{"id":"hum0160","version":1,"url":"https://humandbs.dbcls.jp/research/hum0160/v1","datePublished":"2019-05-28","versions":[{"version":1,"datePublished":"2019-05-28"},{"version":2,"datePublished":"2021-07-13"}],"title":{"ja":"シークエンス解析によるがんゲノム研究：食道がん","en":"Genome sequencing analysis for esophageal squamous cell carcinoma"},"summary":{"aims":{"ja":"日本人の食道がんにおけるゲノム変異を探索する","en":"To investigate genomic alterations of Japanese esophageal squamous cell carcinomas"},"methods":{"ja":"食道がん切除切片における非腫瘍組織（正常組織）および腫瘍組織より抽出したDNAを使用したNGSライブラリーを作成後、Illumina HiSeq 2000/2500/X Fiveにて塩基配列を決定する。","en":"DNAs were extracted from esophageal squamous cell carcinoma tissues and paired non-cancer (normal) tissues. NGS libaraies were prepared by using TruSeq Nano DNA Low Throughput Library Prep Kit for whole genome sequencing (WGS). Sequencing was perfomred by Illumina HiSeq 2000/2500/X Five."},"targets":{"ja":"食道がん患者の非腫瘍組織および腫瘍組織のDNA","en":"DNAs extracted from cancer tissues and non-cancer tissues of Japanese esophageal squamous cell carcinoma patients."},"url":{"ja":null,"en":null}},"listingSummary":{},"releaseNote":{"ja":"食道がん切除切片の非腫瘍組織（正常組織）および腫瘍組織から抽出したDNAを使用したWGS解析の結果（HiSeq 2000/2500/X Five）をFastqファイルで提供する（Paired-end）。","en":"DNAs extracted from cancer tissues and paired non-cancer (normal) tissues obtained from esophageal squamous cell carcinoma patients were used for WGS (HiSeq 2000/2500/X Five). Fastq files are provided (Paired-end)."},"dataProviders":[{"name":{"ja":"中川 英刀","en":"Hidewaki Nakagawa"},"organization":{"name":{"ja":"理化学研究所 生命医科学研究センター","en":"RIKEN Center for Integrative Medical Sciences"}}}],"researchProjects":[],"grants":[],"relatedPublications":[],"datasets":["JGAD000233","JGAD000405"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"山本 雅之","en":"Masayuki Yamamoto"},"affiliation":{"ja":"東北メディカル・メガバンク機構, 東北大学","en":"Tohoku Medical Megabank Organization"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"日本人全ゲノムデータベースの構築","en":"Construction of Japanese whole genome database"},"periodStart":"2019-06-24","periodEnd":"2022-04-15","datasets":["JGAD000233"]},{"principalInvestigator":{"ja":"白石 航也","en":"Kouya Shiraishi"},"affiliation":{"ja":"ゲノム生物学研究分野, 国立研究開発法人国立がん研究センター研究所","en":"Division of Genome Biology, National Cancer Research Institute"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"ゲノム解析に基づく宿主並びに腫瘍における免疫応答ネットワーク機構の解明","en":"Elucidation of immune-system networks between host and tumor based on genomic analysis"},"periodStart":"2019-08-05","periodEnd":"2029-03-31","datasets":["JGAD000233"]}]}