{"id":"hum0129","version":1,"url":"https://humandbs.dbcls.jp/research/hum0129/v1","datePublished":"2018-04-06","versions":[{"version":1,"datePublished":"2018-04-06"}],"title":{"ja":"神経疾患患者からのiPS細胞の樹立とそれを用いた疾患解析に関する研究","en":"Research for analyzing mechanistic insight into disease progression of neural disease based on iPS cell technology"},"summary":{"aims":{"ja":"CHARGE症候群患者における中枢神経系の機能解析","en":"Functional analysis of neural cells derived from CHARGE syndrome patients' fibroblast cells"},"methods":{"ja":"CHARGE症候群患者由来線維芽細胞から樹立したiPS細胞溶解液、および、iPS細胞を神経上皮様細胞（神経前駆細胞）へ分化誘導した細胞溶解液に対して免疫沈降（抗ヒストン抗体：H3K27ac [Acetyl Lys27]）を実施後、DNAを調整してChIP-seqを実施した。","en":"Establishment of iPS cells from CHARGE syndrome patients' fibroblast cells"},"targets":{"ja":"CHARGE症候群2症例（CH1、CH2）","en":"iPS cells or neuroepithelial-like stem cells induced from iPS cells established from CHARGE syndrome patients' skin fibroblast cells"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"ヒストン修飾","en":"Histone modification"},"targets":{"ja":"CHARGE症候群：2症例\n（日本人）","en":"2 CHARGE syndrome patients\n(Japanese)"},"typeOfData":{"ja":"NGS\n（ChIP-seq）","en":"NGS\n(ChIP-seq)"}},"releaseNote":{"ja":"CHARGE症候群患者由来皮膚線維芽細胞より樹立したiPS細胞から誘導した神経上皮様細胞より抽出したDNAを用いたChIP-seq解析の結果をfastqファイルにて提供する。抗ヒストン抗体（H3K27ac）を用いて免疫沈降後、KAPA Hyper Preparation Kitを用いてChIP-seqライブラリを作製し、Illumina HiSeq 2500によりシングルエンドシークエンシングを行っている（50 bp）。","en":"DNAs extracted from long-term self-renewing neuroepithelial-like stem cells induced from iPS cells established of skin fibroblasts from CHARGE syndrome patients were used for ChIP-seq analyses (fastq format files). Anti-histone antibody (H3K27ac) was used for immunoprecipitation. ChIP-Seq libraries were prepared by using KAPA Hyper Preparation Kit and sequenced by Illumina HiSeq 2500 (50 bp, single-end)."},"dataProviders":[{"name":{"ja":"岡野 栄之","en":"Hideyuki Okano"},"organization":{"name":{"ja":"慶應義塾大学医学部","en":"Keio University School of Medicine"}}}],"researchProjects":[],"grants":[{"title":{"ja":"iPS 細胞由来神経前駆細胞を用いた脊髄損傷・脳梗塞の再生医療","en":"Regenerative medicine for spinal cord injury and stroke using human induced pluripotent stem cell-derived neural precursor cells"},"agency":{"ja":"日本医療研究開発機構（AMED） 再生医療実現拠点ネットワークプログラム 疾患・組織別実用化研究拠点","en":"Research Center Network for Realization of Regenerative Medicine Centers for Clinical Application Research on Specific Disease/Organ, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP16bm0204001"]},{"title":{"ja":"ヒト iPS 由来神経前駆細胞の腫瘍形成能のメカニズムとその制御による安全性 確保の検討","en":"Mechanism of tumorigenesis of human iPSC-derived neural progenitor/stem cells"},"agency":{"ja":"日本医療研究開発機構（AMED） 再生医療実用化研究事業","en":"Research Project for Practical Application of Regenerative Medicine, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP15bk0104009"]}],"relatedPublications":[{"title":"Chromatin remodeler CHD7 regulates the stem cell identity of human neural progenitors","doi":"https://doi.org/10.1101/gad.301887.117","datasets":["JGAD000142"]}],"datasets":["JGAD000142"],"controlledAccessUsers":[]}