{"id":"hum0181","version":1,"url":"https://humandbs.dbcls.jp/research/hum0181/v1","datePublished":"2019-09-25","versions":[{"version":1,"datePublished":"2019-09-25"}],"title":{"ja":"副腎皮質におけるアルドステロン産生の老化の解明","en":"Aging of steroid production in the adrenal bland."},"summary":{"aims":{"ja":"原発性アルドステロン症（Primary Aldosteronism; PA）は二次性高血圧の原因として最も頻度が高い疾患である。これまでに、アルドステロン（Aldosterone; ALDO）合成酵素（CYP11B2）の免疫組織化学染色により、ALDO合成が従来考えられてきた球状層だけでなく、副腎皮質嚢下のCYP11B2が強発現する結節状構造(Aldosterone producing cell cluster; APCC 新規命名)においても行われることを報告した1。APCCの数と大きさは50歳までは年齢とともに増加する2。本研究では、50歳以上の症例におけるAPCCの数と大きさの変化について検討する。（1：J Clin Endocrinol Metab 2010;95,2296-305、2：Int J Endocrinol 2016;7834356）","en":"Immunohistochemistry of human aldosterone synthase (CYP11B2) has revealed that most of aldosterone is autonomously produced in aldosterone-producing cell clusters (APCCs) beneath the capsule of adult adrenals rather than physiologically in the zona glomerulosa (ZG)¹. APCCs have been occasionally found to harbor a somatic mutation of ion channel/pump genes, and number and size of APCCs increase with age until 50 years old², were reported so far. In this study, we aimed to study if APCCs are increased in individuals older than 50 years where the reninangiotensin system (RAS) is generally suppressed due to aging.\n1: J Clin Endocrinol Metab 2010;95,2296-305, 2: Int J Endocrinol 2016;7834356"},"methods":{"ja":"50歳以上の剖検症例（50-103歳、85症例）の副腎検体を用いて、CYP11B2と3-beta-hydroxysteroid dehydrogenase (HSD3B2) の免疫組織化学染色を行い、切片あたりのAPCCの面積の割合（APCC area per whole adrenal area [AA / WAA]、％）および切片の面積当たりのAPCCの数（number of APCC [NOA] / WAA）を算出して統計学的解析を行った。","en":"Archival adrenal gland were immunohistochemically analyzed using antibodies for aldosterone-synthase (CYP11B2) and 3β-hydroxysteroid dehydrogenase (3β-HSD). APCC areas per whole adrenal area (AA / WAA) and number of APCC per whole adrenal area (NOA / WAA) were measured and used for statistical analyses."},"targets":{"ja":"埼玉医科大学病院において剖検を行った50歳以上の症例","en":"85 anatomical individuals in the Saitama Medical University Hospital, who died at ages from 50 to 103 years."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"病理画像","en":"Immunohistochemistry"},"targets":{"ja":"病理解剖を施行された50歳以上の症例：85症例\n（日本人）","en":"85 anatomical cases who were more than 50 years old\n(Japanese)"},"typeOfData":{"ja":"病理スライドガラス画像","en":"Pathology slide images"}},"releaseNote":{"ja":"何らかの疾患により副腎の病理解剖を受けた50歳以上（50-103歳）の85名を対象にCYP11B2・HSD3B2・HE免疫組織化学染色を実施した際の病理スライドガラス画像をsvs形式ファイルにて提供する。","en":"Archival adrenal gland were immunohistochemically analyzed using antibodies for aldosterone-synthase (CYP11B2) and 3β-hydroxysteroid dehydrogenase (3β-HSD). Histological data (immunostaining for CYP11B2, 3β-HSD, hematoxylin and eosin staining) were provided (svs files)."},"dataProviders":[{"name":{"ja":"西本 紘嗣郎","en":"Koshiro Nishimoto"},"organization":{"name":{"ja":"埼玉医科大学国際医療センター 泌尿器腫瘍科","en":"Saitama Medical University International Medical Center, Department of UroOncology"}}}],"researchProjects":[],"grants":[{"title":{"ja":"体細胞変異に着目したアルドステロン産生細胞クラスターの病変進展メカニズムの解明","en":"Elucidation of progression from aldosterone-producing cell cluster leading to aldosterone-producing adenoma"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["15K10650"]},{"title":{"ja":"ステロイド産生能に着目した原発性アルドステロン症の新規治療戦略","en":"Novel therapeutic strategies for primary aldosteronism by focusing on steroidogenesis"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["18K09205"]},{"title":{"ja":"体細胞モザイシズムを呈する原発性アルドステロン症の病変・病型形成機構の解明","en":"Mechanisms for development of culprit lesions in primary aldosteronism that exhibits somatic mosaicism"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["17K09890"]},{"title":{"ja":"アルドステロン産生腺腫発生の分子基盤解明"},"agency":{"ja":"公益財団法人山口内分泌疾患研究振興財団研究助成金","en":"Yamaguchi Endocrine Research Foundation Grant"},"grantIds":null},{"title":{"ja":"アルドステロン産生腺腫に判明したイオンチャネル・ポンプ遺伝子体細胞変異が腺腫形成に関与する分子基盤の解明"},"agency":{"ja":"冲中記念成人病研究所「研究助成」","en":"Okinaka Memorial Institute for Medical Research Grant"},"grantIds":null}],"relatedPublications":[{"title":"Expression of aldosterone synthase cyp11b2 was inversely correlated with longevity.","doi":"https://doi.org/10.1016/j.jsbmb.2019.04.010","datasets":null},{"title":"Development of monoclonal antibodies against the human 3beta-hydroxysteroid dehydrogenase/isomerase isozymes.","doi":"https://doi.org/10.1016/j.steroids.2017.08.011","datasets":null}],"datasets":["NHA000087"],"controlledAccessUsers":[]}