"ja":"Genetic scoreを用いた前立腺癌治療の個別化","en":"Personalized medicine for advanced prostate cancer"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["15K10576"]}],"relatedPublications":[{"title":"Identification of long non-coding RNAs in advanced prostate cancer associated with androgen receptor splicing factors","doi":"https://doi.org/10.1038/s42003-020-01120-y","datasets":["JGAD000283"]}],"datasets":["JGAD000283"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000283"]}]}