{"id":"hum0145","version":1,"url":"https://humandbs.dbcls.jp/research/hum0145/v1","datePublished":"2020-06-19","versions":[{"version":1,"datePublished":"2020-06-19"}],"title":{"ja":"消化器癌患者の末梢血液中における転移再発の予測因子候補に関する臨床的意義の確立","en":"The genetic analysis of circulating tumor DNA in blood of the digestive cancer patients to investigate the prognostic factors of metastasis and reccurence."},"summary":{"aims":{"ja":"大腸がんの転移再発を正確に予測するバイオマーカーの同定","en":"To identify the biomarkers of predicting metastasis and reccurence in colorectal cancer (CRC)."},"methods":{"ja":"以下の組織より抽出したDNAを対象としたTarget capture deep sequencing\n（1）術前化学療法前に内視鏡により採取した原発巣組織\n（2）術前化学療法後、および、転移再発し切除した場合は凍結した外科的切除標本より採取した腫瘍組織と近隣の正常腸管粘膜組織（非腫瘍組織）\n（3）手術時採取した血液","en":"Genomic DNAs were extracted from several samples (as shown below) and performed a target capture deep sequencing analysis by using of the HaloPlex Cancer Research Panel.\n1) Tumor tissues obtained from 3-5 locations in the primary tumor by endoscopic biopsy prior to neoadjuvant chemotherapy (NAC)\n2) Tumor tissues and adjacent normal intestinal mucosa (non-tumor tissues) obtained from fresh-frozen surgically resected specimens after NAC and resecting metastais lesions, if patients were experienced recurrence\n3) Blood samples obtained from patients at the time of surgery"},"targets":{"ja":"術前化学療法後に外科的切除を行なった進行大腸癌10症例","en":"Ten colorectal cancer patients with locally advanced primary tumors who underwent primary tumor resection following NAC"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"大腸癌：10症例\n（日本人）","en":"10 colorectal cancers\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Target Capture）","en":"NGS\n(Target Capture)"}},"releaseNote":{"ja":"術前化学療法後に外科的切除を行なった進行大腸癌10症例の原発巣、手術切除切片の腫瘍組織と非腫瘍組織、末梢血血漿、転移巣切除切片の腫瘍組織から抽出したDNAを用いたHaloPlex Cancer Research PanelによるTarget Capture Sequencing解析データをfastqファイルにて提供する。","en":"DNAs extracted from tumor tissues (primary tumor obtained prior to NAC / after NAC, and metastatic tumor), normal intestinal mucosa, and blood plasma from 10 colorectal cancer patients were used for a target capture deep sequencing by using of the HaloPlex Cancer Research Panel. Fastq files are provided. ."},"dataProviders":[{"name":{"ja":"三森 功士","en":"Koshi Mimori"},"organization":{"name":{"ja":"九州大学病院 別府病院 外科","en":"Department of Surgery, Kyushu University Beppu Hospital"}}}],"researchProjects":[],"grants":[{"title":{"ja":"網羅的遺伝子異常解析と数理学的シミュレーションにもとづく大腸癌肝転移に対する肝切除前化学療法のprecision medicine構築","en":"Construction of precision medicine about neoadjuvant chemotherapy for hepatic metastasis of colorectal cancer by using comprehensive gene aberration analysis and mathematical simulation."},"agency":{"ja":"公益財団法人新日本先進医療研究財団 平成29年度助成金（2017）","en":"The Shinnihon Foundation of Advanced Medical Treatment Research (2017)"},"grantIds":null}],"relatedPublications":[{"title":"Serial mutational tracking in surgically resected locally advanced colorectal cancer with neoadjuvant chemotherapy.","doi":"https://doi.org/10.1038/s41416-018-0208-5","datasets":["JGAD000313"]}],"datasets":["JGAD000313"],"controlledAccessUsers":[]}