{"id":"hum0112","version":1,"url":"https://humandbs.dbcls.jp/research/hum0112/v1","datePublished":"2017-12-14","versions":[{"version":1,"datePublished":"2017-12-14"}],"title":{"ja":"ヒト胎盤幹細胞の樹立と細胞特性に関する基礎的研究","en":"Basic research for the establishment and characterization of human trophoblast stem cells"},"summary":{"aims":{"ja":"受精卵よりヒト胎盤幹細胞株を樹立し、その細胞特性に関する基礎的な研究を行う。","en":"To establish human trophoblast stem cells from blastocyst, and to characterize them."},"methods":{"ja":"PBAT-seq、ChIP-seq、RNA-seq、miRNA-seq","en":"PBAT-seq, ChIP-seq, RNA-seq, and miRNA-seq"},"targets":{"ja":"ヒト胎盤幹細胞2系統","en":"trophoblast stem cell (TS)"},"url":{"ja":[{"url":"https://ihec-epigenomes.org/","text":"https://ihec-epigenomes.org/"}],"en":[{"url":"https://ihec-epigenomes.org/","text":"https://ihec-epigenomes.org/"}]}},"listingSummary":{"methods":{"ja":"発現\nメチル化\nヒストン修飾","en":"Expression profiling, Methylation profiling, Histone modification"},"targets":{"ja":"ヒト胎盤幹細胞：2系統\n（日本人）","en":"2 trophoblast stem cells\n(Japanese)"},"typeOfData":{"ja":"NGS（PBAT-seq、ChIP-seq、RNA-seq、miRNA-seq）","en":"NGS\n(PBAT-seq, ChIP-seq, RNA-seq, miRNA-seq)"}},"releaseNote":{"ja":"NGS（PBAT-seq）\nヒト胎盤幹細胞より抽出したDNAを用いたPBAT-seq解析の結果をfastqファイルにて提供する。Post-Bisulfite Adaptor-Tagging（PBAT）法によりライブラリを作製し、Illumina [HiSeq 1500]によりシングルエンドシークエンシングを行っている（101 bp）。\nNGS（ChIP-seq）\nヒト胎盤幹細胞より抽出したDNAを用いたChIP-seq解析の結果をfastqファイルにて提供する。抗H3K4me3, H3K4me1, H3K9me3, H3K27ac, H3K27me3, H3K36me3抗体を用いて免疫沈降後Ovation Ultralow System V2 kit (NuGEN)を用いてChIP-seqライブラリを作製し、Illumina [HiSeq 2500]によりペアエンドシークエンシングを行っている（126 bp）。\nNGS（RNA-seq）\nヒト胎盤幹細胞より抽出したmRNAを用いたRNA-seq解析の結果をfastqファイルにて提供する。TruSeq Stranded mRNA LT Sample Prep Kit (Illumina)を用いてライブラリを作製し、Illumina [HiSeq 2500]によりペアエンドシークエンシングを行っている（101 bp）。\nNGS（miRNA-seq）\nヒト胎盤幹細胞より抽出したRNAを用いたmiRNA-seq解析の結果をfastqファイルにて提供する。NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs)を用いてライブラリを作製し、Illumina [HiSeq 1500]によりシングルエンドシークエンシングを行っている（64 bp）。","en":"NGS (PBAT-seq)\nDNAs extracted from trophoblast stem cell were used for PBAT-seq analyses (fastq format files). Libraries were prepared by Post-Bisulfite Adaptor-Tagging (PBAT) and sequencing was performed with Illumina [HiSeq 1500] (101 bp, single-end).\nNGS (ChIP-seq)\nDNAs extracted from trophoblast stem cell were used for ChIP-seq analyses (fastq format files). Anti H3K4me3, H3K4me1, H3K9me3, H3K27ac, H3K27me3, H3K36me3 antibodies were used for immunoprecipitation. ChIP-Seq libraries were prepared by using Ovation Ultralow System V2 kit (NuGEN) and sequenced using Illumina [HiSeq 2500] (126 bp, paired-end).\nNGS (RNA-seq)\nmRNAs extracted from trophoblast stem cell were used for RNA-seq analyses (fastq format files). Libraries were prepared by using TruSeq Stranded mRNA LT Sample Prep Kit (Illumina) and sequencing was performed with Illumina [HiSeq 2500] (101 bp, paired-end).\nNGS (small RNA-seq)\nRNAs extracted from trophoblast stem cell were used for miRNA-seq analyses (fastq format files). Libraries were prepared by using NEBNext Small RNA Library Prep Set for Illumina (New England Biolabs) and sequencing was performed with Illumina [HiSeq 1500] (64 bp, single-end)."},"dataProviders":[{"name":{"ja":"有馬 隆博","en":"Takahiro Arima"},"organization":{"name":{"ja":"東北大学大学院医学系研究科 環境遺伝医学総合研究センター 情報遺伝学分野","en":"Department of Informative Genetics, Environment and Genome Research Center,Tohoku University Graduate School of Medicine"}}}],"researchProjects":[{"name":{"ja":"革新的先端研究開発支援事業（AMED-CREST） 国際ヒトエピゲノムコンソーシアム（IHEC）佐々木チーム","en":"Core Research and Evolutional Science and Technology (CREST), International Human Epigenome Consortium (IHEC), Sasaki Team"},"url":{"ja":[{"url":"https://crest-ihec.jp/","text":"https://crest-ihec.jp/"}],"en":[{"url":"https://crest-ihec.jp/english/index.html","text":"https://crest-ihec.jp/english/index.html"}]}}],"grants":[{"title":{"ja":"生殖発生にかかわる細胞のエピゲノム解析基盤研究","en":"Epigenome analysis of cells in the placenta and endometrium forming the fetal-maternal interface"},"agency":{"ja":"日本医療研究開発機構 革新的先端研究開発支援事業（AMED-CREST）","en":"Core Research and Evolutional Science and Technology, Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED-CREST)"},"grantIds":["JP17gm0510011"]}],"relatedPublications":[{"title":"Derivation of Human Trophoblast Stem Cells","doi":"https://doi.org/10.1016/j.stem.2017.11.004","datasets":["JGAD000121","JGAD000073","JGAD000115"]},{"title":"The microRNA cluster C19MC confers differentiation potential into trophoblast lineages upon human pluripotent stem cells","doi":"https://doi.org/10.1038/s41467-022-30775-w","datasets":["JGAD000121","JGAD000115"]}],"datasets":["JGAD000121"],"controlledAccessUsers":[{"principalInvestigator":{"en":"Michael Roberts"},"affiliation":{"en":"Animal Sciences/ Biochemistry, University of Missouri"},"country":{"ja":"アメリカ合衆国 (ミズーリ州)","en":"Missouri, United States"},"researchTitle":{"ja":"A comparative analysis of transcriptome data from placental cell line derived from anonymous placental cells","en":"A comparative analysis of transcriptome data from placental cell line derived from anonymous placental cells"},"periodStart":"2018-03-02","periodEnd":"2023-08-28","datasets":["JGAD000121"]},{"principalInvestigator":{"en":"Zhigang Xue"},"affiliation":{"en":"School of Medicine and Life Science, Tongji University"},"country":{"ja":"中国","en":"China"},"researchTitle":{"ja":"Mechanism of cell programming and embryo-gamete original disease in human embryo development.","en":"Mechanism of cell programming and embryo-gamete original disease in human embryo development."},"periodStart":"2018-12-17","periodEnd":"2021-03-19","datasets":["JGAD000121"]},{"principalInvestigator":{"en":"Todd Macfarlan"},"affiliation":{"en":"National Institute of Child Health and Human Development"},"country":{"ja":"アメリカ合衆国 (メリーランド州)","en":"Maryland, United States"},"researchTitle":{"ja":"Analysis of the public methylome, chromatin and transcription profiles for induced human trophoblast stem cells","en":"Analysis of the public methylome, chromatin and transcription profiles for induced human trophoblast stem cells"},"periodStart":"2018-12-17","periodEnd":"2019-09-04","datasets":["JGAD000121"]},{"principalInvestigator":{"en":"Howard Fine"},"affiliation":{"en":"Weill Cornell Medicine"},"country":{"ja":"アメリカ合衆国 (ニューヨーク州)","en":"New York, United States"},"researchTitle":{"ja":"Recapitulation of the trophoblast program during gliomagenesis.","en":"Recapitulation of the trophoblast program during gliomagenesis."},"periodStart":"2018-12-18","periodEnd":"2022-11-21","datasets":["JGAD000121"]},{"principalInvestigator":{"en":"William Yeung"},"affiliation":{"en":"Department of O&G, The University of Hong Kong"},"country":{"ja":"中国","en":"China"},"researchTitle":{"ja":"Derivation of human expanded potential stem cells (EPSCs) from human pre-implantation embryos","en":"Derivation of human expanded potential stem cells (EPSCs) from human pre-implantation embryos"},"periodStart":"2019-02-12","periodEnd":"2020-09-02","datasets":["JGAD000121"]},{"principalInvestigator":{"en":"Thorold Theunissen"},"affiliation":{"en":"Washington University School of Medicine in St. Louis"},"country":{"ja":"アメリカ合衆国 (ミズーリ州)","en":"Missouri, United States"},"researchTitle":{"ja":"Assessment of trophoblast fates derived from human pluripotent stem cells","en":"Assessment of trophoblast fates derived from human pluripotent stem cells"},"periodStart":"2019-12-10","periodEnd":"2023-07-05","datasets":["JGAD000121"]},{"principalInvestigator":{"en":"Hu Li"},"affiliation":{"en":"Department of Histoembryology, Genetics and Developmental Biology, Shanghai JiaoTong University School of Medicine"},"country":{"ja":"中国","en":"China"},"researchTitle":{"ja":"Derivation of Human induced Trophoblast Stem Cells from Embryonic Stem Cells","en":"Derivation of Human induced Trophoblast Stem Cells from Embryonic Stem Cells"},"periodStart":"2020-01-15","periodEnd":"2024-01-30","datasets":["JGAD000121"]},{"principalInvestigator":{"en":"Jonghwan Kim"},"affiliation":{"en":"Department of Molecular Biosciences, University of Texas at Austin"},"country":{"ja":"アメリカ合衆国 (テキサス州)","en":"Texas, United States"},"researchTitle":{"en":"Establishment of human trophoblast stem-like cells derived from human induced pluripotent stem cells for studying human placental development"},"periodStart":"2021-05-31","periodEnd":"2023-07-05","datasets":["JGAD000121"]},{"principalInvestigator":{"en":"Danny Leung"},"affiliation":{"en":"Division of Life Science, The Hong Kong University of Science and Technology"},"country":{"ja":"香港","en":"Hong Kong"},"researchTitle":{"en":"Defining the transcriptional regulatory roles of retrotransposons in human placenta cells."},"periodStart":"2021-08-11","periodEnd":"2027-05-28","datasets":["JGAD000121"]},{"principalInvestigator":{"en":"Gianluca Ursini"},"affiliation":{"en":"Lieber Institute for Brain Development, Johns Hopkins University School of Medicine"},"country":{"ja":"アメリカ合衆国 (メリーランド州)","en":"Maryland, United States"},"researchTitle":{"en":"Analysis of the transcriptional profile of Human Trophoblast Stem Cells"},"periodStart":"2022-04-26","periodEnd":"2026-12-31","datasets":["JGAD000121"]},{"principalInvestigator":{"en":"Ming-an Sun"},"affiliation":{"en":"College of Veterinary Medicine, Yangzhou University"},"country":{"ja":"中国","en":"China"},"researchTitle":{"en":"The activation and function of endogenous retroviruses in mammalian placentae"},"periodStart":"2022-06-23","periodEnd":"2025-01-10","datasets":["JGAD000121"]},{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000121"]},{"principalInvestigator":{"en":"Xinzhi Zhao"},"affiliation":{"en":"Zhao lab  International Peace Maternal and Child Health Hospital, School of Medicine, Shanghai Jiao Tong University"},"country":{"ja":"中国","en":"China"},"researchTitle":{"en":"Research on epigenetic regulations of placenta-specific enhancers associated with preeclampsia."},"periodStart":"2022-12-19","periodEnd":"2023-10-20","datasets":["JGAD000121"]},{"principalInvestigator":{"en":"Paul Robson"},"affiliation":{"en":"Genomic Medicine, The Jackson Laboratory"},"country":{"ja":"アメリカ合衆国 (コネチカット州)","en":"Connecticut, United States"},"researchTitle":{"en":"Modeling extra-embryonic mesoderm lineage development with human induced pluripotent stem cells "},"periodStart":"2024-02-08","periodEnd":"2026-12-26","datasets":["JGAD000121"]},{"principalInvestigator":{"ja":"依馬 正次","en":"Masatsugu Ema"},"affiliation":{"ja":"幹細胞・ヒト疾患モデル研究分野, 滋賀医科大学　動物生命科学研究センター","en":"Department of Stem Cells and Human Disease Models, Shiga University of Medical Science, Research Center for Animal Life Science"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"ヒト胎盤からの栄養膜幹細胞の単離と性状解析","en":"Isolation and characterization of trophoblast stem cells from human placenta"},"periodStart":"2024-08-19","periodEnd":"2026-08-31","datasets":["JGAD000121"]}]}