{"id":"hum0188","version":1,"url":"https://humandbs.dbcls.jp/research/hum0188/v1","datePublished":"2019-06-28","versions":[{"version":1,"datePublished":"2019-06-28"}],"title":{"ja":"胃がん細胞株のゲノム解析による治療標的同定","en":"Exploration for molecular targets in gastric cancer cell lines"},"summary":{"aims":{"ja":"胃がん細胞株のゲノム解析による治療標的同定","en":"Screening of therapeutic targets in gastric cancer cell lines"},"methods":{"ja":"Whole Exome sequecingおよびRNA-seq","en":"Whole Exome sequecing and RNA-seq"},"targets":{"ja":"胃がん細胞株OCUM-8, OCUM-9","en":"Gastric cancer cell lines, OCUM-8 and OCUM-9"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定\n発現","en":"Sequencing, Expression profiling"},"targets":{"ja":"胃癌細胞株：2検体\n（細胞株）","en":"2 gastric cancer cell lines\n(Cell-line)"},"typeOfData":{"ja":"NGS\n（Exome、RNA-seq）","en":"NGS\n(Exome, RNA-seq)"}},"releaseNote":{"ja":"胃がん細胞株（OCUM-8、OCUM-9）から抽出したDNAおよびRNAを用いたWhole Exome sequecingデータおよびRNA-seqデータをfastqおよびbamファイルにて提供する。ゲノムDNAよりエクソン領域をSureSelect Human All Exon V5で濃縮しHiSeq 2500で解析した。また純化したRNAをNEBNext Ultra Directional RNA Library Prep Kitによりライブラリー調整し、HiSeq 2500で解析した。","en":"Genomic DNA and total RNA were isolated from each cell line (OCUM-8, OCUM-9). DNA was subjected to enrichment of exonic fragments with a SureSelect Human All Exon V5 followed by nucleotide sequencing with the HiSeq 2500 platform using the paired-end option. Total RNA was subjected to RNA-seq with HiSeq 2500 using a NEBNext Ultra Directional RNA Library Prep Kit. Data are provided as fastq and bam files."},"dataProviders":[{"name":{"ja":"間野 博行","en":"Hiroyuki Mano"},"organization":{"name":{"ja":"国立がん研究センター研究所細胞情報学分野","en":"Division of Cellular Signaling, National Cancer Center Research Institute"}}}],"researchProjects":[],"grants":[{"title":{"ja":"がん治療標的探索プロジェクト","en":"Project for Exploration of Therapeutic Targets in Cancer"},"agency":{"ja":"日本医療研究開発機構（AMED） 革新的先端研究開発支援事業 インキュベートタイプ（LEAP）","en":"Leading Advanced Projects for medical innovation (LEAP), Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP17am0001001"]}],"relatedPublications":[{"title":"Identification of candidates for driver oncogenes in scirrhous-type gastric cancer cell lines.","doi":"https://doi.org/10.1111/cas.14111","datasets":["JGAD000260"]}],"datasets":["JGAD000260"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000260"]}]}