{"id":"hum0053","version":2,"url":"https://humandbs.dbcls.jp/research/hum0053/v2","datePublished":"2020-04-14","versions":[{"version":1,"datePublished":"2016-07-20"},{"version":2,"datePublished":"2020-04-14"}],"title":{"ja":"悪性中皮腫のプロファイリングによる新規分子標的の同定","en":"Identification of new molecular targets with profiling of malignant mesothelioma"},"summary":{"aims":{"ja":"アスベスト曝露によって発症する悪性胸膜中皮腫の網羅的ゲノム解析により新規分子標的治療法候補分子の同定を行うとともに、新たな分子診断・治療法の開発を目指す","en":"Malignant pleural mesothelioma which is associated with asbestos exposure is highly aggressive and refractory to conventional therapeutic modalities. The aim of this study is identification of new targets for molecular target therapy by next generation sequencing and application of the genomic data to new diagnostic and therapeutic strategies for malignant pleural mesothelioma patients."},"methods":{"ja":"Whome Exome Sequencing（Exome）：悪性胸膜中皮腫患者の外科手術検体あるいは樹立した中皮腫細胞株、および、同一症例の末梢血より抽出したゲノムDNAを用いて全エクソームシークエンス解析を行った。\nWhole Genome Sequencing（WGS）：悪性胸膜中皮腫患者の外科手術検体（腫瘍組織）より樹立した中皮腫細胞株より抽出したDNAを対象としたWGS解析を、large structral variantsを同定するために10X Chromiumを用いて行った。","en":"Exome: We extracted genomic DNAs from surgical specimens or established mesothelioma cell lines from Japanese malignant pleural mesothelioma patients. We also extracted control DNAs from peripheral blood of the same patients. We performed whole exome sequencing using these DNAs.\nWGS: We extracted genomic DNAs from established mesothelioma cell lines from Japanese malignant pleural mesothelioma patients. We sequenced those genomic DNAs using 10X Genomics sequencing technologies, and analyzed genomic structural variations."},"targets":{"ja":"悪性胸膜中皮腫38症例 （患者由来中皮腫細胞株12例を含む）","en":"38 Malignant pleural mesothelioma patients (including 12 established mesothelioma cell lines)"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"悪性胸膜中皮腫：38症例\n（日本人）","en":"38 Malignant pleural mesothelioma\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome、WGS）","en":"NGS\n(Exome, WGS)"}},"releaseNote":{"ja":"日本人の悪性胸膜中皮腫患者の外科手術検体から樹立した中皮腫細胞株より抽出したゲノムDNAを用いた10x Genomics Chromium ControllerによるLong Read Whole Genome Sequencingデータ（fastq）およびサンプルごとのStructural Variantsデータ（vcf, bed）を提供する。","en":"DNAs extracted from established mesothelioma cell lines from Japanese malignant pleural mesothelioma patients were sequenced using 10X Genomics sequencing technologies. Fastq files and vcf/bed files for structural variants are provided."},"dataProviders":[{"name":{"ja":"中野 孝司","en":"Takashi Nakano"},"organization":{"name":{"ja":"兵庫医科大学 呼吸器内科","en":"Hyogo College of Medicine, Division of Respiratory Medicine"}}}],"researchProjects":[{"name":{"ja":"次世代がん研究シーズ戦略的育成プログラム","en":"Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT)"},"url":{"ja":[{"url":"https://hdc-tsrg.com/","text":"https://hdc-tsrg.com/"}],"en":[{"url":"https://www.pref.aichi.jp/cancer-center/english/ri/01division/03cancer_biology/index.html","text":"https://www.pref.aichi.jp/cancer-center/english/ri/01division/03cancer_biology/index.html"}]}}],"grants":[{"title":{"ja":"分子プロファイリングによる新規標的同定を通じた難治がん治療法開発（悪性中皮腫のプロファイリングによる新規分子標的の同定）","en":"Development of therapeutic modalities against intractable cancers via identification of new therapeutic targets with molecular profiling (Identification of new molecular targets with profiling of malignant mesothelioma)"},"agency":{"ja":"日本医療研究開発機構（AMED） 次世代がん研究シーズ戦略的育成プログラム（P-DIRECT）","en":"Project for Development of Innovative Research on Cancer Therapeutics (P-DIRECT), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP15cm0106068"]},{"title":{"ja":"悪性中皮腫のゲノム異常と代謝・細胞特性の包括的理解による新規分子標的の同定","en":"Identification of novel molecular targets by comprehensive understanding of genomic abnormalities and metabolic and cellular characteristics of malignant mesothelioma"},"agency":{"ja":"日本医療研究開発機構（AMED） 次世代がん医療創生研究事業（P-CREATE）","en":"Project for Cancer Research and Therapeutic Evolution (P-CREATE), Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP19cm0106606"]}],"relatedPublications":[{"title":"Integrative molecular characterization of malignant pleural mesothelioma.","doi":"https://doi.org/10.1158/2159-8290.CD-18-0804","datasets":["JGAD000062"]}],"datasets":["JGAD000062","JGAD000256"],"controlledAccessUsers":[{"principalInvestigator":{"ja":"白石 航也","en":"Kouya Shiraishi"},"affiliation":{"ja":"ゲノム生物学研究分野, 国立研究開発法人国立がん研究センター研究所","en":"Division of Genome Biology, National Cancer Research Institute"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"ゲノム解析に基づく宿主並びに腫瘍における免疫応答ネットワーク機構の解明","en":"Elucidation of immune-system networks between host and tumor based on genomic analysis"},"periodStart":"2019-08-05","periodEnd":"2029-03-31","datasets":["JGAD000062"]},{"principalInvestigator":{"ja":"有田 正規","en":"Masanori Arita"},"affiliation":{"ja":"生命情報・DDBJ センター, 国立遺伝学研究所","en":"Bioinformation and DDBJ Center, National Institute of Genetics"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"DDBJ センターと NBDC による JGA 解析データの提供","en":"Provision of processed JGA data analyzed by DDBJ Center and NBDC"},"periodStart":"2021-10-07","periodEnd":"2030-03-31","datasets":["JGAD000062"]}]}