{"id":"hum0125","version":1,"url":"https://humandbs.dbcls.jp/research/hum0125/v1","datePublished":"2018-11-05","versions":[{"version":1,"datePublished":"2018-11-05"}],"title":{"ja":"制御性T細胞特異的エピゲノム誘導による免疫制御の研究","en":"Immune regulation by cotrolling regulatory T cell-specific epigenome"},"summary":{"aims":{"ja":"自己免疫疾患における免疫担当細胞の解析","en":"Analysis of immune cells in autoimmune diseases"},"methods":{"ja":"RNA-seq","en":"RNA-seq"},"targets":{"ja":"クローン病3症例の病変部および大腸がん4症例の切除切片の中の非腫瘍組織（正常大腸組織）について、分離マーカーの違い（CD14-CD11c- / CD14-CD11c- / CD14+CD11c-CD163low）によって単離した細胞から抽出したRNA","en":"Lesions from Crohn's disease patients and normal colorectal tissues from colorectal cancer patients"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"クローン病：3症例\n大腸癌：4症例（非腫瘍組織）\n（日本人）","en":"3 Crohn's diseases\n4 Colorectal cancers (normal cells)\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"クローン病3症例の病変部および大腸がん4症例の切除切片の中の非腫瘍組織（正常大腸組織）について、分離マーカーの違い（CD14-CD11c- / CD14-CD11c- / CD14+CD11c-CD163low）によって単離した細胞から抽出したRNAを用いたRNA-Seq解析データをfastqファイルにて提供する。KAPA Library Preparation KitによるRNAライブラリ作製後、Thermo Fisher Scientific [Ion S5]にて平均長150 bpを読んだ（Single-end）。","en":"RNAs were extracted from isolated cells using different markers (CD14-CD11c- / CD14-CD11c- / CD14+CD11c-CD163low) from tumor tissues of Crohn's diseases and non-tumor tissues of colorectal cancers. Libraries for RNA-seq were prepared with KAPA Library Preparation Kit and RNA-seq was performed with Thermo Fisher Scientific [Ion S5] (150 bp on average, Single-end). RNA-seq data are provided as fastq."},"dataProviders":[{"name":{"ja":"坂口 志文","en":"Shimon Sakaguchi"},"organization":{"name":{"ja":"大阪大学免疫学フロンティア研究センター 実験免疫学","en":"Experimental Immunology, IFReC, Osaka University"}}}],"researchProjects":[],"grants":[{"title":{"ja":"炎症性腸疾患の病態解析","en":"Analysis of the pathology of inflammatory bowel disease"},"agency":{"ja":"科学研究費助成事業 基盤研究（A）","en":"KAKENHI Grant-in-Aid for Scientific Research (A)"},"grantIds":["15H02511"]},{"title":{"ja":"炎症性腸疾患における抗原提示細胞の異常活性化の機序および機能解析","en":"Mechanism and function analysis of abnormal activation of antigen presenting cells in inflammatory bowel disease"},"agency":{"ja":"科学研究費助成事業 若手研究（B）","en":"KAKENHI Grant-in-Aid for Young Scientists (B)"},"grantIds":["17K16546"]}],"relatedPublications":[{"title":"Innate Myeloid Cell Subset-Specific Gene Expression Patterns in the Human Colon are Altered in Crohn's Disease Patients","doi":"https://doi.org/10.1159/000490890","datasets":["JGAD000138"]}],"datasets":["JGAD000138"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000138"]}]}