{"id":"hum0120","version":3,"url":"https://humandbs.dbcls.jp/research/hum0120/v3","datePublished":"2021-12-06","versions":[{"version":1,"datePublished":"2020-04-03"},{"version":2,"datePublished":"2020-10-30"},{"version":3,"datePublished":"2021-12-06"},{"version":4,"datePublished":"2022-07-29"}],"title":{"ja":"消化器癌患者の末梢血液中における転移再発の予測因子候補に関する臨床的意義の確立","en":"The genetic analysis of circulating tumor DNA in blood of the digestive cancer patients to investigate the prognostic factors of metastasis and reccurence."},"summary":{"aims":{"ja":"大腸がん、胃がん、乳がんの転移再発を正確に予測するバイオマーカーの同定","en":"To identify the biomarkers of predicting metastasis and recurrence in colorectal cancer (CRC), gastric cancer, and breast cancer."},"methods":{"ja":"原発巣・転移巣の切除切片（腫瘍組織および正常粘膜）から抽出したDNA/RNAを用いた全エクソームシーケンス、RNA-seq、およびT細胞のレパトア解析、ならびにメチレーションアレイおよびWhole genome bisulfite sequencing解析。治療前後で経時的に血漿サンプルを採取し、抽出した血中循環腫瘍DNAを用いたTarget Capture Sequencing解析。","en":"- DNAs and RNAs were extracted from the tumor and matched normal tissues. Whole exome sequencing was performed using tumor and matched normal tissues. RNA-seq, TCR repertoire, methylation array, and whole genome bisulfite sequencing analyses were performed using tumor tissues.\n- Circulating tumor DNA were extracted from patients plasma and targeted sequence were performed."},"targets":{"ja":"原発巣切除後に肺・肝転移をきたしたstage 1、2の大腸がん、胃がん、乳がん","en":"10 cases of stage 1 and 2 CRCs, which cases occurred liver or lung metastasis."},"url":{"ja":null,"en":null}},"listingSummary":{},"releaseNote":{"ja":"大腸がん、胃がん、乳がんそれぞれ16症例の原発巣の腫瘍組織から抽出したDNAを用いたメチル化アレイ解析、並びに、大腸がん9症例の原発巣および転移巣の腫瘍組織から抽出したDNAを用いたWGBS解析データをidatおよびfastqファイルにて提供する。","en":"DNAs extracted from tumor tissues (the primary tumor) from 16 colorectal cancer, 16 gastric cancer, and 16 breast cancer patients and DNAs extracted from tumor tissues (primary and metastatic tumor) from 9 colorectal cancer patients were used for the methylation array and WGBS analysis respectively. IDAT and Fastq files are provided."},"dataProviders":[{"name":{"ja":"三森 功士","en":"Koshi Mimori"},"organization":{"name":{"ja":"九州大学病院 別府病院 外科","en":"Department of Surgery, Kyushu University Beppu Hospital"}}}],"researchProjects":[],"grants":[{"title":{"ja":"微小環境多様性に連動する難治がんの分子遺伝学的多様性創成機構の解明と新たながん治療法・予測医療技術の開発","en":"Understanding of molecular mechanisms to generate genetic heterogeneity associated with micro-environmental diversity for development of novel therapy and diagnostic technology in intractable cancers."},"agency":{"ja":"日本医療研究開発機構（AMED） 次世代がん医療創生研究事業（P-CREATE）","en":"Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP19cm0106504"]}],"relatedPublications":[{"title":"Pan-cancer methylome analysis for cancer diagnosis and classification of cancer cell of origin.","doi":"https://doi.org/10.1038/s41417-021-00401-w","datasets":["E-GEAD-396","DRA010902"]}],"datasets":["JGAD000311","DRA011183","DRA011184","DRA011185","E-GEAD-396","DRA010902"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"白石 航也","en":"Kouya Shiraishi"},"affiliation":{"ja":"ゲノム生物学研究分野, 国立研究開発法人国立がん研究センター研究所","en":"Division of Genome Biology, National Cancer Research Institute"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"ゲノム解析に基づく宿主並びに腫瘍における免疫応答ネットワーク機構の解明","en":"Elucidation of immune-system networks between host and tumor based on genomic analysis"},"periodStart":"2019-08-05","periodEnd":"2029-03-31","datasets":["JGAD000311","JGAD000312"]}]}