{"id":"hum0088","version":2,"url":"https://humandbs.dbcls.jp/research/hum0088/v2","datePublished":"2019-09-10","versions":[{"version":1,"datePublished":"2019-02-08"},{"version":2,"datePublished":"2019-09-10"}],"title":{"ja":"腫瘍細胞におけるTGF-βファミリーシグナルの網羅的解析","en":"Global analysis of TGF-beta family signaling in cancer cells"},"summary":{"aims":{"ja":"transforming growth factor (TGF)-βスーパーファミリーのサブファミリーの1つであるbone morphogenetic protein（BMP）がグリオーマ幹細胞に与える影響と、BMPによるグリオーマ幹細胞の制御メカニズムを明らかにする。","en":"To elucidate the effect and its mechanism of BMP signaling on glioma initiating cells"},"methods":{"ja":"BMPの標的遺伝子を同定するため、神経膠芽腫（glioblastoma）由来細胞株より抽出したDNAを用いたwhole genome sequencing（WGS）およびRNAを用いたRNA-seq解析を実施。","en":"Target genes were identified by RNA-seq to elucidate the mechanism of regulation of glioma initiating cells by BMP. Genomic DNA was extracted from glioma initiating cells and whole genome sequencing was performed."},"targets":{"ja":"神経膠芽腫由来細胞株 2株","en":"Patient-derived glioblastoma cell lines (tumor initiating cells), 2 lines"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"発現\n配列決定","en":"Expression profiling, Sequencing"},"targets":{"ja":"脳腫瘍：2症例\n（日本人）","en":"2 glioblastomas\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq、WGS）","en":"NGS\n(RNA-seq, WGS)"}},"releaseNote":{"ja":"1症例の神経膠芽腫由来細胞（TGS-01）から抽出したmRNAを使用したRNA-seqデータ（fastqファイル）を追加した。","en":"RNA-seq data from 1 patient-derived glioblastoma cell line (tumor initiating cells) (TGS-01) were added (fastq format)."},"dataProviders":[{"name":{"ja":"宮園 浩平","en":"Kohei Miyazono"},"organization":{"name":{"ja":"東京大学大学院 医学系研究科 分子病理学分野","en":"Department of Molecular Pathology, Graduate School of Medicine, The University of Tokyo"}}}],"researchProjects":[],"grants":[{"title":{"ja":"TGF-βファミリーのがん微小環境に及ぼす作用とがん治療戦略","en":"Effects of TGF-β family on cancer microenvironment and strategies for cancer therapy"},"agency":{"ja":"科学研究費助成事業 新学術領域研究","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas"},"grantIds":["22112002"]},{"title":{"ja":"未分化性維持におけるBMPシグナルの役割","en":"Roles of BMP signaling in maintenance of cell stemness"},"agency":{"ja":"科学研究費助成事業 基盤研究（B）","en":"KAKENHI Grant-in-Aid for Scientific Research (B)"},"grantIds":["24390070"]},{"title":{"ja":"TGF-βシグナルによる転写調節とがん悪性化機構","en":"Transcriptional regulation by TGF-β signaling and its relation to progression of cancer"},"agency":{"ja":"科学研究費助成事業 基盤研究（S）","en":"KAKENHI Grant-in-Aid for Scientific Research (S)"},"grantIds":["15H05774"]},{"title":{"ja":"EphA6 tyrosine kinase as a potential biomarker for BMP-based therapy in adult glioblastoma","en":"EphA6 tyrosine kinase as a potential biomarker for BMP-based therapy in adult glioblastoma Research Project"},"agency":{"ja":"科学研究費助成事業 若手研究","en":"KAKENHI Grant-in-Aid for Early-Career Scientists"},"grantIds":["19K16735"]},{"title":{"ja":"TGF-βファミリーによる細胞分化とダイバーシティー獲得の分子基盤","en":"Molecular mechanisms of cell differentiation and diversity by TGF-beta family members"},"agency":{"ja":"科学研究費助成事業 新学術領域研究（研究領域提案型）","en":"KAKENHI Grant-in-Aid for Scientific Research on Innovative Areas (Research in a proposed research area)"},"grantIds":["17H06326"]},{"title":{"ja":"TGF-βファミリーシグナル国際共同研究拠点","en":"Cooperative International Framework in TGF-beta Family Signaling"},"agency":{"ja":"日本学術振興会 先端研究拠点事業 国際戦略型","en":"JSPS Core-to-Core Program"},"grantIds":null}],"relatedPublications":[{"title":"Bone morphogenetic protein signaling mediated by ALK-2 and DLX2 regulates apoptosis in glioma-initiating cells.","doi":"https://doi.org/10.1038/onc.2017.112","datasets":["JGAD000077"]},{"title":"Identification of a novel fusion gene HMGA2-EGFR in glioblastoma.","doi":"https://doi.org/10.1002/ijc.31179","datasets":["JGAD000077","JGAD000096"]},{"title":"Tyrosine kinase Eph receptor A6 sensitizes glioma-initiating cells towards bone morphogenetic protein-induced apoptosis.","doi":"https://doi.org/10.1111/cas.14187","datasets":["JGAD000263"]}],"datasets":["JGAD000077","JGAD000263","JGAD000096"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000077","JGAD000263"]}]}