{"id":"hum0096","version":1,"url":"https://humandbs.dbcls.jp/research/hum0096/v1","datePublished":"2017-05-25","versions":[{"version":1,"datePublished":"2017-05-25"},{"version":2,"datePublished":"2019-11-18"}],"title":{"ja":"造血器腫瘍における遺伝子異常の網羅的解析（小児T細胞性急性リンパ性白血病）","en":"Comprehensive analysis of genetic alterations in hematological malignancies"},"summary":{"aims":{"ja":"多数の小児T細胞性急性リンパ芽球性白血病（小児T-ALL）検体について、次世代シーケンサーを用いた種々の遺伝子解析技術により、疾患発症の原因となる遺伝子異常をゲノムワイドに同定し、解析する。","en":"To explore genetic alteratoins in pediatric T-cell acute lymphoblastic leukemia (T-ALL), we performed genome wide analysis using next generation sequencing methods."},"methods":{"ja":"小児T-ALL123例の検体のRNA-seq解析を行い、融合遺伝子を同定し、発現データの解析をする。うち、79例に対してDNAメチル化アレイ解析を行い、発現データと統合的に解析をする。","en":"We performed whole transcriptome sequencing in 123 pediatric T-ALL for detecting gene fusion and analysing expression data. Among them, 79 cases were further analyzed in DNA metylation array."},"targets":{"ja":"小児T-ALL 123症例","en":"123 pediatric T-ALL patients"},"url":{"ja":null,"en":null}},"listingSummary":{},"releaseNote":{"ja":"小児T細胞性急性リンパ芽球性白血病（小児T-ALL）患者の骨髄液や末梢血、リンパ節から抽出したtotal RNAを使用し、RNA-seq解析した結果をbamファイルにて提供する。illumina TruSeq RNA Library Prep KitまたはNEB NEBNext Ultra RNA Library Prep Kit for IlluminaによるRNAライブラリ作製後、HiSeq 2500（Illumina）にて平均長108 bpを読んだ（Paired-end）。","en":"Total RNAs were extracted from bone marrow aspirates, peripheral blood cells, or lymph nodes of pediatric T-ALL patients. Libraries for RNA-seq were prepared using Illumina TruSeq RNA Library Prep Kit or NEB NEBNext Ultra RNA Library Prep Kit for Illumina and RNA-seq was performed with the Illumina HiSeq 2500 sequencer (108 bp, Paired-end). The sequences are provided as bam files."},"dataProviders":[{"name":{"ja":"滝田 順子","en":"Junko Takita"},"organization":{"name":{"ja":"東京大学大学院 医学系研究科 生殖・発達・加齢医学専攻 小児医学講座","en":"Department of Pediatrics, Graduate School of Medicine, The University of Tokyo"}}}],"researchProjects":[],"grants":[{"title":{"ja":"分子プロファイリングを基盤とした小児期からAYA世代に発症する難治がんの新規治療法 の開発","en":"Development for novel therapeutic stratagies of intractable cancers in children and adolescent and young adult using molecular profilings"},"agency":{"ja":"日本医療研究開発機構（AMED） 次世代がん医療創生研究事業（P-CREATE）","en":"Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP16cm0106509"]},{"title":{"ja":"マルチオミックス情報を基盤とした難治性小児がんに対する新規克服法の開発","en":"Development for novel therapeutic stratagies of intractable pediatric cancers based on the multi-omics analysis"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["17H04224"]}],"relatedPublications":[{"title":"Recurrent PU.1 (SPI1) fusions in high-risk pediatric T cell acute lymphoblastic leukemia","doi":"https://doi.org/10.1038/ng.3900","datasets":["JGAD000090"]},{"title":"DNA methylation-based classification reveals difference between pediatric T-cell acute lymphoblastic leukemia and normal thymocytes","doi":"https://doi.org/10.1038/s41375-019-0626-2","datasets":["JGAD000207"]}],"datasets":["JGAD000090"],"controlledAccessUsers":[{"principalInvestigator":{"en":"Jinyan Huang"},"affiliation":{"en":"State Key Laboratory Of Medical Genomics, Rui-jin Hospital"},"country":{"ja":"中国","en":"China"},"researchTitle":{"ja":"Comparing analysis adult and Pediatric B-cell Acute Lymphoblastic Leukemia","en":"Comparing analysis adult and Pediatric B-cell Acute Lymphoblastic Leukemia"},"periodStart":"2017-07-19","periodEnd":"2023-07-13","datasets":["JGAD000090"]},{"principalInvestigator":{"en":"Olivier BERNARD"},"affiliation":{"en":"Institut Gustave Roussy"},"country":{"ja":"フランス","en":"France"},"researchTitle":{"ja":"Normal and malignant hematopoiesis (Hématopoiese normale et pathologique)","en":"Normal and malignant hematopoiesis (Hématopoiese normale et pathologique)"},"periodStart":"2017-09-14","periodEnd":"2021-03-19","datasets":["JGAD000090"]},{"principalInvestigator":{"en":"Jun Yang"},"affiliation":{"en":"Department of Pharmaceutical Science, St. Jude Children's Research Hospital"},"country":{"ja":"アメリカ合衆国 (テネシー州)","en":"Tennessee, United States"},"researchTitle":{"ja":"GENOMICS OF ANTILEUKEMIC DRUG SENSITIVITY IN ALL","en":"GENOMICS OF ANTILEUKEMIC DRUG SENSITIVITY IN ALL"},"periodStart":"2019-04-11","periodEnd":"2022-01-26","datasets":["JGAD000090"]},{"principalInvestigator":{"en":"Charles Mullighan"},"affiliation":{"en":"Pathology Department, St. Jude Children's Research Hospital"},"country":{"ja":"アメリカ合衆国 (テネシー州)","en":"Tennessee, United States"},"researchTitle":{"ja":"Genomic analysis of acute lymphoblastic leukemia","en":"Genomic analysis of acute lymphoblastic leukemia"},"periodStart":"2019-10-11","periodEnd":"2024-12-05","datasets":["JGAD000090","JGAD000207"]},{"principalInvestigator":{"en":"Takaomi Sanda"},"affiliation":{"en":"Cancer Science Institute of Singapore, National University of Singa"},"country":{"ja":"シンガポール","en":"Singapore"},"researchTitle":{"ja":"Sequencing and Expression Analyses in T-Cell Leukemia Using Publicly-Available Datasets","en":"Sequencing and Expression Analyses in T-Cell Leukemia Using Publicly-Available Datasets"},"periodStart":"2020-07-14","periodEnd":"2024-09-30","datasets":["JGAD000090","JGAD000207"]},{"principalInvestigator":{"en":"Charles de Bock"},"affiliation":{"en":"Children's Cancer Institute, University of New South Wales"},"country":{"ja":"オーストラリア (ニューサウスウェールズ州)","en":"New South Wales, Australia"},"researchTitle":{"ja":"Elucidating the role of the TCF7-SPI1 fusion in T cell acute lymphoblastic leukemia","en":"Elucidating the role of the TCF7-SPI1 fusion in T cell acute lymphoblastic leukemia"},"periodStart":"2020-08-17","periodEnd":"2021-08-18","datasets":["JGAD000090"]},{"principalInvestigator":{"en":"Jinyan Huang"},"affiliation":{"en":"Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. Biomedical big data center, the First Affiliated Hospital, Zhejiang University"},"country":{"ja":"中国","en":"China"},"researchTitle":{"en":"Comprehensive analysis of alternative splicing in malignant tumors"},"periodStart":"2022-03-15","periodEnd":"2024-01-01","datasets":["JGAD000090","JGAD000207"]},{"principalInvestigator":{"en":"Chengyu Liang"},"affiliation":{"en":"The Wistar institute"},"country":{"ja":"アメリカ合衆国 (ペンシルベニア州)","en":"Pennsylvania, United States"},"researchTitle":{"en":"Oncogenic mechanism of T-cell lymphoblastic leukemia"},"periodStart":"2022-01-14","periodEnd":"2027-11-22","datasets":["JGAD000090","JGAD000207"]},{"principalInvestigator":{"ja":"安田 貴彦","en":"Takahiko Yasuda"},"affiliation":{"ja":"臨床研究センター, 名古屋医療センター","en":"Clinical Researc Center, Nagoya Medical Center"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"造血器疾患の分子病態の解明ならびに有効かつ安全性の高い治療法開発を目指した基盤研究","en":"Basic research aimed at elucidating the molecular pathology and developing effective and safe treatment methods for hematological diseases"},"periodStart":"2022-01-13","periodEnd":"2030-03-31","datasets":["JGAD000090"]},{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000090","JGAD000207"]}]}