{"id":"hum0039","version":1,"url":"https://humandbs.dbcls.jp/research/hum0039/v1","datePublished":"2016-12-27","versions":[{"version":1,"datePublished":"2016-12-27"}],"title":{"ja":"消化管間質腫瘍のチロシンキナーゼ阻害剤に対する獲得耐性機構の解明","en":"Analysis of TKI resistant mechanism for gastrointstinal stromal tumor (GIST)"},"summary":{"aims":{"ja":"消化管間質腫瘍のチロシンキナーゼ阻害剤に対する獲得耐性機構の解明","en":"To clarify the mechanism of TKI resistant in gastrointstinal stromal tumors"},"methods":{"ja":"腫瘍のエキソーム解析","en":"Exome DNA sequence analysis for imatinib resistant gastrointestinal stromal tumor (GIST)"},"targets":{"ja":"イマチニブに対して病理学的に耐性と診断された消化管間質腫瘍症例","en":"GIST diagnosed pathologically as a imatinib resistant lesion"},"url":{"ja":null,"en":null}},"listingSummary":{"methods":{"ja":"配列決定","en":"Sequencing"},"targets":{"ja":"イマチニブ耐性消化管間質腫瘍：4症例\n（日本人）","en":"4 Imatinib resistant GIST\n(Japanese)"},"typeOfData":{"ja":"NGS\n（Exome）","en":"NGS\n(Exome)"}},"releaseNote":{"ja":"イマチニブに対して病理学的に耐性と診断された消化管間質腫瘍4症例の腫瘍組織から抽出したDNAを用いたExome解析を行なった。\nSureSelect Human All Exon V5+UTRsを用いてExon領域にしぼり、Illumina HiSeq 1500にて平均長101bpのDNA断片を解読した（Paired-end ）。","en":"Genomic DNA was extracted from Imatinib-resistant GIST and subjected to the enrichment of exon fragments by using SureSelect Human All Exon V5+UTRs.\nIt was sequenced 101 bases from both ends of them with a HiSeq 1500 system (Illumina) with paired-end mode (fastq files)."},"dataProviders":[{"name":{"ja":"高橋 剛","en":"Tsuyoshi Takahashi"},"organization":{"name":{"ja":"大阪大学大学院 医学系研究科 外科学講座 消化器外科学","en":"Department of Surgery, Osaka university graduate school of medicine"}}}],"researchProjects":[],"grants":[],"relatedPublications":[],"datasets":["JGAD000039"],"controlledAccessUsers":[]}