{"id":"hum0127","version":4,"url":"https://humandbs.dbcls.jp/research/hum0127/v4","datePublished":"2024-07-10","versions":[{"version":1,"datePublished":"2020-04-14"},{"version":2,"datePublished":"2021-03-08"},{"version":3,"datePublished":"2024-01-22"},{"version":4,"datePublished":"2024-07-10"}],"title":{"ja":"マルチオーミクス解析を用いたがん患者評価に基づく個別化治療と新技術開発に関する研究ープロジェクトHOPE（High-tech Omics-based Patients Evaluation）for Cancer Therapyー","en":"Implementation of individualized medicine for cancer patients by multiomics-based analyses - the Project HOPE (High-tech Omics-based Patients Evaluation) for Cancer Therapy"},"summary":{"aims":{"ja":"静岡がんセンター手術症例のうち、年間約1,000症例のがん患者から提供された血液や手術摘出組織について、ゲノム、トランスクリプトーム、プロテオーム、メタボローム、メチロームなど最先端マルチオミクス解析技術を用いてがんの発生や進展過程に関与する分子生物学的特徴を分析する。 腫瘍組織については、一人ひとりのがん患者について、がんのドライバー遺伝子や遺伝性がん原因遺伝子を突き止める。 さらに、血液細胞を用いた生殖細胞系列のゲノム解析により、遺伝性がんの存在について検討する。薬物代謝酵素の遺伝子多型を調べ、薬剤感受性や副作用のについての関連を検討する。 得られた成果は、新たな予後因子、創薬、診断薬開発など、新規がん診療技術の開発に役立てられる。一人ひとりの患者についての解析結果が、治療の実践においてどのように役立つかについても検討する。","en":"Blood and surgically removed tissues donated by approximately 1,000 cancer patients annually from Shizuoka Cancer Center's surgical cases are analyzed using multi-omics analysis technologies including genome, transcriptome, proteome, metabolome, and methylome. Tumor tissue analyses identified cancer driver genes and inherited cancer-causing genes in individual cancer patients and clarified the molecular profiling and sequential somatic mutation shift in hypermutator tumors harborig POLE mutations. Germline genome analysis will also be used to examine the presence or absence of hereditary cancers. Genetic polymorphisms of drug-metabolizing enzymes will be examined for associations with drug sensitivity and side effects. These results will be used to develop new drug discovery, diagnostics, and other treatment technologies."},"methods":{"ja":"末梢血の白血球（非腫瘍組織）や手術摘出組織（腫瘍組織、非腫瘍組織）から抽出したDNAおよびRNAを対象に、NGSによるWhole Exome seq、DNA amplicon-seq、RNA amplicon-seq、マイクロアレイ、Whole genome sequencing、メチル化解析を実施する。","en":"DNAs and RNAs extracted from peripheral blood cells, tumor and non-tumor tissues are analyzed by whole-exome sequencing, DNA amplicon sequencing, RNA amplicon sequencing, microarray, Whole genome sequencing, and Methylation array."},"targets":{"ja":"JGAS000130：いずれかのがんを発症し、静岡がんセンター病院において摘出手術を受けた2042症例のうち、POLE遺伝子内に変異を持ち、かつ、がん関連変異を高頻度で保有する91症例\nJGAS000274：いずれかのがんを発症し、静岡がんセンター病院において摘出手術を受けた5143症例\nJGAS000604：消化管間質腫瘍：30症例（23症例は上記で登録済み、7症例は新規）\nJGAS000715：静岡がんセンター病院で手術を受けたがん患者5143症例において全エクソンシーケンシングおよび全遺伝子発現解析などでドライバー遺伝子に変化が認められなかった177症例","en":"JGAS000130: 91 hypermutated solid tumors obtained from patients who had surgeries to remove cancers at the Shizuoka Cancer Center Hospital\nJGAS000274: Fresh frozen tumors and blood cells obtained from 5143 cancer patients who had surgeries to remove cancers at the Shizuoka Cancer Center Hospital\nJGAS000604: Gastrointestinal stromal tumor (GIST) 30 cases\nJGAS000715: 177 cases in which no changes in driver genes were detected by whole exon sequencing and whole gene expression analysis in 5143 cancer patients who underwent surgery at Shizuoka Cancer Centre Hospital."},"url":{"ja":[{"url":"https://www.scchr.jp/project-hope.html","text":"https://www.scchr.jp/project-hope.html"}],"en":[{"url":"https://www.scchr.jp/en/institute/projecthope.html","text":"https://www.scchr.jp/en/institute/projecthope.html"}]}},"listingSummary":{"methods":{"ja":"配列決定\n発現","en":"Sequencing, Expression profiling"},"targets":{"ja":"POLE変異および高変異症例の癌：91症例\n癌：5143＋7症例\n（日本人）","en":"91 cancer patients with hypermutator tumors harborig POLE mutations\n5143 + 7 cancer patients\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome、DNA Amplicon-seq、RNA Amplicon-seq、WGS）\nマイクロアレイ\nメチル化アレイ","en":"NGS\n(Exome, DNA Amplicon-seq, RNA Amplicon-seq, WGS)\nMicroarray\nMethylation array"}},"releaseNote":{"ja":"静岡がんセンター病院にて手術を受けたがん患者5143症例の内、全エクソンシーケンシングおよび全遺伝子発現解析などでドライバー遺伝子に変化が認められなかった177症例の腫瘍組織と末梢血白血球から抽出したDNAを用いたWGS解析を実施。腫瘍組織と非腫瘍組織の塩基配列を比較し、腫瘍特異的な変異情報を提供する（tab、vcf）。","en":"DNAs extracted from peripheral blood cells (non-tumor cells) and surgically dissected tumor and non-tumor tissues from 177 cancer patients in which no changes in driver genes were detected by whole exon sequencing and whole gene expression analysis were used for whole genome sequencing analysis. Variant information was provided only in the tumor tissues (tab, vcf). Whole Genome Sequencing was performed using NovaSeq 6000 (Illumina)."},"dataProviders":[{"name":{"ja":"山口 建","en":"Ken Yamaguchi"},"organization":{"name":{"ja":"静岡県立静岡がんセンター","en":"Shizuoka Cancer Center"}}}],"researchProjects":[{"name":{"ja":"プロジェクトHOPE (High-tech Omics-based Patient Evaluation)（がんのマルチオミクス解析）/ 静岡がんセンター","en":"Project HOPE (High-tech Omics-based Patient Evaluation) / Shizuoka Cancer Center"},"url":{"ja":[{"url":"https://www.scchr.jp/project-hope.html","text":"https://www.scchr.jp/project-hope.html"}],"en":[{"url":"https://www.scchr.jp/en/institute/projecthope.html","text":"https://www.scchr.jp/en/institute/projecthope.html"}]}}],"grants":[],"relatedPublications":[{"title":"Implementation of individualized medicine for cancer patients by multiomics-based analyses-the Project HOPE-","doi":"https://doi.org/10.2220/biomedres.35.407","datasets":null},{"title":"Molecular profiling and sequential somatic mutation shift in hypermutator tumours harbouring POLE mutations","doi":"https://doi.org/10.1038/s41598-018-26967-4","datasets":["JGAD000141"]},{"title":"Integrated next-generation sequencing analysis of whole exome and 409 cancer-related genes","doi":"https://doi.org/10.2220/biomedres.37.367","datasets":null},{"title":"Integrated analysis of gene expression and copy number identified potential cancer driver genes with amplification-dependent overexpression in 1,454 solid tumors","doi":"https://doi.org/10.1038/s41598-017-00219-3","datasets":null},{"title":"Next generation sequencing approach for detecting 491 fusion genes from human cancer","doi":"https://doi.org/10.2220/biomedres.37.51","datasets":null},{"title":"Japanese version of The Cancer Genome Atlas, JCGA, established using fresh frozen tumors obtained from 5143 cancer patients","doi":"https://doi.org/10.1111/cas.14290","datasets":["JGAD000380"]},{"title":"Whole-genome and epigenomic landscapes of malignant gastrointestinal stromal tumors harboring KIT exon 11 557-558 deletion mutations","doi":"https://doi.org/10.1158/2767-9764.crc-22-0364","datasets":["JGAD000733"]}],"datasets":["JGAD000141","JGAD000380","JGAD000733","JGAD000848"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"万代 昌紀","en":"Masaki Mandai"},"affiliation":{"ja":"婦人科学産科学教室, 京都大学医学部医学研究科","en":"department of Gynecology and Obstetrics, Kyoto University Faculty of Medicene"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"多様な臨床情報を考慮した婦人科悪性腫瘍患者のオミックス解析（全ゲノム・全トランスクリプトーム・プロテオーム・メタボローム解析）による個別化治療の探索","en":"Integrated analyses of omics (genomics, transcriptomics, proteomics and metabolomics) associated with clinical variables for developing indivisualizedtreatment in gynecological malignancy"},"periodStart":"2018-10-04","periodEnd":"2025-04-14","datasets":["JGAD000141","JGAD000380"]},{"principalInvestigator":{"ja":"三森 功士","en":"Koshi Mimori"},"affiliation":{"ja":"外科, 九州大学病院別府病院","en":"Department of Surgery, Kyushu University Beppu Hospital"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"トランスオミクス解析による日本人乳癌の分子的特徴の解明","en":"Transomic analysis of molecular characterization of Japanese breast cancer"},"periodStart":"2021-06-01","periodEnd":"2025-08-05","datasets":["JGAD000141","JGAD000380"]},{"principalInvestigator":{"ja":"松村 謙臣","en":"Noriomi Matsumura"},"affiliation":{"ja":"産科婦人科学教室, 近畿大学医学部","en":"Obstetrics and Gynecology, Kindai University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"JGOG3017-TR1：エクソームシークエンスデータを有する卵巣明細胞癌のRNAseq解析","en":"Integrated multi-omics analysis for ovarian clear cell adenocarcinoma: JGOG3017-TR1"},"periodStart":"2024-10-15","periodEnd":"2027-12-31","datasets":["JGAD000141","JGAD000380","JGAD000733","JGAD000848"]},{"principalInvestigator":{"ja":"山口 建","en":"Ken Yamaguchi"},"affiliation":{"ja":"婦人科学産婦人科学教室, 京都大学","en":"Gynecology and Obstetrics, Kyoto University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"JGOG3017-TR1：エクソームシークエンスデータを有する卵巣明細胞癌のRNAseq解析","en":"Integrated multi-omics analysis for ovarian clear cell adenocarcinoma: JGOG3017-TR1"},"periodStart":"2024-10-22","periodEnd":"2027-12-31","datasets":["JGAD000848"]}]}