{"id":"hum0040","version":1,"url":"https://humandbs.dbcls.jp/research/hum0040/v1","datePublished":"2016-09-16","versions":[{"version":1,"datePublished":"2016-09-16"}],"title":{"ja":"生殖発生に関わる細胞のエピゲノム解析基盤研究","en":"Epigenomic analysis of the human placenta"},"summary":{"aims":{"ja":"ヒト胎盤におけるインプリント制御領域およびインプリント遺伝子の同定","en":"Genome-wide identification of parent-of-origin dependent DNA methylation and gene expression in the human placenta"},"methods":{"ja":"母親由来アリルと父親由来方アリルを区別するため、Whole Genome Sequencing （WGS）もしくはWhole Exon sequencing （WES）解析を用い、ヒト胎盤および母体血より一塩基多型情報を取得した。次に、ヒト胎盤サンプルのBisulfite sequencing（BS-seq）解析およびRNA-seq解析を行い、アリル特異的DNAメチル化および遺伝子発現解析を行った。","en":"SNPs in human placentas and maternal blood cells were identified using Whole-Genome or Exome sequencing. Allele-specific DNA methylation and gene expression analyses were performed using the human placentas."},"targets":{"ja":"ヒト胎盤および母体血","en":"Human placentas and maternal blood samples"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定\n発現\nメチル化","en":"Sequencing, expression profiling, methylation profiling"},"targets":{"ja":"ヒト胎盤：28検体\n母体血：26検体\n（日本人）","en":"28 human placenta\n26 maternal blood samples\n(Japanese)"},"typeOfData":{"ja":"NGS\n（WGS、Exome、RNA-seq、WGBS）","en":"NGS\n(WGS, Exome, RNA-seq, BS-seq)"}},"releaseNote":{"ja":"以下の検体から抽出したゲノムDNAおよびRNAを使用し、WGS、Exome、WGBS、RNA-seq解析の結果をFastqファイルにて提供する。\n・WGS （母体血：2検体、胎盤：2検体）\n・Exome （母体血：24検体、胎盤：24検体）\n・WGBS （胎盤：15検体）\n・RNA-seq （胎盤：21検体）","en":"gDNAs and RNA extracted from human placentas or maternal blood cells obtained from following patients were used for the WGS, Exome, WGBS and RNA-seq (fastq files).\n1) WGS (2 human placentas and 2 maternal blood cells)\n2) Exome (24 human placentas and 24 maternal blood cells)\n3) WGBS (15 human placentas)\n4) RNA-seq (21 human placentas)"},"dataProviders":[{"name":{"ja":"有馬 隆博","en":"Takahiro Arima"},"organization":{"name":{"ja":"東北大学大学院 医学系研究科 環境遺伝医学総合研究センター 情報遺伝学分野","en":"Department of Informative Genetics, Environment and Genome Research Center, Tohoku University Graduate School of Medicine"}}}],"researchProjects":[{"name":{"ja":"生殖発生にかかわる細胞のエピゲノム解析基盤研究","en":"Epigenome Analysis of Cells in the Placenta and Endometrium Forming the Fetal-maternal Interface"},"url":{"ja":null,"en":null}}],"grants":[{"title":{"ja":"生殖発生にかかわる細胞のエピゲノム解析基盤研究","en":"Epigenome analysis of cells in the placenta and endometrium forming the fetal-maternal interface"},"agency":{"ja":"日本医療研究開発機構 革新的先端研究開発支援事業（AMED-CREST）","en":"Core Research and Evolutional Science and Technology, Advanced Research & Development Programs for Medical Innovation, Japan Agency for Medical Research and Development (AMED-CREST)"},"grantIds":["JP17gm0510011"]}],"relatedPublications":[{"title":"Allele-specific methylome and transcriptome analysis reveals widespread imprinting in the human placenta","doi":"https://doi.org/10.1016/j.ajhg.2016.08.021","datasets":["JGAD000038"]},{"title":"Derivation of Human Trophoblast Stem Cells","doi":"https://doi.org/10.1016/j.stem.2017.11.004","datasets":["JGAD000038"]}],"datasets":["JGAD000038"],"controlledAccessUsers":[{"principalInvestigator":{"en":"Martin Hirst"},"affiliation":{"en":"BC Cancer, part of the Provincial Health Services Authority"},"country":{"ja":"カナダ (ブリティッシュコロンビア州)","en":"British Columbia, Canada"},"researchTitle":{"ja":"Epigenomic analysis of human samples","en":"Epigenomic analysis of human samples"},"periodStart":"2018-08-06","periodEnd":"2022-09-05","datasets":["JGAD000038"]},{"principalInvestigator":{"en":"Zhigang Xue"},"affiliation":{"en":"School of Medicine and Life Science, Tongji University"},"country":{"ja":"中国","en":"China"},"researchTitle":{"ja":"Mechanism of cell programming and embryo-gamete original disease in human embryo development.","en":"Mechanism of cell programming and embryo-gamete original disease in human embryo development."},"periodStart":"2018-12-17","periodEnd":"2021-03-19","datasets":["JGAD000038"]},{"principalInvestigator":{"en":"Jonghwan Kim"},"affiliation":{"en":"Department of Molecular Biosciences, University of Texas at Austin"},"country":{"ja":"アメリカ合衆国 (テキサス州)","en":"Texas, United States"},"researchTitle":{"en":"Establishment of human trophoblast stem-like cells derived from human induced pluripotent stem cells for studying human placental development"},"periodStart":"2021-05-31","periodEnd":"2023-07-05","datasets":["JGAD000038"]},{"principalInvestigator":{"en":"Gianluca Ursini"},"affiliation":{"en":"Lieber Institute for Brain Development, Johns Hopkins University School of Medicine"},"country":{"ja":"アメリカ合衆国 (メリーランド州)","en":"Maryland, United States"},"researchTitle":{"en":"Analysis of the transcriptional profile of Human Trophoblast Stem Cells"},"periodStart":"2022-04-26","periodEnd":"2026-12-31","datasets":["JGAD000038"]},{"principalInvestigator":{"ja":"浜田 道昭","en":"Michiaki Hamada"},"affiliation":{"ja":"浜田研究室, 理工学術院, 早稲田大学","en":"Hamada Laboratory, Faculty of Science and Engineering, Waseda University"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"RNA標的創薬データベースの構築","en":"Construction of RNA-targeted Drug Discovery Database"},"periodStart":"2023-01-05","periodEnd":"2027-10-31","datasets":["JGAD000038"]},{"principalInvestigator":{"ja":"依馬 正次","en":"Masatsugu Ema"},"affiliation":{"ja":"幹細胞・ヒト疾患モデル研究分野, 滋賀医科大学　動物生命科学研究センター","en":"Department of Stem Cells and Human Disease Models, Shiga University of Medical Science, Research Center for Animal Life Science"},"country":{"ja":"日本","en":"Japan"},"researchTitle":{"ja":"ヒト胎盤からの栄養膜幹細胞の単離と性状解析","en":"Isolation and characterization of trophoblast stem cells from human placenta"},"periodStart":"2024-08-19","periodEnd":"2026-08-31","datasets":["JGAD000038"]}]}