{"id":"hum0195","secondaryIds":[],"version":1,"url":"https://humandbs.dbcls.jp/research/hum0195/v1","datePublished":"2021-03-26","versions":[{"version":1,"datePublished":"2021-03-26"},{"version":2,"datePublished":"2022-03-02"},{"version":3,"datePublished":"2022-03-18"}],"title":{"ja":"消化器癌における遺伝子異常についての網羅的解析","en":"Comprehensive genetic analysis of gastrointestinal and hepatobiliary tumor"},"summary":{"aims":{"ja":"消化器がんの病態進展に関わる遺伝子異常を明らかにすること","en":"To elucidate the genetic changes in the progression of gastrointestinal and hepatobiliary tumors"},"methods":{"ja":"全ゲノム解析、全エクソン解析およびトランスクリプトーム解析","en":"whole genome sequencing, whole exome sequencing and RNA sequencing analysis"},"targets":{"ja":"結節内結節型肝がん5症例の腫瘍組織・非腫瘍組織・末梢血より単離したリンパ球\nC型肝炎ウイルス排除後に肝がんを発症した慢性肝炎/肝硬変症例、C型肝炎ウイルス陽性肝硬変/肝がん症例、および、C型肝炎ウイルス陰性肝がん症例","en":"nodule-in-nodule hepatocellular carcinoma\nliver cirrhosis and hepatocellular carcinoma after hepatitis C virus eradication, hepatitis C virus positive cirrhosis and hepatocellular carcinoma and hepatitis C virus negative hepatocellular carcinoma"},"url":{"ja":null,"en":null}},"listingSummary":{},"releaseNote":{"ja":"・結節内結節型肝がん5症例の腫瘍組織、非腫瘍組織、および末梢血より単離したリンパ球から抽出したDNAを用いたWGS解析の結果をfastqファイルにて提供する。\n・C型肝炎ウイルス排除後に肝がんを発症した慢性肝炎/肝硬変20症例、C型肝炎ウイルス陽性肝硬変/肝がん3症例、および、C型肝炎ウイルス陰性肝がん2症例の腫瘍組織、非腫瘍組織、および末梢血より単離したリンパ球から抽出したDNA/RNAを用いたExome、RNA-seq解析の結果をfastqファイルにて提供する。","en":"- DNAs extracted from tumor tissues, non-tumor tissues, and lymphocytes isolated from peripheral blood of 5 nodule-in-nodule hepatocellular carcinoma patients were used for whole genome sequencing analyses (fastq files).\n- DNAs/RNAs extracted from tumor tissues, non-tumor tissues, and lymphocytes isolated from peripheral blood of 20 liver cirrhosis and hepatocellular carcinoma after hepatitis C virus eradication, 3 hepatitis C virus positive cirrhosis and hepatocellular carcinoma and 2 hepatitis C virus negative hepatocellular carcinoma patients were used for whole exome sequencing and RNA sequencing analysis (fastq files)."},"dataProviders":[{"name":{"ja":"髙井 淳","en":"Atsushi Takai"},"organization":{"name":{"ja":"京都大学大学院 医学研究科 消化器内科学","en":"Gastroenterology and Hepatology, Graduate School of Medicine, Kyoto University"}}}],"researchProjects":[],"grants":[{"title":{"ja":"全ゲノム解析とオルガノイド培養を用いた多段階肝発癌分子メカニズムの解明","en":"Exploration for the molecular mechanism on multistep hepatocarcinogenesis using organoid culture system and whole genome sequencing analysis"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["19K08443"]},{"title":{"ja":"肝硬変\"再生結節\"の細胞クローン動態と発癌機構の解明","en":"Role of regeneration nodule in cirrhotic liver on tumorigenesis"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["17H04158"]}],"relatedPublications":[{"title":"Multiregional whole-genome sequencing of hepatocellular carcinoma with nodule-in-nodule appearance reveals stepwise cancer evolution","doi":"https://doi.org/10.1002/path.5533","datasets":["JGAD000273"]},{"title":"Oncogenic transcriptomic profile is sustained in the liver after the eradication of the hepatitis C virus","doi":"https://doi.org/10.1093/carcin/bgab014","datasets":["JGAD000332"]},{"title":"DNA methyltransferase 3B plays a protective role against hepatocarcinogenesis caused by chronic inflammation via maintaining mitochondrial homeostasis","doi":"https://doi.org/10.1038/s41598-020-78151-2","datasets":["JGAD000332"]}],"datasets":["JGAD000273","JGAD000331","JGAD000332"],"controlledAccessUsers":[{"principalInvestigator":{"en":"Jinyan Huang"},"affiliation":{"en":"Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. Biomedical big data center, the First Affiliated Hospital, Zhejiang University"},"country":{"ja":"中国","en":"China"},"researchTitle":{"en":"Comprehensive analysis of alternative splicing in malignant tumors"},"periodStart":"2022-03-15","periodEnd":"2024-01-01","datasets":["JGAD000273","JGAD000331"]},{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000273","JGAD000331","JGAD000332","JGAD000375","JGAD000629"]}]}