{"id":"hum0161","version":2,"url":"https://humandbs.dbcls.jp/research/hum0161/v2","datePublished":"2022-12-27","versions":[{"version":1,"datePublished":"2021-06-04"},{"version":2,"datePublished":"2022-12-27"}],"title":{"ja":"シークエンス解析によるがんゲノム研究：肝芽腫","en":"Genome sequencing analysis for hepatoblastoma"},"summary":{"aims":{"ja":"日本人の肝芽腫におけるゲノム変異を探索する","en":"To investigate genomic alterations of Japanese hepatoblastomas"},"methods":{"ja":"肝芽腫切除切片における非腫瘍組織（正常組織）および腫瘍組織より抽出したDNAを使用したNGSライブラリーを作成後、Illumina HiSeq 2500にて塩基配列を決定する。","en":"DNAs were extracted from hepatoblastoma tissues and paired non-cancer (normal) tissues. NGS libaraies were prepared by using TruSeq Nano DNA Low Throughput Library Prep Kit for whole genome sequencing (WGS). Sequencing was perfomred by Illumina HiSeq 2500."},"targets":{"ja":"肝芽腫患者の非腫瘍組織および腫瘍組織のDNA","en":"DNAs extracted from cancer tissues and non-cancer tissues of Japanese hepatoblastoma patients."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"肝芽腫：33症例\n（日本人）","en":"33 hepatoblastomas\n(Japanese)"},"typeOfData":{"ja":"NGS\n（WGS）","en":"NGS\n(WGS)"}},"releaseNote":{"ja":"JGAD000234を特定の解析パイプラインにより加工したアライメント結果 [CRAM]、サンプルごとのバリアントコール結果 [gVCF]、データセットごとのバリアントコール結果 [aggregated VCF] を提供する。利用を希望する場合は、データ利用申請書にJGAD000234（元データ）とJGAD000688（加工データ）を記載すること。","en":"The alignment results [CRAM], variant call results per sample [gVCF], and variant call results per dataset [aggregated VCF] processed JGAD000234 in a certain workflow were provided. If you plan to use the data, please indicate both original data (JGAD000234) and processed data (JGAD000688) on the application form for data use."},"dataProviders":[{"name":{"ja":"中川 英刀","en":"Hidewaki Nakagawa"},"organization":{"name":{"ja":"理化学研究所 生命医科学研究センター","en":"RIKEN Center for Integrative Medical Sciences"}}}],"researchProjects":[],"grants":[],"relatedPublications":[],"datasets":["JGAD000234","JGAD000688","JGAD000889"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"有田 正規","en":"Masanori Arita"},"affiliation":{"ja":"生命情報・DDBJ センター, 国立遺伝学研究所","en":"Bioinformation and DDBJ Center, National Institute of Genetics"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"DDBJ センターと NBDC による JGA 解析データの提供","en":"Provision of processed JGA data analyzed by DDBJ Center and NBDC"},"periodStart":"2021-10-07","periodEnd":"2030-03-31","datasets":["JGAD000234"]}]}