{"id":"hum0103","version":1,"url":"https://humandbs.dbcls.jp/research/hum0103/v1","datePublished":"2018-02-27","versions":[{"version":1,"datePublished":"2018-02-27"},{"version":2,"datePublished":"2019-06-21"},{"version":3,"datePublished":"2021-07-13"},{"version":4,"datePublished":"2022-01-18"}],"title":{"ja":"シークエンス解析によるがんゲノム研究：胆道がん","en":"Genome sequencing analysis for biliary tract cancer"},"summary":{"aims":{"ja":"日本人の胆道がんにおけるゲノム変異を探索する","en":"To investigate genomic alterations of Japanese biliary tract cancers"},"methods":{"ja":"胆道がん切除切片における非腫瘍組織（正常組織）および腫瘍組織より抽出したDNA/RNAを使用したNGSライブラリーを作成後、Illumina HiSeq 2000/2500、NovaSeq 6000にて塩基配列を決定する。","en":"DNAs/RNA were extracted from biliary tract cancer and paired non-cancer (normal) tissues. NGS libaraies were prepared by using TruSeq DNA Sample Prep kit (Illumina) for whole genome sequencing (WGS), Nextera Rapid Capture kit (Illumina) / Agilent SureSelect Exome V5 kit for whole exome sequencing (WES) and KAPA RNA HyperPrep Kit / TruSeq Stranded mRNA for RNA sequencing. Sequencing was perfomred by Illumina HiSeq 2000/2500 or NovaSeq 6000."},"targets":{"ja":"胆道がん症例の腫瘍組織および非腫瘍組織のDNA/RNA、ならびに正常胆嚢の非腫瘍組織のRNA","en":"Japanese biliary tract cancer patients, normal gallbladder tissue"},"url":{"ja":null,"en":null}},"listingSummary":{},"releaseNote":{"ja":"胆道がん切除切片の非腫瘍組織（正常組織）および腫瘍組織から抽出したDNAを使用したWGS解析、および、Exome解析の結果（HiSeq 2000/2500）をFastqファイルで提供する（Paired-end）。","en":"DNAs extracted from cancer tissues and paired non-cancer (normal) tissues obtained from biliary tract cancer patients were used for WGS and Exome (Paired-end). Fastq files are provided."},"dataProviders":[{"name":{"ja":"中川 英刀","en":"Hidewaki Nakagawa"},"organization":{"name":{"ja":"理化学研究所 生命医科学研究センター","en":"RIKEN Center for Integrative Medical Sciences"}}}],"researchProjects":[],"grants":[{"title":{"ja":"肝胆膵がんの多元的オミックス解析による分子機構の解明","en":"Elucidation of molecular mechanisms of hepatobiliary and pancreatic cancer by multiomics analysis"},"agency":{"ja":"科学研究費助成事業 基盤研究（A）","en":"KAKENHI Grant-in-Aid for Scientific Research (A)"},"grantIds":["18H04049"]},{"title":{"ja":"がんゲノム解析による新規免疫療法および複合免疫療法開発のためのシーズ探索","en":"Search for seeds for the development of novel immunotherapies and combined immunotherapies by cancer genome analysis"},"agency":{"ja":"日本医療研究開発機構（AMED） 次世代がん医療創生研究事業（P-CREATE）","en":"Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP20cm0106552"]}],"relatedPublications":[{"title":"Genomic characterization of biliary tract cancers identifies driver genes and predisposing mutations","doi":"https://doi.org/10.1016/j.jhep.2018.01.009","datasets":["JGAD000117","JGAD000118"]},{"title":"Molecular Classification and Tumor Microenvironment Characterization of Gallbladder Cancer by Comprehensive Genomic and Transcriptomic Analysis","doi":"https://doi.org/10.3390/cancers13040733","datasets":["JGAD000504","JGAD000505","JGAD000506"]},{"title":"Actionability evaluation of biliary tract cancer by genome transcriptome analysis and Asian cancer knowledgebase","doi":"https://doi.org/10.18632/oncotarget.28021","datasets":["JGAD000504","JGAD000505","JGAD000506"]}],"datasets":["JGAD000117","JGAD000118"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"柴田 龍弘","en":"Tatsuhiro Shibata"},"affiliation":{"ja":"がんゲノミクス研究分野, 国立がん研究センター研究所","en":"Division of Cancer Genomics, National Cancer Center Research Institute"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"ゲノム異常解析に基づく､肝がん､膵がん､肺がん､胃がん､大腸がん､胆道がん､乳がん､食道がん､卵巣が\nん､子宮がん､膀胱がん､頭頸部がん､骨軟部肉腫の発生･進展の分子機構の解明","en":"Study the molecular mechanics of tumor development and progression with analysing genomic mutations of liver, pancreatic, lung, colorectal, biliary tract, breast,\nesophageal, ovarian, cervical, bladder, renal, head-and-neck and bone cancers."},"periodStart":"2018-09-11","periodEnd":"2018-11-28","datasets":["JGAD000117","JGAD000118"]},{"principalInvestigator":{"ja":"山本 雅之","en":"Masayuki Yamamoto"},"affiliation":{"ja":"東北メディカル・メガバンク機構, 東北大学","en":"Tohoku Medical Megabank Organization"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"日本人全ゲノムデータベースの構築","en":"Construction of Japanese whole genome database"},"periodStart":"2019-06-24","periodEnd":"2022-04-15","datasets":["JGAD000117"]},{"principalInvestigator":{"en":"Justo Lorenzo Bermejo"},"affiliation":{"en":"Statistical Genetics Research Group. Institute of Medical Biometry, Heidelberg University Hospital"},"country":{"ja":"ドイツ","en":"Germany"},"researchTitle":{"en":"Genomic characterization of gallbladder tumors from patients worldwide"},"periodStart":"2022-10-13","periodEnd":"2028-12-31","datasets":["JGAD000118","JGAD000505"]},{"principalInvestigator":{"ja":"山本 拓也","en":"Takuya Yamamoto"},"affiliation":{"ja":"プレシジョン免疫プロジェクト, 国立研究開発法人 医薬基盤・健康・栄養研究所","en":"Center for Intractable Diseases and ImmunoGenomics, National Institutes of Biomedical Innovation, Health and Nutrition"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"新規がん抗原の同定に基づく消化器がんおよび悪性黒色腫（メラノーマ）に対するワクチン開発基盤研究\r\n","en":"Basic research for vaccine development against gastrointestinal cancer and malignant melanoma based on the identification of novel cancer antigens\r\n"},"periodStart":"2024-11-12","periodEnd":"2026-03-31","datasets":["JGAD000117","JGAD000118","JGAD000504","JGAD000505","JGAD000506"]}]}