{"id":"hum0045","version":1,"url":"https://humandbs.dbcls.jp/research/hum0045/v1","datePublished":"2016-03-29","versions":[{"version":1,"datePublished":"2016-03-29"}],"title":{"ja":"思春期・若年成人急性リンパ芽球性白血病におけるトランスクリプトーム解析","en":"Transcriptome analysis of acute lymphoblastic leukemia in adolescents and young adults"},"summary":{"aims":{"ja":"思春期・若年成人急性リンパ芽球性白血病（Acute Lymphoblastic Leukemia: ALL）の発症の原因となる遺伝子異常を同定する。","en":"Screening of oncogenic fusion genes in acute lymphoblastic leukemia (ALL) in adolescents and young adults"},"methods":{"ja":"Illumina HiSeq 2000 / 2500を使用したRNA-seq解析","en":"RNA-seq by using HiSeq 2000 / 2500"},"targets":{"ja":"フィラデルフィア染色体陰性ALL 99症例、フィラデルフィア染色体陽性ALL 3症例、セルライン 5検体、正常血球細胞 8検体","en":"99 Philadelphia chromosome-negative (Ph-) ALLs, 3 Philadelphia chromosome-positive (Ph+) ALLs, 5 cell lines, 8 normal hematopoietic cells"},"url":{"ja":[{"url":"https://www.ncc.go.jp/jp/ri/division/genetics/index.html","text":"https://www.ncc.go.jp/jp/ri/division/genetics/index.html"}],"en":[{"url":"https://www.ncc.go.jp/en/ri/division/genetics/index.html","text":"https://www.ncc.go.jp/en/ri/division/genetics/index.html"}]}},"listingSummary":{"methods":{"ja":"発現","en":"Expression profiling"},"targets":{"ja":"フィラデルフィア染色体陰性ALL：99症例\nフィラデルフィア染色体陽性ALL：3症例\nセルライン：5検体\n正常血球細胞：8検体\n（日本人）","en":"99 Ph(-) ALL\n3 Ph(+) ALL\n5 cell lines\n8 normal hematopoietic cells\n(Japanese)"},"typeOfData":{"ja":"NGS\n（RNA-seq）","en":"NGS\n(RNA-seq)"}},"releaseNote":{"ja":"フィラデルフィア染色体陰性または陽性ALL症例の末梢血、もしくは骨髄液から抽出したmRNAと、セルラインおよび正常血球細胞の培養細胞から抽出したmRNAを使用し、RNA-Seq解析した結果をFastqファイルにて提供する。NEBNext Ultra Directional RNA Library Prep Kit for Illuminaによるライブラリ作製後、HiSeq 2000もしくは2500にて平均長104塩基のDNA断片を解読した（Paired-end）。","en":"mRNAs extracted from\n(1) peripheral blood cells and bone marrow aspirates of Philadelphia chromosome-negative / -positive ALL cases\n(2) cell lines and normal hematopoietic cells\nwere used for RNA-seq (Fastq files).\nThe NEBNext Ultra Directional RNA Library Prep Kit for Illumina was used for preparing libraries and read by Illumina HiSeq 2000 / 2500 (paired-end, 104 bp)."},"dataProviders":[{"name":{"ja":"間野 博行","en":"Hiroyuki Mano"},"organization":{"name":{"ja":"国立がん研究センター研究所 細胞情報学分野","en":"Division of Cellular Signaling, National Cancer Center Research Institute"}}}],"researchProjects":[],"grants":[{"title":{"ja":"がん治療標的探索プロジェクト","en":"Projects for exploration of therapeutic targets in cancer"},"agency":{"ja":"日本医療研究開発機構（AMED） 革新的先端研究開発支援事業 インキュベートタイプ（LEAP）","en":"Leading Advanced Projects for medical innovation (LEAP), Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED)"},"grantIds":["15653406"]}],"relatedPublications":[{"title":"Recurrent DUX4 fusions in B-cell acute lymphoblastic leukemia of adolescents and young adults","doi":"https://doi.org/10.1038/ng.3535","datasets":["JGAD000047"]}],"datasets":["JGAD000047"],"controlledAccessUsers":[{"principalInvestigator":{"en":"Jinyan Huang"},"affiliation":{"en":"State Key Laboratory Of Medical Genomics, Rui-jin Hospital"},"country":{"ja":"中国","en":"China"},"researchTitle":{"ja":"Comparing analysis adult and Pediatric B-cell Acute Lymphoblastic Leukemia","en":"Comparing analysis adult and Pediatric B-cell Acute Lymphoblastic Leukemia"},"periodStart":"2016-05-23","periodEnd":"2017-03-27","datasets":["JGAD000047"]},{"principalInvestigator":{"en":"Robert Bradley"},"affiliation":{"en":"Public Health Sciences, Fred Hutchinson Cancer Research Center"},"country":{"ja":"アメリカ合衆国 (ワシントン州)","en":"Washington, United States"},"researchTitle":{"ja":"Repeat derepression and RNA-mediated toxicity in FSHD","en":"Repeat derepression and RNA-mediated toxicity in FSHD"},"periodStart":"2016-08-05","periodEnd":"2027-04-09","datasets":["JGAD000047"]},{"principalInvestigator":{"en":"Christine Harrison"},"affiliation":{"en":"Northern Institute for Cancer Research, Newcastle University"},"country":{"ja":"イギリス","en":"United Kingdom"},"researchTitle":{"ja":"Re-analysis of Publically Available RNA-Seq Data from Patients with B-cell Precursor Acute Lymphoblastic Leukaemia","en":"Re-analysis of Publically Available RNA-Seq Data from Patients with B-cell Precursor Acute Lymphoblastic Leukaemia"},"periodStart":"2019-05-07","periodEnd":"2025-05-01","datasets":["JGAD000047"]},{"principalInvestigator":{"en":"Jinyan Huang"},"affiliation":{"en":"Zhejiang University School of Medicine, 79 Qingchun Road, Hangzhou, 310003, Zhejiang, China. Biomedical big data center, the First Affiliated Hospital, Zhejiang University"},"country":{"ja":"中国","en":"China"},"researchTitle":{"en":"Comprehensive analysis of alternative splicing in malignant tumors"},"periodStart":"2022-03-15","periodEnd":"2024-01-01","datasets":["JGAD000047"]},{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000047"]}]}