{"id":"hum0161","version":1,"url":"https://humandbs.dbcls.jp/research/hum0161/v1","datePublished":"2021-06-04","versions":[{"version":1,"datePublished":"2021-06-04"},{"version":2,"datePublished":"2022-12-27"}],"title":{"ja":"シークエンス解析によるがんゲノム研究：肝芽腫","en":"Genome sequencing analysis for hepatoblastoma"},"summary":{"aims":{"ja":"日本人の肝芽腫におけるゲノム変異を探索する","en":"To investigate genomic alterations of Japanese hepatoblastomas"},"methods":{"ja":"肝芽腫切除切片における非腫瘍組織（正常組織）および腫瘍組織より抽出したDNAを使用したNGSライブラリーを作成後、Illumina HiSeq 2500にて塩基配列を決定する。","en":"DNAs were extracted from hepatoblastoma tissues and paired non-cancer (normal) tissues. NGS libaraies were prepared by using TruSeq Nano DNA Low Throughput Library Prep Kit for whole genome sequencing (WGS). Sequencing was perfomred by Illumina HiSeq 2500."},"targets":{"ja":"肝芽腫患者の非腫瘍組織および腫瘍組織のDNA","en":"DNAs extracted from cancer tissues and non-cancer tissues of Japanese hepatoblastoma patients."},"url":{"ja":null,"en":null}},"listingSummary":{},"releaseNote":{"ja":"肝芽腫切除切片の非腫瘍組織（正常組織）および腫瘍組織から抽出したDNAを使用したWGS解析の結果（HiSeq 2500）をFastqファイルで提供する（Paired-end）。","en":"DNAs extracted from cancer tissues and paired non-cancer (normal) tissues obtained from hepatoblastoma patients were used for WGS (HiSeq 2500). Fastq files are provided (Paired-end)."},"dataProviders":[{"name":{"ja":"中川 英刀","en":"Hidewaki Nakagawa"},"organization":{"name":{"ja":"理化学研究所 生命医科学研究センター","en":"RIKEN Center for Integrative Medical Sciences"}}}],"researchProjects":[],"grants":[],"relatedPublications":[],"datasets":["JGAD000234","JGAD000688","JGAD000889"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"有田 正規","en":"Masanori Arita"},"affiliation":{"ja":"生命情報・DDBJ センター, 国立遺伝学研究所","en":"Bioinformation and DDBJ Center, National Institute of Genetics"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"DDBJ センターと NBDC による JGA 解析データの提供","en":"Provision of processed JGA data analyzed by DDBJ Center and NBDC"},"periodStart":"2021-10-07","periodEnd":"2030-03-31","datasets":["JGAD000234"]}]}