{"id":"hum0159","version":2,"url":"https://humandbs.dbcls.jp/research/hum0159/v2","datePublished":"2022-12-27","versions":[{"version":1,"datePublished":"2021-06-01"},{"version":2,"datePublished":"2022-12-27"}],"title":{"ja":"シークエンス解析によるがんゲノム研究：大腸がん","en":"Genome sequencing analysis for colorectal cancer"},"summary":{"aims":{"ja":"日本人の大腸がんにおけるゲノム変異を探索する","en":"To investigate genomic alterations of Japanese colorectal cancers"},"methods":{"ja":"大腸がん切除切片における非腫瘍組織（正常組織）および腫瘍組織より抽出したDNAを使用したNGSライブラリーを作成後、Illumina HiSeq 2000にて塩基配列を決定する。","en":"DNAs were extracted from colorectal cancer tissues and paired non-cancer (normal) tissues. NGS libaraies were prepared by using TruSeq DNA LT Sample Prep Kit or TruSeq Nano DNA Low Throughput Library Prep Kit for whole genome sequencing (WGS). Sequencing was perfomred by Illumina HiSeq 2000."},"targets":{"ja":"大腸がん患者の非腫瘍組織および腫瘍組織のDNA","en":"DNAs extracted from cancer tissues and non-cancer tissues of Japanese colorectal cancer patients."},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"大腸癌：10症例\n（日本人）","en":"10 colorectal cancers\n(Japanese)"},"typeOfData":{"ja":"NGS\n（WGS）","en":"NGS\n(WGS)"}},"releaseNote":{"ja":"JGAD000235を特定の解析パイプラインにより加工したアライメント結果 [CRAM]、サンプルごとのバリアントコール結果 [gVCF]、データセットごとのバリアントコール結果 [aggregated VCF] を提供する。利用を希望する場合は、データ利用申請書にJGAD000235（元データ）とJGAD000689（加工データ）を記載すること。","en":"The alignment results [CRAM], variant call results per sample [gVCF], and variant call results per dataset [aggregated VCF] processed JGAD000235 in a certain workflow were provided. If you plan to use the data, please indicate both original data (JGAD000235) and processed data (JGAD000689) on the application form for data use."},"dataProviders":[{"name":{"ja":"中川 英刀","en":"Hidewaki Nakagawa"},"organization":{"name":{"ja":"理化学研究所 生命医科学研究センター","en":"RIKEN Center for Integrative Medical Sciences"}}}],"researchProjects":[],"grants":[],"relatedPublications":[],"datasets":["JGAD000235","JGAD000689","JGAD000888"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"有田 正規","en":"Masanori Arita"},"affiliation":{"ja":"生命情報・DDBJ センター, 国立遺伝学研究所","en":"Bioinformation and DDBJ Center, National Institute of Genetics"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"DDBJ センターと NBDC による JGA 解析データの提供","en":"Provision of processed JGA data analyzed by DDBJ Center and NBDC"},"periodStart":"2021-10-07","periodEnd":"2030-03-31","datasets":["JGAD000235"]},{"principalInvestigator":{"ja":"河野 隆志","en":"Takashi Kohno"},"affiliation":{"ja":"ゲノム生物学研究分野, 国立研究開発法人国立がん研究センター研究所","en":"Division of Genome Biology, National Cancer Center Research Institute"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"AYA(Adolescence and Young Adult)世代がんの個別化予防に資する遺伝要因の同定を目指す研究","en":"Identification of genetic risk factors in AYA(Adolescence and Young Adult) cancer"},"periodStart":"2021-12-02","periodEnd":"2028-12-31","datasets":["JGAD000235"]}]}