{"id":"hum0050","version":1,"url":"https://humandbs.dbcls.jp/research/hum0050/v1","datePublished":"2016-09-30","versions":[{"version":1,"datePublished":"2016-09-30"}],"title":{"ja":"B型肝炎創薬へ向けてのトランスクリプトーム解析による病態解明と標的同定","en":"Transcriptome analysis of Hepatitis B for drug discovery and clinical applications"},"summary":{"aims":{"ja":"HBV感染者の血液のトランスクリプトームを理解する。","en":"Understand blood transcriptome of HBV positive blood."},"methods":{"ja":"血液からRNAを抽出し、Cap Analysis of Gene Expression（CAGE）ライブラリを作成後、シークエンシングを実施","en":"Extract RNA from whole blood and prepare CAGE libraries for deep sequencing"},"targets":{"ja":"抗ウイルス療法未施行のGenotype Cの男性HBVキャリア","en":"HBV (Genotype C) positive blood samples derived from untreated patients (men)"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"転写開始点同定","en":"Transcription start site identification"},"targets":{"ja":"HBVキャリア：8症例\n（日本人）","en":"8 HBV (Genotype C) positive patients\n(Japanese)"},"typeOfData":{"ja":"NGS\n（CAGE-seq）","en":"NGS\n(CAGE-seq)"}},"releaseNote":{"ja":"抗ウイルス療法未施行のGenotype Cの男性HBVキャリアの血液より抽出したRNAを使用してCAGEライブラリを作成し、Illumina HiSeq 2000によるシークエンシングを行った結果をfastqおよびbamファイルにて提供する。","en":"RNA extracted from HBV (Genotype C) positive whole blood samples derived from untreated patients (men) were used for preparation of CAGE libraries. Deep sequencing analysis on the libraries was performed with the Illumina HiSeq 2000 sequencer (47 bp, single-end) and provided in fastq and bam format files (Reference: hg19)."},"dataProviders":[{"name":{"ja":"松浦 知和","en":"Tomokazu Matsuura"},"organization":{"name":{"ja":"東京慈恵会医科大学 臨床検査医学講座","en":"The Jikei University School of Medicine, Rinsho Kensa Igaku Koza"}}}],"researchProjects":[],"grants":[{"title":{"ja":"次世代生命基盤技術を用いたB型肝炎制圧のための創薬研究","en":"Drug Discovery Research for Complete Cure of Chronic Hepatitis Type B Utilizing The Next Generation Life Science Technologies"},"agency":{"ja":"日本医療研究開発機構（AMED） 肝炎等克服実用化研究事業 B型肝炎創薬実用化等研究事業","en":"Division of Infectious Disease Research, Research Program on Hepatitis, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["JP15fk0310004"]}],"relatedPublications":[{"title":"Single-nucleotide-resolution mapping of HBV promoters in infected human livers and hepatocellular carcinoma","doi":"https://doi.org/10.1128/JVI.01625-16","datasets":["JGAD000053"]}],"datasets":["JGAD000053"],"controlledAccessUsers":[]}