{"id":"hum0118","version":1,"url":"https://humandbs.dbcls.jp/research/hum0118/v1","datePublished":"2017-12-11","versions":[{"version":1,"datePublished":"2017-12-11"}],"title":{"ja":"バイオマーカー探索に資する網羅的遺伝子解析研究（子宮体がん）","en":"Genome-wide analysis for biomarker exploration (endometrial cancer)"},"summary":{"aims":{"ja":"子宮体がんのリンパ節転移有無を鑑別するバイオマーカー探索","en":"exploration of biomarkers discriminating lymph node metastasis in endometrial cancers"},"methods":{"ja":"CAGE（Cap Analysis of Gene Expression）、サンガー法","en":"CAGE (Cap Analysis of Gene Expression), Sanger sequencing"},"targets":{"ja":"子宮体がん17症例の摘出組織（腫瘍組織）から抽出したRNA","en":"surgically dissected tumor tissues from 17 endometrial cancer patients"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"転写開始点同定\n配列決定","en":"Transcription start site identification, Sequencing"},"targets":{"ja":"子宮体癌：17症例\n（日本人）","en":"17 endometrial cancer patients\n(Japanese)"},"typeOfData":{"ja":"NGS\n（CAGE-seq）\nSanger-seq","en":"NGS (CAGE-seq)\nSanger-seq"}},"releaseNote":{"ja":"子宮体がん17症例から摘出した腫瘍組織からRNAを抽出し、全染色体を対象としたCAGE-seq（Illumina HiSeq 2500）、および、TACC2領域（cDNA）を対象としたサンガー法（Thermo Fisher Scientific (ABI) 3500 Genetic Analyzer）による配列決定を実施した結果をfastqファイルにて提供する。","en":"RNAs extracted from surgically dissected tumor tissues from 17 patients with endometrial cancer were used for CAGE-seq (Illumina HiSeq 2500) and reverse-transcribed samples (complementary DNA [cDNA]) were used for Sanger-seq for TACC2 (Thermo Fisher Scientific (ABI) 3500 Genetic Analyzer). Fastq formated files are provided."},"dataProviders":[{"name":{"ja":"林崎 良英","en":"Yoshihide Hayashizaki"},"organization":{"name":{"ja":"理化学研究所 予防医療・診断技術開発プログラム","en":"RIKEN Preventive Medicine and Diagnosis Innovation Program"}}}],"researchProjects":[{"name":{"ja":"CAGE法による子宮体がんにおける新規バイオマーカーの網羅的解析（順天堂大学 寺尾泰久）","en":"Genome-wide exploration for biomarkers in endometrial cancer by using CAGE (Yasuhisa Terao, Juntendo University)"},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"CAGE法を用いた子宮体癌におけるリンパ節転移予測マーカーの同定","en":"Exploration of biomarkers to predict lymph node metastasis in endometrial cancer by using CAGE"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["15K10732"]},{"title":{"ja":null,"en":null},"agency":{"ja":"理化学研究所 オミックス基盤研究領域 運営費交付金","en":"Research Grant to RIKEN Omics Science Center from MEXT"},"grantIds":null},{"title":{"ja":null,"en":null},"agency":{"ja":"理化学研究所 予防医療・診断技術開発プログラム 運営費交付金","en":"Research Grant to RIKEN Preventive Medicine and Diagnosis Innovation Program from MEXT"},"grantIds":null}],"relatedPublications":[{"title":"Promoter-level transcriptome in primary lesions of endometrial cancer identified biomarkers associated with lymph node metastasis.","doi":"https://doi.org/10.1038/s41598-017-14418-5","datasets":["JGAD000135"]}],"datasets":["JGAD000135"],"controlledAccessUsers":[]}