{"id":"hum0095","version":1,"url":"https://humandbs.dbcls.jp/research/hum0095/v1","datePublished":"2018-07-30","versions":[{"version":1,"datePublished":"2018-07-30"}],"title":{"ja":"大腸前がん病変および早期大腸がんにおける遺伝子解析および発がん機序の解明","en":"Genetic analysis of colorectal precancerous lesion and early colorectal cancer to investigate mechanism of carcinogenesis of colorectal cancer"},"summary":{"aims":{"ja":"大腸における発がん早期の遺伝子変異の特徴を明らかにし、発がん機序を解明する","en":"to investigate the genomic signature and the mechanism of early stage of colorectal carcinogenesis"},"methods":{"ja":"診断に影響のない範囲で切除標本の腫瘍組織および非腫瘍組織（正常粘膜：症例コントロール）を採取した上でDNAを抽出し全エクソームシーケンスを実施した。","en":"DNAs were extracted from tumor tissues and matched normal samples and performed whole exome sequencing"},"targets":{"ja":"大腸腺腫もしくは大腸がん早期病変 計10症例","en":"10 cases of colorectal adenomas or precancerous lesions of colorectal cancers (PCRCs) in total"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"大腸腺腫、大腸癌早期病変：計10症例\n（日本人）","en":"10 cases of colorectal adenomas or PCRCs\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome）","en":"NGS\n(Exome)"}},"releaseNote":{"ja":"大腸腺腫もしくは大腸がん早期病変の腫瘍組織と非腫瘍組織（正常大腸粘膜組織）より抽出したDNAを使用したExome解析の結果をfastqファイルにて提供する。SureSelect Human All Exon V5によりExon領域にしぼり、HiSeq 2500（Illumina）にて平均長100塩基を解読した（Paired-end）。","en":"DNAs extracted from tumor tissues and non-tumor tissues from 10 cases of colorectal adenomas or precancerous lesions of colorectal cancers (PCRCs) were used for the whole exome sequencing (fastq files). Exons were narrowed down by using of SureSelect Human ALL Exon V5 and read by HiSeq 2500 (Illumina) (Paired-end: 100 bp)."},"dataProviders":[{"name":{"ja":"三森 功士","en":"Koshi Mimori"},"organization":{"name":{"ja":"九州大学病院 別府病院 外科","en":"Department of Surgery, Kyushu University Beppu Hospital"}}}],"researchProjects":[],"grants":[{"title":{"ja":"次世代シーケンサーによる大腸前がん病変からがんへの進化に関わる遺伝子の解明","en":"Investigation into the genes associated with the colorectal cancer evolution in the early stage of carcinogenesis"},"agency":{"ja":"科学研究費助成事業 若手研究（B）","en":"KAKENHI Grant-in-Aid for Young Scientists (B)"},"grantIds":["16K19107"]}],"relatedPublications":[{"title":"A temporal shift of the evolutionary principle shaping intratumor heterogeneity in colorectal cancer","doi":"https://doi.org/10.1038/s41467-018-05226-0","datasets":["JGAD000092"]}],"datasets":["JGAD000092"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"白石 航也","en":"Kouya Shiraishi"},"affiliation":{"ja":"ゲノム生物学研究分野, 国立研究開発法人国立がん研究センター研究所","en":"Division of Genome Biology, National Cancer Research Institute"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"ゲノム解析に基づく宿主並びに腫瘍における免疫応答ネットワーク機構の解明","en":"Elucidation of immune-system networks between host and tumor based on genomic analysis"},"periodStart":"2019-08-05","periodEnd":"2029-03-31","datasets":["JGAD000092"]}]}