{"id":"hum0140","version":1,"url":"https://humandbs.dbcls.jp/research/hum0140/v1","datePublished":"2019-11-19","versions":[{"version":1,"datePublished":"2019-11-19"}],"title":{"ja":"microRNA/mRNA網羅的発現解析及び全エクソンシークエンス解析に基づく直腸癌術前治療効果予測法の開発","en":"Prediction of response to preoperative chemoradiotherapy in rectal cancer based on whole-exome sequencing and transcriptomic analysis"},"summary":{"aims":{"ja":"術前に化学放射線療法を受けた直腸がん症例における分子プロファイリングをもとにした治療効果予測法の確立","en":"Establishment of novel prediction method of preoperative chemoradiothearpeutic effect based on the molecular profiling in rectal cancer patients"},"methods":{"ja":"Illumina HiSeq 2000を使用したExome解析","en":"Exome analysis by using Illumina Hiseq 2000"},"targets":{"ja":"術前に化学放射線療法を受けた直腸がん74症例（原発巣腫瘍組織および非腫瘍組織）","en":"74 rectal cancer patients treated with preoperative chemoraidotherapy"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"術前放射線化学療法施行直腸癌：74症例\n（日本人）","en":"74 rectal cancer treated with preoperative chemoraidotherapy\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome）","en":"NGS\n(Exome)"}},"releaseNote":{"ja":"術前に化学放射線療法を受けた直腸がん74症例の原発巣（腫瘍組織）および非腫瘍組織（もしくは末梢血）から抽出したDNAを用いたExome解析の結果をfastqファイルにて提供する。SureSelect Custom(v5n)及びSureSelect Custom(v5)を用いてExon領域にしぼりIllumina [HiSeq 2000]にて平均長200塩基のDNA断片を解読した（Paired-end）。","en":"DNAs extracted from non-tumor tissues (or blood cells as normal tissues) and the primary tumor tissues from 74 rectal cancer treated with preoperative chemoraidotherapy were used for whole exome sequencing analysis (fastq files). Exons were narrowed down by using of SureSelect Custom(v5n) or SureSelect Custom(v5) and read by Illumina [HiSeq 2000] (paired-end: 200 bp)."},"dataProviders":[{"name":{"ja":"秋吉 高志","en":"Takashi Akiyoshi"},"organization":{"name":{"ja":"がん研有明病院","en":"The Cancer Institute Hospital of JFCR"}}}],"researchProjects":[],"grants":[{"title":{"ja":"全エクソンシークエンスによる直腸癌術前放射線化学療法効果予測遺伝子の同定","en":"Genomic Profile of Responders to Preoperative Chemoradiotherapy for Advanced Rectal Cancer"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["15K10156"]}],"relatedPublications":[{"title":"Immunogenomic profiles associated with response to neoadjuvant chemoradiotherapy in patients with rectal cancer.","doi":"https://doi.org/10.1002/bjs.11179","datasets":["JGAD000236"]}],"datasets":["JGAD000236"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"白石 航也","en":"Kouya Shiraishi"},"affiliation":{"ja":"ゲノム生物学研究分野, 国立研究開発法人国立がん研究センター研究所","en":"Division of Genome Biology, National Cancer Research Institute"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"ゲノム解析に基づく宿主並びに腫瘍における免疫応答ネットワーク機構の解明","en":"Elucidation of immune-system networks between host and tumor based on genomic analysis"},"periodStart":"2019-08-05","periodEnd":"2029-03-31","datasets":["JGAD000236"]}]}