{"id":"hum0195","version":3,"url":"https://humandbs.dbcls.jp/research/hum0195/v3","datePublished":"2022-03-18","versions":[{"version":1,"datePublished":"2021-03-26"},{"version":2,"datePublished":"2022-03-02"},{"version":3,"datePublished":"2022-03-18"}],"title":{"ja":"消化器癌における遺伝子異常についての網羅的解析","en":"Comprehensive genetic analysis of gastrointestinal and hepatobiliary tumor"},"summary":{"aims":{"ja":"消化器がんの病態進展に関わる遺伝子異常を明らかにすること","en":"To elucidate the genetic changes in the progression of gastrointestinal and hepatobiliary tumors"},"methods":{"ja":"全ゲノム解析、全エクソン解析、トランスクリプトーム解析、および、targeted deep sequencing解析","en":"whole genome sequencing, whole exome sequencing, RNA sequencing, and targeted deep sequencing analysis"},"targets":{"ja":"・結節内結節型肝がん症例の腫瘍組織・非腫瘍組織・末梢血より単離したリンパ球\n・C型肝炎ウイルス排除後に肝がんを発症した慢性肝炎/肝硬変症例、C型肝炎ウイルス陽性肝硬変/肝がん症例、および、C型肝炎ウイルス陰性肝がん症例\n・胃がん症例、胃粘膜下腫瘍症例","en":"- nodule-in-nodule hepatocellular carcinoma\n- liver cirrhosis and hepatocellular carcinoma after hepatitis C virus eradication, hepatitis C virus-positive cirrhosis and hepatocellular carcinoma, and hepatitis C virus-negative hepatocellular carcinoma\n- gastric cancer and gastric submucosal tumor"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定\n発現","en":"Sequencing, Expression profiling"},"targets":{"ja":"結節内結節型肝癌：5症例\nC型肝炎ウイルス排除後に肝癌を発症した慢性肝炎/肝硬変：20症例\nC型肝炎ウイルス陽性肝硬変/肝癌：3症例\nC型肝炎ウイルス陰性肝癌：2症例\n胃癌：42 ＋ 1症例、胃粘膜下腫瘍：3症例\n（日本人）","en":"5 nodule-in-nodule hepatocellular carcinomas\n20 liver cirrhosis and hepatocellular carcinomas after hepatitis C virus eradication\n3 hepatitis C virus positive cirrhosis and hepatocellular carcinomas\n2 hepatitis C virus negative hepatocellular carcinomas\n42 + 1 patients with gastric cancer and 3 patients with gastric submucosal tumor\n(Japanese)"},"typeOfData":{"ja":"NGS\n（WGS、Exome、RNA-seq、Target Capture）","en":"NGS\n(WGS, Exome, RNA-seq, Target Capture)"}},"releaseNote":{"ja":"胃がん1症例のマルチサンプル（胃がん、腺腫、化生、胃底腺ポリープ、正常胃組織、末梢血、各1検体）から抽出したDNAを用いたWhole exome sequencing解析データをfastqファイルにて提供する。","en":"DNAs extracted from tumor tissues, non-tumor tissues, and peripheral blood samples of 1 patients with gastric cancer were used for whole exome sequencing (fastq files)."},"dataProviders":[{"name":{"ja":"髙井 淳","en":"Atsushi Takai"},"organization":{"name":{"ja":"京都大学大学院 医学研究科 消化器内科学","en":"Gastroenterology and Hepatology, Graduate School of Medicine, Kyoto University"}}}],"researchProjects":[],"grants":[{"title":{"ja":"全ゲノム解析とオルガノイド培養を用いた多段階肝発癌分子メカニズムの解明","en":"Exploration for the molecular mechanism on multistep hepatocarcinogenesis using organoid culture system and whole genome sequencing analysis"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["19K08443"]},{"title":{"ja":"肝硬変\"再生結節\"の細胞クローン動態と発癌機構の解明","en":"Role of regeneration nodule in cirrhotic liver on tumorigenesis"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["17H04158"]}],"relatedPublications":[{"title":"Multiregional whole-genome sequencing of hepatocellular carcinoma with nodule-in-nodule appearance reveals stepwise cancer evolution","doi":"https://doi.org/10.1002/path.5533","datasets":["JGAD000273"]},{"title":"Oncogenic transcriptomic profile is sustained in the liver after the eradication of the hepatitis C virus","doi":"https://doi.org/10.1093/carcin/bgab014","datasets":["JGAD000332"]},{"title":"DNA methyltransferase 3B plays a protective role against hepatocarcinogenesis caused by chronic inflammation via maintaining mitochondrial homeostasis","doi":"https://doi.org/10.1038/s41598-020-78151-2","datasets":["JGAD000332"]},{"title":"Expansion of gastric intestinal metaplasia with copy number aberrations contributes to field cancerization","doi":"https://doi.org/10.1158/0008-5472.CAN-21-1523","datasets":["JGAD000375"]}],"datasets":["JGAD000273","JGAD000331","JGAD000375","JGAD000629","JGAD000332"],"controlledAccessUsers":[{"principalInvestigator":{"en":"Jinyan Huang"},"affiliation":{"en":"Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. Biomedical big data center, the First Affiliated Hospital, Zhejiang University"},"country":{"ja":"中国","en":"China"},"researchTitle":{"en":"Comprehensive analysis of alternative splicing in malignant tumors"},"periodStart":"2022-03-15","periodEnd":"2024-01-01","datasets":["JGAD000273","JGAD000331"]},{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000273","JGAD000331","JGAD000332","JGAD000375","JGAD000629"]}]}