{"id":"hum0044","version":1,"url":"https://humandbs.dbcls.jp/research/hum0044/v1","datePublished":"2016-10-31","versions":[{"version":1,"datePublished":"2016-10-31"}],"title":{"ja":"ヒトレトロウイルス感染症に関する研究","en":"Research about retrovirus infection"},"summary":{"aims":{"ja":"成人T細胞白血病（ATL）細胞株（ED）のエピゲノムを調べる","en":"Epigenetic analysis of adult T-cell leukemia-lymphoma (ATL) cell line"},"methods":{"ja":"クロマチン免疫沈降後シークエンス","en":"ChIP-seq"},"targets":{"ja":"ATL細胞株ED","en":"ATL cell line; ED"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"ヒストン修飾","en":"Histone modification profiling"},"targets":{"ja":"ATL細胞株：1検体\n（細胞株）","en":"1 ED (ATL cell line)\n(Cell-line)"},"typeOfData":{"ja":"NGS\n（ChIP-seq）","en":"NGS\n(ChIP-seq)"}},"releaseNote":{"ja":"成人T細胞白血病（ATL）罹患者の細胞から樹立したセルライン（ATL細胞株ED）を使用したChIP-seqデータをFastqファイルにて提供する。CTCF遺伝子に対する抗体、ヒストンに対する抗体（H3K36me3、H3K4me3、H3K9ac、H2A.Z）、IgG（コントロール）を使用したクロマチン免疫沈降後ChIP-Seqライブラリを作製し、Illumina NextSeq 500によるシーケンシングを行っている。","en":"One chromatin DNA sample extracted from an ALT cell line (ED) was used for ChIP-Seq analysis (fastq format files).\nAfter immunoprecipitation using anti-CTCF, anti-histon (H3K36me3, H3K4me3, H3K9ac, H2A.Z), and rabbit polyclonal control IgG, ChIP-Seq libraries were sequenced using Illumina NextSeq 500 platform."},"dataProviders":[{"name":{"ja":"佐藤 賢文","en":"Satou Yorifumi"},"organization":{"name":{"ja":"熊本大学 国際先端医学機構","en":"International Research Center for Medical Sciences, Kumamoto University"}}}],"researchProjects":[{"name":{"ja":"佐藤研究室","en":"Satou Laboratory"},"url":{"ja":[{"url":"https://kumamoto-u-jrchri.jp/satou/","text":"https://kumamoto-u-jrchri.jp/satou/"}],"en":null}}],"grants":[{"title":{"ja":"成人T細胞白血病の制御へ向けたHTLV-1プロウイルス解析","en":"HTLV-1 proviral analysis for the control of ATL"},"agency":{"ja":"科学研究費助成事業 基盤研究（C）","en":"KAKENHI Grant-in-Aid for Scientific Research (C)"},"grantIds":["26461428"]},{"title":{"ja":"HTLV-1 を起点とした成人 T 細胞白血病発症メカ ニズム解明と早期診断法の確立","en":"Elucidation of the mechanisms underlying HTLV-1-mediated ATL leukemogenesis"},"agency":{"ja":"公益財団法人 武田科学振興財団","en":"Takeda Science Foundation"},"grantIds":null}],"relatedPublications":[{"title":"The retrovirus HTLV-1 inserts an ectopic CTCF-binding site into the human genome","doi":"https://doi.org/10.1073/pnas.1423199113","datasets":["DRA004162"]}],"datasets":["DRA004162"],"controlledAccessUsers":[]}