{"id":"hum0065","version":1,"url":"https://humandbs.dbcls.jp/research/hum0065/v1","datePublished":"2018-04-06","versions":[{"version":1,"datePublished":"2018-04-06"}],"title":{"ja":"患者腫瘍移植モデルとex vivo培養間のシャトルシステムによる革新的な臨床効果評価技術の開発","en":"Shuttle system between petient-derived xenograft and ex vivo culture for innovative platform of evaluating drug sensitivity"},"summary":{"aims":{"ja":"大腸がん、肺がん、子宮頸部小細胞がんについて、患者間の多様性をカバーするCTOS（cancer tissue-originated spheroid）パネルを構築し、CTOSの調製、培養、細胞株化、移植、継代における質的変化を病理学的、生化学的、分子生物学的に検証する。","en":"This study aimed at establishment of CTOS (cancer tissue-originated spheroid) panel covering intra-patient heterogeneity for colorectal cancer, lung cancer and small cell carcinoma of the cervix. With the panel, changes in preparation, culture, and xenograft were pathologically, biochemically, and molecular biologically monitored."},"methods":{"ja":"Illumina HiSeq 2000を使用したExome解析","en":"Exome analysis by using Illumina HiSeq 2000"},"targets":{"ja":"大腸がん、肺がん、子宮頸部小細胞がん","en":"Colorectal cancer, Lung cancer, Small cell carcinoma of uterine cervix"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"大腸癌：8症例\n肺癌：2症例\n子宮頸部小細胞癌：2症例\n（日本人）","en":"8 colorectal cancer\n2 lung cancer\n2 small cell carcinoma of uterine cervix\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome）","en":"NGS\n(Exome)"}},"releaseNote":{"ja":"大腸がん、肺がん、子宮頸部小細胞がん症例由来CTOSパネルを使用したExome解析の結果をFastqファイルにて提供する。SureSelect Human All Exon V5 + lincRNAを用いてExon領域にしぼりIllumina HiSeq 2000にて平均長100塩基のDNA断片を解読した（Paired-end）。","en":"CTOS panel derived from colorectal cancer, lung cancer and small cell carcinoma of uterine cervix patients was used for the whole exome sequencing (fastq files). Exons were narrowed down by using of SureSelect Human All Exon V5 + lincRNA and read by Illumina HiSeq 2000 (paired-end: 100 bp)."},"dataProviders":[{"name":{"ja":"井上 正宏","en":"Masahiro Inoue"},"organization":{"name":{"ja":"大阪国際がんセンター 生化学部","en":"Department of Biochemistry, Osaka International Cancer Institute"}}}],"researchProjects":[],"grants":[{"title":{"ja":"患者腫瘍移植モデルとex vivo培養間のシャトルシステムによる革新的な臨床効果評価技術の開発","en":"Development of an innovative drug evaluation system using a shuttle system between patient-derived tumors and an ex vivo assay"},"agency":{"ja":"日本医療研究開発機構（AMED） 次世代がん研究シーズ戦略的育成プログラム（P-DIRECT）","en":"Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP15cm0106155"]}],"relatedPublications":[],"datasets":["JGAD000089"],"controlledAccessUsers":[]}