{"id":"hum0122","version":1,"url":"https://humandbs.dbcls.jp/research/hum0122/v1","datePublished":"2020-01-14","versions":[{"version":1,"datePublished":"2020-01-14"}],"title":{"ja":"市販ヒト初代細胞由来iPS 細胞のゲノム変異解析","en":"Mutation analysis of iPS cells derived from commercially-avaialble human primary cells"},"summary":{"aims":{"ja":"ゲノム初期化におけるゲノム不安定性を明らかにする","en":"Elucidation of genome instability during genome reprogramming"},"methods":{"ja":"樹立したiPS細胞、および樹立に用いた初代細胞について、全ゲノムシークエンシングおよびSNPアレイを実施し、変異解析を行う。","en":"Whole genome sequencing and copy number variation analysis using SNP array of iPS cells and their parental cells to identify genetic abberations"},"targets":{"ja":"市販のヒト培養細胞、およびそれから誘導したiPS細胞株","en":"Human iPS cells derived from commercially-available primary cells"},"url":{"ja":[{"url":"https://www.qst.go.jp/","text":"https://www.qst.go.jp/"}],"en":[{"url":"https://www.qst.go.jp/site/qst-english/","text":"https://www.qst.go.jp/site/qst-english/"}]}},"listingSummary":{"methods":{"ja":"配列決定\nゲノムワイド","en":"Sequencing, Genome-wide"},"targets":{"ja":"市販のヒト培養細胞、およびそれから誘導したiPS細胞株：計27検体\n（細胞株）","en":"human iPS cells and their parental cells: total 27 samples\n(Cell-line)"},"typeOfData":{"ja":"NGS\n（WGS）\nSNP-chip","en":"NGS\n(WGS)\nSNP-chip"}},"releaseNote":{"ja":"市販のヒト培養細胞（成人皮膚線維芽細胞、ヒト臍帯血由来単核細胞）と、培養細胞から誘導したiPS細胞から抽出したDNAを用いた、全ゲノムシークエンシングおよびSNPアレイ解析結果をfastqおよびidat/txtファイル形式にて提供する。","en":"Whole genome sequencing and SNP array were performed using DNAs extracted from human iPS cells and their parental cells. The data are provided as fastq and idat/txt format files."},"dataProviders":[{"name":{"ja":"荒木 良子","en":"Ryoko Araki"},"organization":{"name":{"ja":"研究開発法人量子科学技術研究開発機構 放射線医学総合研究所 放射線障害治療研究部","en":"National Institutes for Quantum and Radiological Science and Technology, National Institute of Radiological Sciences, Department of Basic Medical Sciences for Radiation Damages"}}},{"name":{"ja":"安倍 真澄","en":"Masumi Abe"},"organization":{"name":{"ja":"研究開発法人量子科学技術研究開発機構 放射線医学総合研究所 放射線障害治療研究部","en":"National Institutes for Quantum and Radiological Science and Technology, National Institute of Radiological Sciences, Department of Basic Medical Sciences for Radiation Damages"}}}],"researchProjects":[{"name":{"ja":"幹細胞研究グループ","en":"Stem cell biology group"},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"ゲノム初期化に伴う点突然変異と活性酸素","en":"Point mutations associated with genome reprogramming"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["17H03615"]},{"title":{"ja":"ゲノムワイド放射線影響解析法の開発","en":"Development of genome-wide analysis for radiation effects"},"agency":{"ja":"科学研究費助成事業 挑戦的萌芽研究","en":"KAKENHI Grant-in-Aid for challenging Exploratory Research"},"grantIds":["17K20053"]}],"relatedPublications":[{"title":"Genetic aberrations in iPSCs are introduced by a transient G1/S cell cycle checkpoint deficiency","doi":"https://doi.org/10.1038/s41467-019-13830-x","datasets":["DRA006622","DRA007336","DRA008459","E-GEAD-311"]}],"datasets":["DRA006622","DRA007336","DRA008459","E-GEAD-311"],"controlledAccessUsers":[]}