{"id":"hum0156","version":1,"url":"https://humandbs.dbcls.jp/research/hum0156/v1","datePublished":"2021-07-30","versions":[{"version":1,"datePublished":"2021-07-30"}],"title":{"ja":"次世代シークエンサーを用いた遺伝子統合解析による食道癌化学放射線療法耐性の解明","en":"Integrative genetic analysis using next generation sequencer reveals mechanisms of resistance to chemoradiotherapy for esophageal cancer"},"summary":{"aims":{"ja":"食道がんに対する化学放射線療法の耐性機構を明らかにする","en":"To clarify mechanisms of resistance to chemoradiotherapy for esophageal cancer"},"methods":{"ja":"内視鏡生検もしくは切除検体から採取した腫瘍組織と末梢血（正常組織）から抽出したDNAを用いた全エクソンシーケンス解析","en":"DNA was extracted from peripheral blood cells and tumor tissues obtained by endoscopyc biopsy or surgery. Then whole exome sequencing was performed."},"targets":{"ja":"化学放射線治療を受けた食道がん33症例","en":"Esophageal cancers treated with chemoradioherapy"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"食道癌：33症例\n（日本人）","en":"33 esophageal cancer patients\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome）","en":"NGS\n(Exome)"}},"releaseNote":{"ja":"食道がん33症例の腫瘍組織および末梢血から抽出したDNAを用いたExome解析の結果をfastqファイルにて提供する。Agilent SureSelect XT Human All Exon V5＋RegによりExon領域にしぼり、Illumina HiSeq 2500にて平均長100塩基を解読した（Paired-end）。","en":"DNAs extracted from tumor tissues and peripheral blood cells from 33 esophageal cancer patients were used for the whole exome sequencing analysis (fastq files). Exons were narrowed down by using of Agilent SureSelect XT Human All Exon V5 + Reg and read by Illumina HiSeq 2500 (Paired-end: 100 bp)."},"dataProviders":[{"name":{"ja":"三森 功士","en":"Koshi Mimori"},"organization":{"name":{"ja":"九州大学病院 別府病院 外科","en":"Department of Surgery, Kyushu University Beppu Hospital"}}}],"researchProjects":[],"grants":[{"title":{"ja":"化学放射線耐性を生む食道癌ゲノム・免疫応答のクローン進化の解明","en":"Clonal evolution of esophageal cancer genome and immune response that produce chemoradiotherapy resistance"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["19K08123"]},{"title":{"ja":"食道癌ゲノム・エピゲノムの腫瘍内不均一性解析による化学放射線療法耐性機構の解明","en":"Analysis of genomic and epigenomic intratumoral heterogeneity reveals mechanisms of resistance to chemoradiotherapy in esophageal cancer"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["16K10397"]},{"title":{"ja":"次世代シークエンサーを用いた遺伝子統合解析による食道癌化学放射線療法耐性の解明","en":"Next-generation sequencing approach detect chemoradiotherapy response in esophageal cancer"},"agency":{"ja":"科学研究費助成事業 若手研究（B）","en":"KAKENHI Grant-in-Aid for Young Scientists (B)"},"grantIds":["26861003"]}],"relatedPublications":[{"title":"The Evolving Genomic Landscape of Esophageal Squamous Cell Carcinoma Under Chemoradiotherapy","doi":"https://doi.org/10.1158/0008-5472.CAN-21-0653","datasets":["JGAD000319"]}],"datasets":["JGAD000319"],"controlledAccessUsers":[]}