{"id":"hum0106","version":1,"url":"https://humandbs.dbcls.jp/research/hum0106/v1","datePublished":"2022-09-05","versions":[{"version":1,"datePublished":"2022-09-05"}],"title":{"ja":"ヒト大腸がん培養組織を用いたがん予防・治療法の開発","en":"Development of the prevention and therapy of CRC using patient derived culture tissues."},"summary":{"aims":{"ja":"ヒト大腸がん組織の細胞多様性・階層性解析","en":"analysis of cellular diversity and hierarchy of human colorectal cancer (CRC)"},"methods":{"ja":"RNA-seqによる一細胞解析","en":"single cell RNA-seq"},"targets":{"ja":"大腸がん3症例由来の3次元培養細胞各1細胞から抽出したRNA","en":"culture tissues derived from CRC patients"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"大腸癌：3症例\n（患者由来3次元培養細胞）\n（日本人）","en":"3 patients with colorectal cancer\n(patient derived culture tissues)\n(Japanese)"},"typeOfData":{"ja":"NGS\n（scRNA-seq）","en":"NGS\n(scRNA-seq)"}},"releaseNote":{"ja":"大腸がん3症例由来の3次元培養細胞各1細胞から抽出したRNAを用いたsingle-cell RNA-Seq解析データをfastqファイルにて提供する。Chromium Single Cell 3' v1 Reagent kitによるRNAライブラリ作製後、Illumina HiSeq 2500にて50 bpを読んだ（Single-end）。","en":"RNAs were extracted from single cells of culture tissues derived from 3 CRC patients. Libraries for single-cell RNA-seq were prepared with Chromium Single Cell 3' v1 Reagent kit and scRNA-seq was performed with Illumina HiSeq 2500 (50 bp, Single-end). scRNA-seq data are provided as fastq files."},"dataProviders":[{"name":{"ja":"八尾 良司","en":"Ryoji Yao"},"organization":{"name":{"ja":"がん研究会・がん研究所・細胞生物部","en":"The Cancer Institute of JFCR"}}}],"researchProjects":[{"name":{"ja":"オルガノイドプロジェクト","en":"project orgnaoid"},"url":{"ja":[{"url":"https://www.jfcr.or.jp/laboratory/department/cell_biology/www/index.html","text":"https://www.jfcr.or.jp/laboratory/department/cell_biology/www/index.html"}],"en":[{"url":"https://www.jfcr.or.jp/laboratory/department/cell_biology/www/index.html","text":"https://www.jfcr.or.jp/laboratory/department/cell_biology/www/index.html"}]}}],"grants":[{"title":{"ja":"大腸がん幹細胞の同定と細胞階層性・可塑性の解明","en":"Analysis of cellular heterogeneity of colorectal cancer"},"agency":{"ja":"科学研究費助成事業 挑戦的萌芽研究","en":"KAKENHI Grant-in-Aid for Challenging Exploratory Research"},"grantIds":["16K14620"]},{"title":{"ja":"先進ゲノム解析研究推進プラットフォーム","en":"Platform for advanced genome science"},"agency":{"ja":"科学研究費助成事業 新学術領域研究","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas"},"grantIds":["16H06279"]}],"relatedPublications":[{"title":"Comparative Analysis of Patient-Matched PDOs Revealed a Reduction in OLFM4-Associated Clusters in Metastatic Lesions in Colorectal Cancer","doi":"https://doi.org/10.1016/j.stemcr.2021.02.012","datasets":["JGAD000208"]}],"datasets":["JGAD000208"],"controlledAccessUsers":[]}